Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3434 and Dshi_3435 overlap by 4 nucleotides Dshi_3435 and Dshi_3436 overlap by 16 nucleotides Dshi_3436 and Dshi_3437 overlap by 4 nucleotides
Dshi_3434: Dshi_3434 - protein of unknown function UPF0079 (RefSeq), at 3,615,034 to 3,615,531
_3434
Dshi_3435: Dshi_3435 - aminoglycoside phosphotransferase (RefSeq), at 3,615,528 to 3,616,526
_3435
Dshi_3436: Dshi_3436 - Nucleotidyl transferase (RefSeq), at 3,616,511 to 3,617,194
_3436
Dshi_3437: Dshi_3437 - Double-strand break repair protein AddB (RefSeq), at 3,617,191 to 3,620,157
_3437
Position (kb)
3616
3617
3618 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3615.520 kb on - strand at 3615.520 kb on - strand at 3615.525 kb on - strand at 3615.572 kb on - strand at 3615.622 kb on + strand at 3615.665 kb on - strand, within Dshi_3435 at 3615.677 kb on + strand, within Dshi_3435 at 3615.722 kb on + strand, within Dshi_3435 at 3615.722 kb on - strand, within Dshi_3435 at 3615.835 kb on + strand, within Dshi_3435 at 3615.843 kb on - strand, within Dshi_3435 at 3615.843 kb on - strand, within Dshi_3435 at 3615.864 kb on - strand, within Dshi_3435 at 3615.878 kb on + strand, within Dshi_3435 at 3615.893 kb on + strand, within Dshi_3435 at 3615.896 kb on - strand, within Dshi_3435 at 3615.907 kb on - strand, within Dshi_3435 at 3615.937 kb on - strand, within Dshi_3435 at 3616.001 kb on - strand, within Dshi_3435 at 3616.039 kb on + strand, within Dshi_3435 at 3616.088 kb on - strand, within Dshi_3435 at 3616.101 kb on - strand, within Dshi_3435 at 3616.124 kb on + strand, within Dshi_3435 at 3616.154 kb on - strand, within Dshi_3435 at 3616.159 kb on - strand, within Dshi_3435 at 3616.215 kb on - strand, within Dshi_3435 at 3616.238 kb on + strand, within Dshi_3435 at 3616.244 kb on - strand, within Dshi_3435 at 3616.269 kb on + strand, within Dshi_3435 at 3616.280 kb on + strand, within Dshi_3435 at 3616.288 kb on - strand, within Dshi_3435 at 3616.393 kb on - strand, within Dshi_3435 at 3616.411 kb on - strand, within Dshi_3435 at 3616.425 kb on - strand, within Dshi_3435 at 3616.430 kb on - strand at 3616.440 kb on + strand at 3616.479 kb on - strand at 3616.507 kb on + strand at 3616.531 kb on - strand at 3616.587 kb on - strand, within Dshi_3436 at 3616.636 kb on + strand, within Dshi_3436 at 3616.679 kb on - strand, within Dshi_3436 at 3616.716 kb on + strand, within Dshi_3436 at 3616.724 kb on - strand, within Dshi_3436 at 3616.774 kb on + strand, within Dshi_3436 at 3616.781 kb on + strand, within Dshi_3436 at 3616.781 kb on + strand, within Dshi_3436 at 3616.782 kb on - strand, within Dshi_3436 at 3616.782 kb on - strand, within Dshi_3436 at 3616.800 kb on - strand, within Dshi_3436 at 3616.831 kb on + strand, within Dshi_3436 at 3616.831 kb on + strand, within Dshi_3436 at 3616.851 kb on + strand, within Dshi_3436 at 3616.869 kb on - strand, within Dshi_3436 at 3616.869 kb on - strand, within Dshi_3436 at 3616.869 kb on - strand, within Dshi_3436 at 3616.930 kb