Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3309 and Dshi_3310 are separated by 53 nucleotides Dshi_3310 and Dshi_3311 overlap by 1 nucleotides Dshi_3311 and Dshi_3312 are separated by 146 nucleotides Dshi_3312 and Dshi_3313 overlap by 4 nucleotides
Dshi_3309: Dshi_3309 - monofunctional biosynthetic peptidoglycan transglycosylase (RefSeq), at 3,472,552 to 3,473,298
_3309
Dshi_3310: Dshi_3310 - Patatin (RefSeq), at 3,473,352 to 3,474,155
_3310
Dshi_3311: Dshi_3311 - radical SAM enzyme, Cfr family (RefSeq), at 3,474,155 to 3,475,375
_3311
Dshi_3312: Dshi_3312 - hypothetical protein (RefSeq), at 3,475,522 to 3,476,199
_3312
Dshi_3313: Dshi_3313 - hypothetical protein (RefSeq), at 3,476,196 to 3,476,906
_3313
Position (kb)
3474
3475
3476 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3473.266 kb on - strand at 3473.282 kb on + strand at 3473.284 kb on + strand at 3473.312 kb on + strand at 3473.325 kb on - strand at 3473.340 kb on + strand at 3473.468 kb on - strand, within Dshi_3310 at 3473.550 kb on + strand, within Dshi_3310 at 3473.567 kb on - strand, within Dshi_3310 at 3473.593 kb on - strand, within Dshi_3310 at 3473.617 kb on + strand, within Dshi_3310 at 3473.808 kb on + strand, within Dshi_3310 at 3473.816 kb on - strand, within Dshi_3310 at 3473.916 kb on - strand, within Dshi_3310 at 3474.063 kb on - strand, within Dshi_3310 at 3474.283 kb on - strand, within Dshi_3311 at 3474.444 kb on + strand, within Dshi_3311 at 3474.452 kb on - strand, within Dshi_3311 at 3474.490 kb on + strand, within Dshi_3311 at 3474.500 kb on + strand, within Dshi_3311 at 3474.500 kb on + strand, within Dshi_3311 at 3474.526 kb on - strand, within Dshi_3311 at 3474.576 kb on - strand, within Dshi_3311 at 3474.576 kb on - strand, within Dshi_3311 at 3474.601 kb on + strand, within Dshi_3311 at 3474.654 kb on + strand, within Dshi_3311 at 3474.677 kb on + strand, within Dshi_3311 at 3474.727 kb on - strand, within Dshi_3311 at 3474.753 kb on + strand, within Dshi_3311 at 3474.817 kb on - strand, within Dshi_3311 at 3474.846 kb on + strand, within Dshi_3311 at 3474.874 kb on + strand, within Dshi_3311 at 3474.963 kb on + strand, within Dshi_3311 at 3474.997 kb on + strand, within Dshi_3311 at 3475.033 kb on - strand, within Dshi_3311 at 3475.113 kb on + strand, within Dshi_3311 at 3475.115 kb on - strand, within Dshi_3311 at 3475.143 kb on - strand, within Dshi_3311 at 3475.245 kb on + strand, within Dshi_3311 at 3475.250 kb on + strand, within Dshi_3311 at 3475.250 kb on + strand at 3475.258 kb on - strand at 3475.258 kb on - strand at 3475.282 kb on - strand at 3475.290 kb on - strand at 3475.301 kb on + strand at 3475.305 kb on + strand at 3475.345 kb on - strand at 3475.349 kb on + strand at 3475.373 kb on - strand at 3475.373 kb on - strand at 3475.494 kb on - strand at 3475.496 kb on + strand at 3475.524 kb on + strand at 3475.524 kb on + strand at 3475.532 