Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3253 and Dshi_3254 are separated by 12 nucleotides Dshi_3254 and Dshi_3255 are separated by 8 nucleotides Dshi_3255 and Dshi_3256 are separated by 376 nucleotides
Dshi_3253: Dshi_3253 - SAF domain protein (RefSeq), at 3,418,989 to 3,419,414
_3253
Dshi_3254: Dshi_3254 - flagellar L-ring protein (RefSeq), at 3,419,427 to 3,420,155
_3254
Dshi_3255: Dshi_3255 - flagellar basal body-associated protein FliL (RefSeq), at 3,420,164 to 3,420,655
_3255
Dshi_3256: Dshi_3256 - type III secretion exporter (RefSeq), at 3,421,032 to 3,422,120
_3256
Position (kb)
3420
3421 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3419.180 kb on + strand, within Dshi_3253 at 3419.188 kb on - strand, within Dshi_3253 at 3419.244 kb on + strand, within Dshi_3253 at 3419.271 kb on + strand, within Dshi_3253 at 3419.271 kb on - strand, within Dshi_3253 at 3419.301 kb on + strand, within Dshi_3253 at 3419.410 kb on + strand at 3419.410 kb on + strand at 3419.416 kb on - strand at 3419.463 kb on - strand at 3419.463 kb on - strand at 3419.503 kb on + strand, within Dshi_3254 at 3419.531 kb on - strand, within Dshi_3254 at 3419.544 kb on + strand, within Dshi_3254 at 3419.606 kb on + strand, within Dshi_3254 at 3419.608 kb on - strand, within Dshi_3254 at 3419.614 kb on - strand, within Dshi_3254 at 3419.626 kb on + strand, within Dshi_3254 at 3419.662 kb on + strand, within Dshi_3254 at 3419.748 kb on - strand, within Dshi_3254 at 3419.811 kb on + strand, within Dshi_3254 at 3419.886 kb on - strand, within Dshi_3254 at 3419.943 kb on + strand, within Dshi_3254 at 3419.953 kb on + strand, within Dshi_3254 at 3419.953 kb on + strand, within Dshi_3254 at 3419.971 kb on + strand, within Dshi_3254 at 3419.979 kb on - strand, within Dshi_3254 at 3420.022 kb on + strand, within Dshi_3254 at 3420.092 kb on + strand at 3420.100 kb on - strand at 3420.149 kb on + strand at 3420.149 kb on + strand at 3420.252 kb on + strand, within Dshi_3255 at 3420.396 kb on + strand, within Dshi_3255 at 3420.407 kb on + strand, within Dshi_3255 at 3420.417 kb on + strand, within Dshi_3255 at 3420.448 kb on + strand, within Dshi_3255 at 3420.456 kb on - strand, within Dshi_3255 at 3420.488 kb on - strand, within Dshi_3255 at 3420.543 kb on + strand, within Dshi_3255 at 3420.607 kb on + strand at 3420.678 kb on - strand at 3420.754 kb on + strand at 3420.759 kb on + strand at 3420.764 kb on + strand at 3420.764 kb on + strand at 3420.830 kb on - strand at 3420.910 kb on + strand at 3420.917 kb on + strand at 3420.931 kb on + strand at 3421.104 kb on - strand at 3421.242 kb on - strand, within Dshi_3256 at 3421.255 kb on - strand, within Dshi_3256 at 3421.256 kb on - strand, within Dshi_3256 at 3421.303 kb on + strand, within Dshi_3256 at 3421.436 kb on - strand, within Dshi_3256 at 3421.464 kb on + strand, within Dshi_3256 at 3421.471 kb on - strand, within Dshi_3256 at 3421.482 kb on + strand, within Dshi_3256 at 3421.626 kb on - strand, within Dshi_3256 at 3421.637 kb on + strand, within Dshi_3256
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 3,419,180 + Dshi_3253 0.45 -1.4 3,419,188 - Dshi_3253 0.47 +1.3 3,419,244 + Dshi_3253 0.60 -0.3 3,419,271 + Dshi_3253 0.66 -2.4 3,419,271 - Dshi_3253 0.66 +1.3 3,419,301 + Dshi_3253 0.73 -0.7 3,419,410 + +1.3 3,419,410 + -1.3 3,419,416 - +2.7 3,419,463 - -4.0 3,419,463 - -1.3 3,419,503 + Dshi_3254 0.10 +1.3 3,419,531 - Dshi_3254 0.14 -3.9 3,419,544 + Dshi_3254 0.16 +0.8 3,419,606 + Dshi_3254 0.25 +0.2 3,419,608 - Dshi_3254 0.25 +0.5 3,419,614 - Dshi_3254 0.26 +0.6 3,419,626 + Dshi_3254 0.27 +2.9 3,419,662 + Dshi_3254 0.32 +0.1 3,419,748 - Dshi_3254 0.44 -0.3 3,419,811 + Dshi_3254 0.53 -0.3 3,419,886 - Dshi_3254 0.63 -0.3 3,419,943 + Dshi_3254 0.71 -0.1 3,419,953 + Dshi_3254 0.72 +0.1 3,419,953 + Dshi_3254 0.72 +0.9 3,419,971 + Dshi_3254 0.75 +0.1 3,419,979 - Dshi_3254 0.76 -0.2 3,420,022 + Dshi_3254 0.82 +1.3 3,420,092 + +1.3 3,420,100 - -3.7 3,420,149 + -1.5 3,420,149 + +1.3 3,420,252 + Dshi_3255 0.18 +1.1 3,420,396 + Dshi_3255 0.47 -0.2 3,420,407 + Dshi_3255 0.49 +0.0 3,420,417 + Dshi_3255 0.51 -1.7 3,420,448 + Dshi_3255 0.58 -0.2 3,420,456 - Dshi_3255 0.59 +1.5 3,420,488 - Dshi_3255 0.66 +0.0 3,420,543 + Dshi_3255 0.77 +1.3 3,420,607 + -1.7 3,420,678 - -1.7 3,420,754 + +0.9 3,420,759 + -2.0 3,420,764 + -0.6 3,420,764 + +2.3 3,420,830 - -0.3 3,420,910 + -1.5 3,420,917 + +0.3 3,420,931 + -0.1 3,421,104 - -0.3 3,421,242 - Dshi_3256 0.19 -0.3 3,421,255 - Dshi_3256 0.20 -2.4 3,421,256 - Dshi_3256 0.21 -2.2 3,421,303 + Dshi_3256 0.25 +1.3 3,421,436 - Dshi_3256 0.37 +1.3 3,421,464 + Dshi_3256 0.40 -1.3 3,421,471 - Dshi_3256 0.40 -0.7 3,421,482 + Dshi_3256 0.41 +1.3 3,421,626 - Dshi_3256 0.55 +2.3 3,421,637 + Dshi_3256 0.56 +1.8
Or see this region's nucleotide sequence