on + strand, within Dshi_3436 at 3616.930 kb on + strand, within Dshi_3436 at 3616.938 kb on - strand, within Dshi_3436 at 3616.938 kb on - strand, within Dshi_3436 at 3616.938 kb on - strand, within Dshi_3436 at 3616.970 kb on - strand, within Dshi_3436 at 3616.986 kb on - strand, within Dshi_3436 at 3617.004 kb on - strand, within Dshi_3436 at 3617.004 kb on - strand, within Dshi_3436 at 3617.033 kb on - strand, within Dshi_3436 at 3617.061 kb on + strand, within Dshi_3436 at 3617.080 kb on + strand, within Dshi_3436 at 3617.088 kb on + strand, within Dshi_3436 at 3617.088 kb on + strand, within Dshi_3436 at 3617.090 kb on + strand, within Dshi_3436 at 3617.122 kb on - strand, within Dshi_3436 at 3617.155 kb on - strand at 3617.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 3,615,520 - +1.9 3,615,520 - +0.0 3,615,525 - -2.3 3,615,572 - -0.6 3,615,622 + -3.5 3,615,665 - Dshi_3435 0.14 +0.6 3,615,677 + Dshi_3435 0.15 -0.3 3,615,722 + Dshi_3435 0.19 -0.2 3,615,722 - Dshi_3435 0.19 -1.8 3,615,835 + Dshi_3435 0.31 -3.3 3,615,843 - Dshi_3435 0.32 -1.8 3,615,843 - Dshi_3435 0.32 +0.5 3,615,864 - Dshi_3435 0.34 -0.7 3,615,878 + Dshi_3435 0.35 +0.8 3,615,893 + Dshi_3435 0.37 +2.2 3,615,896 - Dshi_3435 0.37 +1.0 3,615,907 - Dshi_3435 0.38 +0.5 3,615,937 - Dshi_3435 0.41 -0.0 3,616,001 - Dshi_3435 0.47 +0.4 3,616,039 + Dshi_3435 0.51 -0.2 3,616,088 - Dshi_3435 0.56 +1.7 3,616,101 - Dshi_3435 0.57 -1.5 3,616,124 + Dshi_3435 0.60 +0.1 3,616,154 - Dshi_3435 0.63 +0.2 3,616,159 - Dshi_3435 0.63 -0.1 3,616,215 - Dshi_3435 0.69 +0.1 3,616,238 + Dshi_3435 0.71 -0.6 3,616,244 - Dshi_3435 0.72 -1.0 3,616,269 + Dshi_3435 0.74 +1.1 3,616,280 + Dshi_3435 0.75 +0.7 3,616,288 - Dshi_3435 0.76 +4.1 3,616,393 - Dshi_3435 0.87 -1.6 3,616,411 - Dshi_3435 0.88 +0.3 3,616,425 - Dshi_3435 0.90 -0.4 3,616,430 - -0.6 3,616,440 + +1.0 3,616,479 - +0.5 3,616,507 + +0.2 3,616,531 - +1.7 3,616,587 - Dshi_3436 0.11 +1.0 3,616,636 + Dshi_3436 0.18 +1.1 3,616,679 - Dshi_3436 0.25 -2.9 3,616,716 + Dshi_3436 0.30 +0.3 3,616,724 - Dshi_3436 0.31 +1.7 3,616,774 + Dshi_3436 0.38 +1.7 3,616,781 + Dshi_3436 0.39 -0.5 3,616,781 + Dshi_3436 0.39 -0.5 3,616,782 - Dshi_3436 0.40 +1.3 3,616,782 - Dshi_3436 0.40 +1.9 3,616,800 - Dshi_3436 0.42 +0.9 3,616,831 + Dshi_3436 0.47 +0.2 3,616,831 + Dshi_3436 0.47 +0.4 3,616,851 + Dshi_3436 0.50 +3.4 3,616,869 - Dshi_3436 0.52 +0.2 3,616,869 - Dshi_3436 0.52 -0.9 3,616,869 - Dshi_3436 0.52 +2.1 3,616,930 + Dshi_3436 0.61 -1.7 3,616,930 + Dshi_3436 0.61 -0.1 3,616,938 - Dshi_3436 0.62 +0.7 3,616,938 - Dshi_3436 0.62 +0.3 3,616,938 - Dshi_3436 0.62 -2.7 3,616,970 - Dshi_3436 0.67 +1.0 3,616,986 - Dshi_3436 0.69 +1.0 3,617,004 - Dshi_3436 0.72 +2.6 3,617,004 - Dshi_3436 0.72 +0.5 3,617,033 - Dshi_3436 0.76 -0.2 3,617,061 + Dshi_3436 0.80 +0.9 3,617,080 + Dshi_3436 0.83 +0.9 3,617,088 + Dshi_3436 0.84 +0.9 3,617,088 + Dshi_3436 0.84 +0.4 3,617,090 + Dshi_3436 0.85 -0.5 3,617,122 - Dshi_3436 0.89 +0.4 3,617,155 - +2.0 3,617,155 - -0.2
Or see this region's nucleotide sequence