Experiment: acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2760 and Shew_2761 are separated by 176 nucleotides Shew_2761 and Shew_2762 are separated by 91 nucleotides Shew_2762 and Shew_2763 overlap by 8 nucleotides
Shew_2760: Shew_2760 - isocitrate dehydrogenase (RefSeq), at 3,265,570 to 3,266,577
_2760
Shew_2761: Shew_2761 - putative RNA 2'-O-ribose methyltransferase (RefSeq), at 3,266,754 to 3,267,845
_2761
Shew_2762: Shew_2762 - hypothetical protein (RefSeq), at 3,267,937 to 3,268,326
_2762
Shew_2763: Shew_2763 - dienelactone hydrolase (RefSeq), at 3,268,319 to 3,269,041
_2763
Position (kb)
3266
3267
3268 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3266.798 kb on - strand at 3266.998 kb on + strand, within Shew_2761 at 3267.402 kb on + strand, within Shew_2761 at 3267.567 kb on - strand, within Shew_2761 at 3267.709 kb on - strand, within Shew_2761 at 3267.900 kb on - strand at 3268.332 kb on - strand at 3268.392 kb on - strand, within Shew_2763 at 3268.671 kb on + strand, within Shew_2763
Per-strain Table
Position Strand Gene LocusTag Fraction acetate (C) remove 3,266,798 - +0.5 3,266,998 + Shew_2761 0.22 +0.4 3,267,402 + Shew_2761 0.59 +0.2 3,267,567 - Shew_2761 0.74 +1.0 3,267,709 - Shew_2761 0.87 +2.1 3,267,900 - -1.1 3,268,332 - +0.1 3,268,392 - Shew_2763 0.10 -1.7 3,268,671 + Shew_2763 0.49 -3.5
Or see this region's nucleotide sequence