Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3311 and Dshi_3312 are separated by 146 nucleotides Dshi_3312 and Dshi_3313 overlap by 4 nucleotides Dshi_3313 and Dshi_3314 are separated by 445 nucleotides
Dshi_3311: Dshi_3311 - radical SAM enzyme, Cfr family (RefSeq), at 3,474,155 to 3,475,375
_3311
Dshi_3312: Dshi_3312 - hypothetical protein (RefSeq), at 3,475,522 to 3,476,199
_3312
Dshi_3313: Dshi_3313 - hypothetical protein (RefSeq), at 3,476,196 to 3,476,906
_3313
Dshi_3314: Dshi_3314 - hypothetical protein (RefSeq), at 3,477,352 to 3,477,885
_3314
Position (kb)
3476
3477 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3475.245 kb on + strand, within Dshi_3311 at 3475.250 kb on + strand, within Dshi_3311 at 3475.250 kb on + strand at 3475.258 kb on - strand at 3475.258 kb on - strand at 3475.282 kb on - strand at 3475.290 kb on - strand at 3475.301 kb on + strand at 3475.305 kb on + strand at 3475.345 kb on - strand at 3475.349 kb on + strand at 3475.373 kb on - strand at 3475.373 kb on - strand at 3475.494 kb on - strand at 3475.496 kb on + strand at 3475.524 kb on + strand at 3475.524 kb on + strand at 3475.532 kb on - strand at 3475.532 kb on - strand at 3475.537 kb on + strand at 3475.545 kb on - strand at 3475.545 kb on - strand at 3475.622 kb on - strand, within Dshi_3312 at 3475.652 kb on + strand, within Dshi_3312 at 3475.660 kb on - strand, within Dshi_3312 at 3475.699 kb on - strand, within Dshi_3312 at 3475.699 kb on - strand, within Dshi_3312 at 3475.704 kb on + strand, within Dshi_3312 at 3475.734 kb on - strand, within Dshi_3312 at 3475.783 kb on - strand, within Dshi_3312 at 3475.838 kb on + strand, within Dshi_3312 at 3476.194 kb on + strand at 3476.245 kb on + strand at 3476.269 kb on - strand, within Dshi_3313 at 3476.277 kb on + strand, within Dshi_3313 at 3476.285 kb on - strand, within Dshi_3313 at 3476.319 kb on - strand, within Dshi_3313 at 3476.338 kb on - strand, within Dshi_3313 at 3476.481 kb on + strand, within Dshi_3313 at 3476.483 kb on - strand, within Dshi_3313 at 3476.489 kb on - strand, within Dshi_3313 at 3476.489 kb on - strand, within Dshi_3313 at 3476.489 kb on - strand, within Dshi_3313 at 3476.493 kb on + strand, within Dshi_3313 at 3476.591 kb on - strand, within Dshi_3313 at 3476.649 kb on - strand, within Dshi_3313 at 3476.781 kb on + strand, within Dshi_3313 at 3476.794 kb on - strand, within Dshi_3313 at 3476.941 kb on + strand at 3477.090 kb on + strand at 3477.098 kb on - strand at 3477.109 kb on + strand at 3477.109 kb on + strand at 3477.135 kb on - strand at 3477.166 kb on + strand at 3477.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 3,475,245 + Dshi_3311 0.89 -2.4 3,475,250 + Dshi_3311 0.90 -2.8 3,475,250 + +1.2 3,475,258 - -1.0 3,475,258 - +1.2 3,475,282 - -0.1 3,475,290 - +0.2 3,475,301 + -1.1 3,475,305 + +1.6 3,475,345 - -1.2 3,475,349 + -1.4 3,475,373 - -0.4 3,475,373 - -0.4 3,475,494 - -0.4 3,475,496 + -2.7 3,475,524 + +1.2 3,475,524 + +0.7 3,475,532 - +1.2 3,475,532 - -0.9 3,475,537 + -2.8 3,475,545 - +0.0 3,475,545 - +2.2 3,475,622 - Dshi_3312 0.15 +0.2 3,475,652 + Dshi_3312 0.19 +0.7 3,475,660 - Dshi_3312 0.20 -0.4 3,475,699 - Dshi_3312 0.26 -2.0 3,475,699 - Dshi_3312 0.26 +1.2 3,475,704 + Dshi_3312 0.27 +1.2 3,475,734 - Dshi_3312 0.31 +2.7 3,475,783 - Dshi_3312 0.38 +0.2 3,475,838 + Dshi_3312 0.47 +1.2 3,476,194 + +1.2 3,476,245 + -3.2 3,476,269 - Dshi_3313 0.10 +0.4 3,476,277 + Dshi_3313 0.11 -0.5 3,476,285 - Dshi_3313 0.13 +2.1 3,476,319 - Dshi_3313 0.17 -1.5 3,476,338 - Dshi_3313 0.20 -0.9 3,476,481 + Dshi_3313 0.40 -1.8 3,476,483 - Dshi_3313 0.40 +1.1 3,476,489 - Dshi_3313 0.41 -0.9 3,476,489 - Dshi_3313 0.41 -1.2 3,476,489 - Dshi_3313 0.41 -1.1 3,476,493 + Dshi_3313 0.42 +1.1 3,476,591 - Dshi_3313 0.56 -2.6 3,476,649 - Dshi_3313 0.64 -0.9 3,476,781 + Dshi_3313 0.82 +0.5 3,476,794 - Dshi_3313 0.84 +2.4 3,476,941 + -0.2 3,477,090 + +1.1 3,477,098 - +1.1 3,477,109 + +3.4 3,477,109 + -0.9 3,477,135 - -0.5 3,477,166 + +1.3 3,477,248 - -1.2
Or see this region's nucleotide sequence