Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3997 and Dshi_3998 overlap by 4 nucleotides Dshi_3998 and Dshi_3999 are separated by 80 nucleotides Dshi_3999 and Dshi_4000 are separated by 124 nucleotides
Dshi_3997: Dshi_3997 - hypothetical protein (RefSeq), at 58,778 to 60,277
_3997
Dshi_3998: Dshi_3998 - DNA polymerase III, alpha subunit (RefSeq), at 60,274 to 63,624
_3998
Dshi_3999: Dshi_3999 - ParB-like nuclease (RefSeq), at 63,705 to 64,433
_3999
Dshi_4000: Dshi_4000 - hypothetical protein (RefSeq), at 64,558 to 64,860
_4000
Position (kb) 60
61
62
63
64 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 59.458 kb on - strand, within Dshi_3997 at 59.460 kb on + strand, within Dshi_3997 at 59.502 kb on + strand, within Dshi_3997 at 59.571 kb on - strand, within Dshi_3997 at 59.655 kb on - strand, within Dshi_3997 at 60.219 kb on - strand at 60.247 kb on + strand at 60.485 kb on + strand at 60.505 kb on - strand at 60.614 kb on - strand, within Dshi_3998 at 60.683 kb on + strand, within Dshi_3998 at 60.696 kb on - strand, within Dshi_3998 at 60.707 kb on - strand, within Dshi_3998 at 60.946 kb on + strand, within Dshi_3998 at 61.001 kb on + strand, within Dshi_3998 at 61.024 kb on + strand, within Dshi_3998 at 61.028 kb on + strand, within Dshi_3998 at 61.276 kb on + strand, within Dshi_3998 at 61.410 kb on + strand, within Dshi_3998 at 61.471 kb on - strand, within Dshi_3998 at 61.488 kb on + strand, within Dshi_3998 at 61.546 kb on - strand, within Dshi_3998 at 61.699 kb on + strand, within Dshi_3998 at 61.914 kb on + strand, within Dshi_3998 at 61.922 kb on - strand, within Dshi_3998 at 61.985 kb on + strand, within Dshi_3998 at 62.116 kb on + strand, within Dshi_3998 at 62.127 kb on + strand, within Dshi_3998 at 62.161 kb on + strand, within Dshi_3998 at 62.352 kb on + strand, within Dshi_3998 at 62.362 kb on - strand, within Dshi_3998 at 62.676 kb on + strand, within Dshi_3998 at 62.697 kb on - strand, within Dshi_3998 at 62.776 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on - strand, within Dshi_3998 at 62.797 kb on - strand, within Dshi_3998 at 62.797 kb on - strand, within Dshi_3998 at 62.901 kb on + strand, within Dshi_3998 at 63.193 kb on - strand, within Dshi_3998 at 63.276 kb on + strand, within Dshi_3998 at 63.343 kb on + strand at 63.396 kb on + strand at 63.534 kb on + strand at 63.565 kb on + strand at 63.581 kb on - strand at 63.788 kb on - strand, within Dshi_3999 at 63.797 kb on + strand, within Dshi_3999 at 63.800 kb on - strand, within Dshi_3999 at 63.805 kb on - strand, within Dshi_3999 at 63.840 kb on - strand, within Dshi_3999 at 63.921 kb on + strand, within Dshi_3999 at 64.131 kb on + strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.158 kb on - strand, within Dshi_3999 at 64.173 kb on - strand, within Dshi_3999 at 64.192 kb on + strand, within Dshi_3999 at 64.235 kb on - strand, within Dshi_3999 at 64.252 kb on + strand, within Dshi_3999 at 64.276 kb on + strand, within Dshi_3999 at 64.341 kb on - strand, within Dshi_3999 at 64.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 59,458 - Dshi_3997 0.45 -0.7 59,460 + Dshi_3997 0.45 -1.7 59,502 + Dshi_3997 0.48 -1.9 59,571 - Dshi_3997 0.53 -0.6 59,655 - Dshi_3997 0.58 +1.2 60,219 - -2.1 60,247 + -0.3 60,485 + -0.1 60,505 - -0.2 60,614 - Dshi_3998 0.10 +0.9 60,683 + Dshi_3998 0.12 +0.1 60,696 - Dshi_3998 0.13 -0.4 60,707 - Dshi_3998 0.13 -0.3 60,946 + Dshi_3998 0.20 +1.1 61,001 + Dshi_3998 0.22 -1.3 61,024 + Dshi_3998 0.22 +0.5 61,028 + Dshi_3998 0.23 -0.9 61,276 + Dshi_3998 0.30 +0.1 61,410 + Dshi_3998 0.34 +0.3 61,471 - Dshi_3998 0.36 -1.0 61,488 + Dshi_3998 0.36 +2.8 61,546 - Dshi_3998 0.38 -1.7 61,699 + Dshi_3998 0.43 -1.0 61,914 + Dshi_3998 0.49 -0.8 61,922 - Dshi_3998 0.49 -0.3 61,985 + Dshi_3998 0.51 -0.2 62,116 + Dshi_3998 0.55 +0.1 62,127 + Dshi_3998 0.55 -2.6 62,161 + Dshi_3998 0.56 +0.1 62,352 + Dshi_3998 0.62 +0.3 62,362 - Dshi_3998 0.62 +2.9 62,676 + Dshi_3998 0.72 +0.2 62,697 - Dshi_3998 0.72 -3.9 62,776 + Dshi_3998 0.75 -0.7 62,789 + Dshi_3998 0.75 -0.1 62,789 + Dshi_3998 0.75 -1.2 62,789 + Dshi_3998 0.75 +0.2 62,789 + Dshi_3998 0.75 -0.2 62,789 + Dshi_3998 0.75 +0.3 62,789 - Dshi_3998 0.75 -2.4 62,797 - Dshi_3998 0.75 -0.6 62,797 - Dshi_3998 0.75 -1.1 62,901 + Dshi_3998 0.78 -1.9 63,193 - Dshi_3998 0.87 -1.1 63,276 + Dshi_3998 0.90 -1.5 63,343 + -2.9 63,396 + -0.3 63,534 + -3.2 63,565 + -0.1 63,581 - -3.0 63,788 - Dshi_3999 0.11 -2.3 63,797 + Dshi_3999 0.13 -0.4 63,800 - Dshi_3999 0.13 +0.7 63,805 - Dshi_3999 0.14 +0.4 63,840 - Dshi_3999 0.19 -0.7 63,921 + Dshi_3999 0.30 +0.3 64,131 + Dshi_3999 0.58 +0.2 64,139 - Dshi_3999 0.60 -1.5 64,139 - Dshi_3999 0.60 -0.4 64,139 - Dshi_3999 0.60 +0.3 64,139 - Dshi_3999 0.60 -0.5 64,139 - Dshi_3999 0.60 -0.8 64,158 - Dshi_3999 0.62 +0.4 64,173 - Dshi_3999 0.64 -0.5 64,192 + Dshi_3999 0.67 +1.5 64,235 - Dshi_3999 0.73 -0.7 64,252 + Dshi_3999 0.75 -0.5 64,276 + Dshi_3999 0.78 +0.2 64,341 - Dshi_3999 0.87 -0.7 64,420 - -1.7
Or see this region's nucleotide sequence