Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3824 and Dshi_3825 are separated by 22 nucleotides Dshi_3825 and Dshi_3826 are separated by 212 nucleotides Dshi_3826 and Dshi_3827 are separated by 41 nucleotides
Dshi_3824: Dshi_3824 - phenylacetate-CoA ligase (RefSeq), at 30,459 to 31,769
_3824
Dshi_3825: Dshi_3825 - phenylacetic acid degradation protein PaaD (RefSeq), at 31,792 to 32,217
_3825
Dshi_3826: Dshi_3826 - 3-hydroxyacyl-CoA dehydrogenase NAD-binding (RefSeq), at 32,430 to 34,451
_3826
Dshi_3827: Dshi_3827 - phenylacetic acid degradation protein paaN (RefSeq), at 34,493 to 36,529
_3827
Position (kb) 32
33
34
35 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 31.446 kb on + strand, within Dshi_3824 at 31.471 kb on - strand, within Dshi_3824 at 31.482 kb on + strand, within Dshi_3824 at 31.501 kb on - strand, within Dshi_3824 at 31.698 kb on - strand at 31.747 kb on - strand at 31.773 kb on + strand at 31.784 kb on + strand at 31.810 kb on + strand at 31.858 kb on + strand, within Dshi_3825 at 31.944 kb on + strand, within Dshi_3825 at 31.995 kb on + strand, within Dshi_3825 at 32.083 kb on - strand, within Dshi_3825 at 32.094 kb on + strand, within Dshi_3825 at 32.094 kb on + strand, within Dshi_3825 at 32.094 kb on + strand, within Dshi_3825 at 32.102 kb on - strand, within Dshi_3825 at 32.148 kb on + strand, within Dshi_3825 at 32.308 kb on + strand at 32.704 kb on + strand, within Dshi_3826 at 32.721 kb on + strand, within Dshi_3826 at 33.061 kb on + strand, within Dshi_3826 at 33.124 kb on - strand, within Dshi_3826 at 33.181 kb on - strand, within Dshi_3826 at 33.257 kb on + strand, within Dshi_3826 at 33.291 kb on + strand, within Dshi_3826 at 33.342 kb on - strand, within Dshi_3826 at 33.345 kb on + strand, within Dshi_3826 at 33.472 kb on + strand, within Dshi_3826 at 33.477 kb on + strand, within Dshi_3826 at 33.478 kb on - strand, within Dshi_3826 at 33.515 kb on + strand, within Dshi_3826 at 33.666 kb on + strand, within Dshi_3826 at 33.994 kb on - strand, within Dshi_3826 at 34.047 kb on + strand, within Dshi_3826 at 34.196 kb on - strand, within Dshi_3826 at 34.212 kb on + strand, within Dshi_3826 at 34.212 kb on + strand, within Dshi_3826 at 34.354 kb on + strand at 34.491 kb on + strand at 34.535 kb on - strand at 34.656 kb on + strand at 34.712 kb on + strand, within Dshi_3827 at 34.756 kb on - strand, within Dshi_3827 at 34.817 kb on + strand, within Dshi_3827 at 34.817 kb on + strand, within Dshi_3827 at 34.825 kb on - strand, within Dshi_3827 at 34.825 kb on - strand, within Dshi_3827 at 34.929 kb on - strand, within Dshi_3827 at 34.986 kb on - strand, within Dshi_3827 at 35.169 kb on + strand, within Dshi_3827 at 35.169 kb on + strand, within Dshi_3827 at 35.306 kb on + strand, within Dshi_3827
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 31,446 + Dshi_3824 0.75 -0.4 31,471 - Dshi_3824 0.77 -1.3 31,482 + Dshi_3824 0.78 -0.6 31,501 - Dshi_3824 0.79 -1.9 31,698 - +0.6 31,747 - -0.3 31,773 + +0.4 31,784 + -1.7 31,810 + +2.3 31,858 + Dshi_3825 0.15 +1.0 31,944 + Dshi_3825 0.36 +1.0 31,995 + Dshi_3825 0.48 +1.0 32,083 - Dshi_3825 0.68 -2.9 32,094 + Dshi_3825 0.71 +0.5 32,094 + Dshi_3825 0.71 +1.7 32,094 + Dshi_3825 0.71 -0.0 32,102 - Dshi_3825 0.73 +3.0 32,148 + Dshi_3825 0.84 +3.3 32,308 + +4.9 32,704 + Dshi_3826 0.14 -0.0 32,721 + Dshi_3826 0.14 +1.0 33,061 + Dshi_3826 0.31 +3.3 33,124 - Dshi_3826 0.34 -1.4 33,181 - Dshi_3826 0.37 +2.0 33,257 + Dshi_3826 0.41 -0.0 33,291 + Dshi_3826 0.43 +1.0 33,342 - Dshi_3826 0.45 +3.0 33,345 + Dshi_3826 0.45 +1.0 33,472 + Dshi_3826 0.52 +1.0 33,477 + Dshi_3826 0.52 +0.4 33,478 - Dshi_3826 0.52 +2.5 33,515 + Dshi_3826 0.54 +3.3 33,666 + Dshi_3826 0.61 +3.5 33,994 - Dshi_3826 0.77 +1.0 34,047 + Dshi_3826 0.80 -0.0 34,196 - Dshi_3826 0.87 -1.0 34,212 + Dshi_3826 0.88 +2.5 34,212 + Dshi_3826 0.88 -2.6 34,354 + +2.4 34,491 + +2.5 34,535 - -2.3 34,656 + +2.0 34,712 + Dshi_3827 0.11 +0.6 34,756 - Dshi_3827 0.13 +2.0 34,817 + Dshi_3827 0.16 +1.4 34,817 + Dshi_3827 0.16 +1.0 34,825 - Dshi_3827 0.16 -0.5 34,825 - Dshi_3827 0.16 +3.0 34,929 - Dshi_3827 0.21 -0.6 34,986 - Dshi_3827 0.24 -1.2 35,169 + Dshi_3827 0.33 -0.1 35,169 + Dshi_3827 0.33 +0.2 35,306 + Dshi_3827 0.40 -1.6
Or see this region's nucleotide sequence