Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3995 and Dshi_3996 overlap by 11 nucleotides Dshi_3996 and Dshi_3997 overlap by 74 nucleotides Dshi_3997 and Dshi_3998 overlap by 4 nucleotides
Dshi_3995: Dshi_3995 - protein of unknown function DUF159 (RefSeq), at 57,594 to 58,274
_3995
Dshi_3996: Dshi_3996 - hypothetical protein (RefSeq), at 58,264 to 58,851
_3996
Dshi_3997: Dshi_3997 - hypothetical protein (RefSeq), at 58,778 to 60,277
_3997
Dshi_3998: Dshi_3998 - DNA polymerase III, alpha subunit (RefSeq), at 60,274 to 63,624
_3998
Position (kb)
58
59
60
61 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 57.810 kb on - strand, within Dshi_3995 at 57.897 kb on - strand, within Dshi_3995 at 57.968 kb on + strand, within Dshi_3995 at 58.034 kb on - strand, within Dshi_3995 at 58.034 kb on - strand, within Dshi_3995 at 58.091 kb on - strand, within Dshi_3995 at 58.131 kb on + strand, within Dshi_3995 at 58.132 kb on - strand, within Dshi_3995 at 58.162 kb on - strand, within Dshi_3995 at 58.162 kb on - strand, within Dshi_3995 at 58.162 kb on - strand, within Dshi_3995 at 58.338 kb on - strand, within Dshi_3996 at 58.356 kb on + strand, within Dshi_3996 at 58.356 kb on + strand, within Dshi_3996 at 58.395 kb on - strand, within Dshi_3996 at 58.470 kb on - strand, within Dshi_3996 at 58.475 kb on - strand, within Dshi_3996 at 58.494 kb on + strand, within Dshi_3996 at 58.494 kb on + strand, within Dshi_3996 at 58.502 kb on - strand, within Dshi_3996 at 58.502 kb on - strand, within Dshi_3996 at 58.537 kb on - strand, within Dshi_3996 at 58.635 kb on - strand, within Dshi_3996 at 58.650 kb on - strand, within Dshi_3996 at 58.669 kb on + strand, within Dshi_3996 at 58.847 kb on + strand at 58.892 kb on + strand at 58.966 kb on + strand, within Dshi_3997 at 58.971 kb on + strand, within Dshi_3997 at 58.971 kb on + strand, within Dshi_3997 at 59.074 kb on - strand, within Dshi_3997 at 59.224 kb on - strand, within Dshi_3997 at 59.458 kb on - strand, within Dshi_3997 at 59.460 kb on + strand, within Dshi_3997 at 59.502 kb on + strand, within Dshi_3997 at 59.571 kb on - strand, within Dshi_3997 at 59.655 kb on - strand, within Dshi_3997 at 60.219 kb on - strand at 60.247 kb on + strand at 60.485 kb on + strand at 60.505 kb on - strand at 60.614 kb on - strand, within Dshi_3998 at 60.683 kb on + strand, within Dshi_3998 at 60.696 kb on - strand, within Dshi_3998 at 60.707 kb on - strand, within Dshi_3998 at 60.946 kb on + strand, within Dshi_3998 at 61.001 kb on + strand, within Dshi_3998 at 61.024 kb on + strand, within Dshi_3998 at 61.028 kb on + strand, within Dshi_3998 at 61.276 kb on + strand, within Dshi_3998
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 57,810 - Dshi_3995 0.32 -1.1 57,897 - Dshi_3995 0.44 -0.7 57,968 + Dshi_3995 0.55 -0.2 58,034 - Dshi_3995 0.65 -0.8 58,034 - Dshi_3995 0.65 -0.0 58,091 - Dshi_3995 0.73 -4.6 58,131 + Dshi_3995 0.79 -0.3 58,132 - Dshi_3995 0.79 -1.8 58,162 - Dshi_3995 0.83 -0.8 58,162 - Dshi_3995 0.83 -0.8 58,162 - Dshi_3995 0.83 -1.3 58,338 - Dshi_3996 0.13 +0.4 58,356 + Dshi_3996 0.16 +1.7 58,356 + Dshi_3996 0.16 +2.3 58,395 - Dshi_3996 0.22 +0.6 58,470 - Dshi_3996 0.35 +0.3 58,475 - Dshi_3996 0.36 -0.5 58,494 + Dshi_3996 0.39 +3.1 58,494 + Dshi_3996 0.39 +1.1 58,502 - Dshi_3996 0.40 +4.1 58,502 - Dshi_3996 0.40 -0.3 58,537 - Dshi_3996 0.46 -0.6 58,635 - Dshi_3996 0.63 -1.4 58,650 - Dshi_3996 0.66 +1.0 58,669 + Dshi_3996 0.69 +0.3 58,847 + -0.2 58,892 + -1.1 58,966 + Dshi_3997 0.13 +0.0 58,971 + Dshi_3997 0.13 +0.7 58,971 + Dshi_3997 0.13 -1.4 59,074 - Dshi_3997 0.20 -0.0 59,224 - Dshi_3997 0.30 +0.2 59,458 - Dshi_3997 0.45 -0.7 59,460 + Dshi_3997 0.45 -1.7 59,502 + Dshi_3997 0.48 -1.9 59,571 - Dshi_3997 0.53 -0.6 59,655 - Dshi_3997 0.58 +1.2 60,219 - -2.1 60,247 + -0.3 60,485 + -0.1 60,505 - -0.2 60,614 - Dshi_3998 0.10 +0.9 60,683 + Dshi_3998 0.12 +0.1 60,696 - Dshi_3998 0.13 -0.4 60,707 - Dshi_3998 0.13 -0.3 60,946 + Dshi_3998 0.20 +1.1 61,001 + Dshi_3998 0.22 -1.3 61,024 + Dshi_3998 0.22 +0.5 61,028 + Dshi_3998 0.23 -0.9 61,276 + Dshi_3998 0.30 +0.1
Or see this region's nucleotide sequence