Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3021 and Dshi_3022 are separated by 182 nucleotides Dshi_3022 and Dshi_3023 overlap by 4 nucleotides Dshi_3023 and Dshi_3024 are separated by 111 nucleotides
Dshi_3021: Dshi_3021 - aldehyde dehydrogenase (RefSeq), at 3,190,673 to 3,192,130
_3021
Dshi_3022: Dshi_3022 - PfkB domain protein (RefSeq), at 3,192,313 to 3,193,314
_3022
Dshi_3023: Dshi_3023 - endonuclease III (RefSeq), at 3,193,311 to 3,193,955
_3023
Dshi_3024: Dshi_3024 - transcriptional regulator, AraC family (RefSeq), at 3,194,067 to 3,194,930
_3024
Position (kb)
3192
3193
3194 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3191.398 kb on + strand, within Dshi_3021 at 3191.414 kb on + strand, within Dshi_3021 at 3191.539 kb on - strand, within Dshi_3021 at 3191.539 kb on - strand, within Dshi_3021 at 3191.596 kb on + strand, within Dshi_3021 at 3191.608 kb on + strand, within Dshi_3021 at 3191.623 kb on + strand, within Dshi_3021 at 3191.627 kb on - strand, within Dshi_3021 at 3191.639 kb on - strand, within Dshi_3021 at 3191.744 kb on + strand, within Dshi_3021 at 3191.893 kb on + strand, within Dshi_3021 at 3191.934 kb on - strand, within Dshi_3021 at 3191.989 kb on - strand at 3192.000 kb on + strand at 3192.008 kb on - strand at 3192.028 kb on + strand at 3192.061 kb on - strand at 3192.157 kb on - strand at 3192.269 kb on - strand at 3192.311 kb on + strand at 3192.392 kb on - strand at 3192.629 kb on + strand, within Dshi_3022 at 3192.642 kb on - strand, within Dshi_3022 at 3192.642 kb on - strand, within Dshi_3022 at 3192.642 kb on - strand, within Dshi_3022 at 3192.749 kb on - strand, within Dshi_3022 at 3192.788 kb on + strand, within Dshi_3022 at 3192.979 kb on - strand, within Dshi_3022 at 3193.133 kb on + strand, within Dshi_3022 at 3193.133 kb on + strand, within Dshi_3022 at 3193.231 kb on + strand at 3193.295 kb on - strand at 3193.381 kb on + strand, within Dshi_3023 at 3193.571 kb on + strand, within Dshi_3023 at 3193.639 kb on + strand, within Dshi_3023 at 3193.698 kb on + strand, within Dshi_3023 at 3193.754 kb on - strand, within Dshi_3023 at 3193.808 kb on - strand, within Dshi_3023 at 3193.844 kb on + strand, within Dshi_3023 at 3193.872 kb on - strand, within Dshi_3023 at 3193.900 kb on + strand at 3193.971 kb on - strand at 3193.971 kb on - strand at 3194.015 kb on + strand at 3194.154 kb on + strand, within Dshi_3024 at 3194.160 kb on - strand, within Dshi_3024 at 3194.253 kb on - strand, within Dshi_3024 at 3194.308 kb on + strand, within Dshi_3024
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 3,191,398 + Dshi_3021 0.50 +2.3 3,191,414 + Dshi_3021 0.51 -1.9 3,191,539 - Dshi_3021 0.59 -2.1 3,191,539 - Dshi_3021 0.59 -0.7 3,191,596 + Dshi_3021 0.63 -2.9 3,191,608 + Dshi_3021 0.64 +1.3 3,191,623 + Dshi_3021 0.65 -0.6 3,191,627 - Dshi_3021 0.65 +1.3 3,191,639 - Dshi_3021 0.66 +1.3 3,191,744 + Dshi_3021 0.73 -2.0 3,191,893 + Dshi_3021 0.84 -2.7 3,191,934 - Dshi_3021 0.86 -1.7 3,191,989 - -0.1 3,192,000 + -0.9 3,192,008 - +1.0 3,192,028 + -0.7 3,192,061 - -0.1 3,192,157 - -1.1 3,192,269 - -2.1 3,192,311 + +0.6 3,192,392 - -1.6 3,192,629 + Dshi_3022 0.32 -2.1 3,192,642 - Dshi_3022 0.33 -1.9 3,192,642 - Dshi_3022 0.33 -0.3 3,192,642 - Dshi_3022 0.33 -0.2 3,192,749 - Dshi_3022 0.44 +1.3 3,192,788 + Dshi_3022 0.47 +3.1 3,192,979 - Dshi_3022 0.66 -0.3 3,193,133 + Dshi_3022 0.82 -2.2 3,193,133 + Dshi_3022 0.82 -0.1 3,193,231 + -0.3 3,193,295 - +1.4 3,193,381 + Dshi_3023 0.11 -1.3 3,193,571 + Dshi_3023 0.40 +2.6 3,193,639 + Dshi_3023 0.51 -1.3 3,193,698 + Dshi_3023 0.60 -0.1 3,193,754 - Dshi_3023 0.69 -0.2 3,193,808 - Dshi_3023 0.77 +0.3 3,193,844 + Dshi_3023 0.83 -0.4 3,193,872 - Dshi_3023 0.87 -2.0 3,193,900 + -2.4 3,193,971 - -2.2 3,193,971 - -1.0 3,194,015 + +0.1 3,194,154 + Dshi_3024 0.10 -0.9 3,194,160 - Dshi_3024 0.11 +1.3 3,194,253 - Dshi_3024 0.22 -0.7 3,194,308 + Dshi_3024 0.28 -1.7
Or see this region's nucleotide sequence