kb on - strand at 3475.532 kb on - strand at 3475.537 kb on + strand at 3475.545 kb on - strand at 3475.545 kb on - strand at 3475.622 kb on - strand, within Dshi_3312 at 3475.652 kb on + strand, within Dshi_3312 at 3475.660 kb on - strand, within Dshi_3312 at 3475.699 kb on - strand, within Dshi_3312 at 3475.699 kb on - strand, within Dshi_3312 at 3475.704 kb on + strand, within Dshi_3312 at 3475.734 kb on - strand, within Dshi_3312 at 3475.783 kb on - strand, within Dshi_3312 at 3475.838 kb on + strand, within Dshi_3312 at 3476.194 kb on + strand at 3476.245 kb on + strand at 3476.269 kb on - strand, within Dshi_3313 at 3476.277 kb on + strand, within Dshi_3313 at 3476.285 kb on - strand, within Dshi_3313 at 3476.319 kb on - strand, within Dshi_3313 at 3476.338 kb on - strand, within Dshi_3313
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 3,473,266 - +1.6 3,473,282 + +0.7 3,473,284 + +3.2 3,473,312 + +0.2 3,473,325 - -0.4 3,473,340 + +0.8 3,473,468 - Dshi_3310 0.14 -0.1 3,473,550 + Dshi_3310 0.25 -1.2 3,473,567 - Dshi_3310 0.27 +1.2 3,473,593 - Dshi_3310 0.30 -1.8 3,473,617 + Dshi_3310 0.33 +0.5 3,473,808 + Dshi_3310 0.57 +1.2 3,473,816 - Dshi_3310 0.58 -2.4 3,473,916 - Dshi_3310 0.70 -2.3 3,474,063 - Dshi_3310 0.88 -2.9 3,474,283 - Dshi_3311 0.10 -1.4 3,474,444 + Dshi_3311 0.24 -0.5 3,474,452 - Dshi_3311 0.24 +0.3 3,474,490 + Dshi_3311 0.27 -0.4 3,474,500 + Dshi_3311 0.28 -0.8 3,474,500 + Dshi_3311 0.28 +1.2 3,474,526 - Dshi_3311 0.30 -0.6 3,474,576 - Dshi_3311 0.34 +1.2 3,474,576 - Dshi_3311 0.34 -1.4 3,474,601 + Dshi_3311 0.37 -1.4 3,474,654 + Dshi_3311 0.41 -2.7 3,474,677 + Dshi_3311 0.43 -1.2 3,474,727 - Dshi_3311 0.47 -3.3 3,474,753 + Dshi_3311 0.49 +1.2 3,474,817 - Dshi_3311 0.54 -0.5 3,474,846 + Dshi_3311 0.57 +1.2 3,474,874 + Dshi_3311 0.59 -1.8 3,474,963 + Dshi_3311 0.66 +0.1 3,474,997 + Dshi_3311 0.69 +1.2 3,475,033 - Dshi_3311 0.72 -1.6 3,475,113 + Dshi_3311 0.78 +3.7 3,475,115 - Dshi_3311 0.79 +1.2 3,475,143 - Dshi_3311 0.81 -1.8 3,475,245 + Dshi_3311 0.89 -2.4 3,475,250 + Dshi_3311 0.90 -2.8 3,475,250 + +1.2 3,475,258 - -1.0 3,475,258 - +1.2 3,475,282 - -0.1 3,475,290 - +0.2 3,475,301 + -1.1 3,475,305 + +1.6 3,475,345 - -1.2 3,475,349 + -1.4 3,475,373 - -0.4 3,475,373 - -0.4 3,475,494 - -0.4 3,475,496 + -2.7 3,475,524 + +1.2 3,475,524 + +0.7 3,475,532 - +1.2 3,475,532 - -0.9 3,475,537 + -2.8 3,475,545 - +0.0 3,475,545 - +2.2 3,475,622 - Dshi_3312 0.15 +0.2 3,475,652 + Dshi_3312 0.19 +0.7 3,475,660 - Dshi_3312 0.20 -0.4 3,475,699 - Dshi_3312 0.26 -2.0 3,475,699 - Dshi_3312 0.26 +1.2 3,475,704 + Dshi_3312 0.27 +1.2 3,475,734 - Dshi_3312 0.31 +2.7 3,475,783 - Dshi_3312 0.38 +0.2 3,475,838 + Dshi_3312 0.47 +1.2 3,476,194 + +1.2 3,476,245 + -3.2 3,476,269 - Dshi_3313 0.10 +0.4 3,476,277 + Dshi_3313 0.11 -0.5 3,476,285 - Dshi_3313 0.13 +2.1 3,476,319 - Dshi_3313 0.17 -1.5 3,476,338 - Dshi_3313 0.20 -0.9
Or see this region's nucleotide sequence