Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3979 and Dshi_3980 are separated by 16 nucleotides Dshi_3980 and Dshi_3981 are separated by 2 nucleotides Dshi_3981 and Dshi_3982 overlap by 4 nucleotides
Dshi_3979: Dshi_3979 - hypothetical protein (RefSeq), at 38,371 to 39,180
_3979
Dshi_3980: Dshi_3980 - VirB8 family protein (RefSeq), at 39,197 to 39,850
_3980
Dshi_3981: Dshi_3981 - Conjugal transfer protein TrbG/VirB9/CagX (RefSeq), at 39,853 to 40,548
_3981
Dshi_3982: Dshi_3982 - conjugation TrbI family protein (RefSeq), at 40,545 to 41,924
_3982
Position (kb) 39
40
41 Strain fitness (log2 ratio) -2
-1
0
1
2 at 38.883 kb on + strand, within Dshi_3979 at 38.883 kb on + strand, within Dshi_3979 at 38.883 kb on + strand, within Dshi_3979 at 38.891 kb on - strand, within Dshi_3979 at 38.932 kb on - strand, within Dshi_3979 at 38.951 kb on + strand, within Dshi_3979 at 39.107 kb on - strand at 39.165 kb on + strand at 39.180 kb on + strand at 39.192 kb on + strand at 39.357 kb on + strand, within Dshi_3980 at 39.366 kb on + strand, within Dshi_3980 at 39.366 kb on + strand, within Dshi_3980 at 39.384 kb on + strand, within Dshi_3980 at 39.392 kb on - strand, within Dshi_3980 at 39.392 kb on - strand, within Dshi_3980 at 39.394 kb on + strand, within Dshi_3980 at 39.437 kb on - strand, within Dshi_3980 at 39.463 kb on - strand, within Dshi_3980 at 39.474 kb on + strand, within Dshi_3980 at 39.529 kb on - strand, within Dshi_3980 at 39.531 kb on + strand, within Dshi_3980 at 39.574 kb on - strand, within Dshi_3980 at 39.689 kb on + strand, within Dshi_3980 at 39.715 kb on + strand, within Dshi_3980 at 39.721 kb on + strand, within Dshi_3980 at 39.724 kb on + strand, within Dshi_3980 at 39.732 kb on - strand, within Dshi_3980 at 39.782 kb on + strand, within Dshi_3980 at 39.786 kb on + strand at 39.789 kb on - strand at 39.790 kb on - strand at 39.790 kb on - strand at 39.801 kb on + strand at 39.801 kb on + strand at 39.803 kb on - strand at 39.809 kb on - strand at 39.844 kb on + strand at 39.889 kb on - strand at 39.912 kb on - strand at 39.962 kb on + strand, within Dshi_3981 at 39.962 kb on + strand, within Dshi_3981 at 39.962 kb on + strand, within Dshi_3981 at 39.962 kb on + strand, within Dshi_3981 at 39.970 kb on - strand, within Dshi_3981 at 39.973 kb on - strand, within Dshi_3981 at 39.981 kb on - strand, within Dshi_3981 at 40.000 kb on - strand, within Dshi_3981 at 40.072 kb on - strand, within Dshi_3981 at 40.073 kb on + strand, within Dshi_3981 at 40.118 kb on + strand, within Dshi_3981 at 40.161 kb on - strand, within Dshi_3981 at 40.268 kb on - strand, within Dshi_3981 at 40.322 kb on - strand, within Dshi_3981 at 40.802 kb on + strand, within Dshi_3982 at 40.802 kb on + strand, within Dshi_3982 at 40.822 kb on + strand, within Dshi_3982 at 40.843 kb on + strand, within Dshi_3982 at 40.883 kb on + strand, within Dshi_3982
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 38,883 + Dshi_3979 0.63 -0.6 38,883 + Dshi_3979 0.63 +1.3 38,883 + Dshi_3979 0.63 +1.0 38,891 - Dshi_3979 0.64 -0.7 38,932 - Dshi_3979 0.69 -1.2 38,951 + Dshi_3979 0.72 -0.6 39,107 - +1.1 39,165 + -1.2 39,180 + +0.3 39,192 + -0.4 39,357 + Dshi_3980 0.24 -2.0 39,366 + Dshi_3980 0.26 -0.9 39,366 + Dshi_3980 0.26 -0.6 39,384 + Dshi_3980 0.29 -0.3 39,392 - Dshi_3980 0.30 -0.4 39,392 - Dshi_3980 0.30 +0.6 39,394 + Dshi_3980 0.30 +0.4 39,437 - Dshi_3980 0.37 +0.2 39,463 - Dshi_3980 0.41 +1.7 39,474 + Dshi_3980 0.42 +0.1 39,529 - Dshi_3980 0.51 +0.7 39,531 + Dshi_3980 0.51 -0.8 39,574 - Dshi_3980 0.58 -0.2 39,689 + Dshi_3980 0.75 +1.3 39,715 + Dshi_3980 0.79 +0.2 39,721 + Dshi_3980 0.80 -1.0 39,724 + Dshi_3980 0.81 -0.2 39,732 - Dshi_3980 0.82 +1.9 39,782 + Dshi_3980 0.89 -0.6 39,786 + -0.4 39,789 - +0.8 39,790 - +0.3 39,790 - -0.1 39,801 + -0.2 39,801 + +0.3 39,803 - -0.4 39,809 - -0.9 39,844 + +0.4 39,889 - -2.0 39,912 - -1.1 39,962 + Dshi_3981 0.16 +0.3 39,962 + Dshi_3981 0.16 -1.3 39,962 + Dshi_3981 0.16 -0.7 39,962 + Dshi_3981 0.16 -1.1 39,970 - Dshi_3981 0.17 -1.6 39,973 - Dshi_3981 0.17 +0.2 39,981 - Dshi_3981 0.18 +1.1 40,000 - Dshi_3981 0.21 -0.8 40,072 - Dshi_3981 0.31 +0.0 40,073 + Dshi_3981 0.32 -1.4 40,118 + Dshi_3981 0.38 -0.5 40,161 - Dshi_3981 0.44 -0.5 40,268 - Dshi_3981 0.60 -0.5 40,322 - Dshi_3981 0.67 +1.0 40,802 + Dshi_3982 0.19 +2.0 40,802 + Dshi_3982 0.19 -0.3 40,822 + Dshi_3982 0.20 +0.9 40,843 + Dshi_3982 0.22 -1.1 40,883 + Dshi_3982 0.24 +2.5
Or see this region's nucleotide sequence