Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_5008 and Dshi_2113 are separated by 4 nucleotides Dshi_2113 and Dshi_2114 are separated by 82 nucleotides Dshi_2114 and Dshi_2115 are separated by 13 nucleotides
Dshi_5008: Dshi_5008 - L-serine dehydratase (NCBI), at 2,240,003 to 2,241,415
_5008
Dshi_2113: Dshi_2113 - Glycine hydroxymethyltransferase (RefSeq), at 2,241,420 to 2,242,694
_2113
Dshi_2114: Dshi_2114 - Sarcosine oxidase gamma subunit (RefSeq), at 2,242,777 to 2,243,307
_2114
Dshi_2115: Dshi_2115 - sarcosine oxidase, alpha subunit family (RefSeq), at 2,243,321 to 2,246,323
_2115
Position (kb)
2241
2242
2243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2240.427 kb on - strand, within Dshi_5008 at 2240.444 kb on - strand, within Dshi_5008 at 2240.573 kb on - strand, within Dshi_5008 at 2240.606 kb on - strand, within Dshi_5008 at 2240.725 kb on + strand, within Dshi_5008 at 2240.735 kb on + strand, within Dshi_5008 at 2240.753 kb on + strand, within Dshi_5008 at 2240.768 kb on + strand, within Dshi_5008 at 2240.812 kb on + strand, within Dshi_5008 at 2240.860 kb on + strand, within Dshi_5008 at 2240.860 kb on + strand, within Dshi_5008 at 2240.868 kb on - strand, within Dshi_5008 at 2240.899 kb on + strand, within Dshi_5008 at 2240.952 kb on - strand, within Dshi_5008 at 2240.972 kb on + strand, within Dshi_5008 at 2240.972 kb on - strand, within Dshi_5008 at 2240.998 kb on + strand, within Dshi_5008 at 2241.009 kb on - strand, within Dshi_5008 at 2241.102 kb on - strand, within Dshi_5008 at 2241.163 kb on + strand, within Dshi_5008 at 2241.253 kb on + strand, within Dshi_5008 at 2241.256 kb on - strand, within Dshi_5008 at 2241.349 kb on + strand at 2241.349 kb on + strand at 2241.376 kb on - strand at 2241.534 kb on + strand at 2241.569 kb on - strand, within Dshi_2113 at 2241.609 kb on - strand, within Dshi_2113 at 2241.614 kb on + strand, within Dshi_2113 at 2241.614 kb on + strand, within Dshi_2113 at 2241.622 kb on - strand, within Dshi_2113 at 2241.622 kb on - strand, within Dshi_2113 at 2241.917 kb on + strand, within Dshi_2113 at 2241.946 kb on + strand, within Dshi_2113 at 2242.033 kb on + strand, within Dshi_2113 at 2242.073 kb on + strand, within Dshi_2113 at 2242.187 kb on + strand, within Dshi_2113 at 2242.192 kb on + strand, within Dshi_2113 at 2242.200 kb on - strand, within Dshi_2113 at 2242.254 kb on - strand, within Dshi_2113 at 2242.264 kb on - strand, within Dshi_2113 at 2242.264 kb on - strand, within Dshi_2113 at 2242.300 kb on + strand, within Dshi_2113 at 2242.342 kb on + strand, within Dshi_2113 at 2242.342 kb on - strand, within Dshi_2113 at 2242.475 kb on - strand, within Dshi_2113 at 2242.475 kb on - strand, within Dshi_2113 at 2242.495 kb on - strand, within Dshi_2113 at 2242.725 kb on - strand at 2242.776 kb on + strand at 2242.820 kb on - strand at 2243.028 kb on + strand, within Dshi_2114 at 2243.028 kb on + strand, within Dshi_2114 at 2243.083 kb on + strand, within Dshi_2114 at 2243.137 kb on - strand, within Dshi_2114 at 2243.139 kb on - strand, within Dshi_2114 at 2243.139 kb on - strand, within Dshi_2114 at 2243.185 kb on + strand, within Dshi_2114 at 2243.193 kb on - strand, within Dshi_2114 at 2243.214 kb on - strand, within Dshi_2114 at 2243.309 kb on + strand at 2243.330 kb on - strand at 2243.431 kb on + strand at 2243.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 2,240,427 - Dshi_5008 0.30 -2.0 2,240,444 - Dshi_5008 0.31 +0.0 2,240,573 - Dshi_5008 0.40 -1.0 2,240,606 - Dshi_5008 0.43 -2.0 2,240,725 + Dshi_5008 0.51 +0.1 2,240,735 + Dshi_5008 0.52 -0.0 2,240,753 + Dshi_5008 0.53 +1.0 2,240,768 + Dshi_5008 0.54 -0.7 2,240,812 + Dshi_5008 0.57 -1.4 2,240,860 + Dshi_5008 0.61 -2.7 2,240,860 + Dshi_5008 0.61 -0.0 2,240,868 - Dshi_5008 0.61 +0.3 2,240,899 + Dshi_5008 0.63 -2.7 2,240,952 - Dshi_5008 0.67 +0.1 2,240,972 + Dshi_5008 0.69 -0.0 2,240,972 - Dshi_5008 0.69 -3.7 2,240,998 + Dshi_5008 0.70 +1.0 2,241,009 - Dshi_5008 0.71 -2.6 2,241,102 - Dshi_5008 0.78 -3.2 2,241,163 + Dshi_5008 0.82 -1.5 2,241,253 + Dshi_5008 0.88 +2.4 2,241,256 - Dshi_5008 0.89 +1.0 2,241,349 + -0.3 2,241,349 + +1.0 2,241,376 - -0.6 2,241,534 + +0.8 2,241,569 - Dshi_2113 0.12 -0.1 2,241,609 - Dshi_2113 0.15 -1.6 2,241,614 + Dshi_2113 0.15 -1.1 2,241,614 + Dshi_2113 0.15 +1.4 2,241,622 - Dshi_2113 0.16 -0.9 2,241,622 - Dshi_2113 0.16 -0.1 2,241,917 + Dshi_2113 0.39 -0.5 2,241,946 + Dshi_2113 0.41 -1.5 2,242,033 + Dshi_2113 0.48 -0.0 2,242,073 + Dshi_2113 0.51 +0.7 2,242,187 + Dshi_2113 0.60 -3.2 2,242,192 + Dshi_2113 0.61 +1.0 2,242,200 - Dshi_2113 0.61 -2.7 2,242,254 - Dshi_2113 0.65 +0.3 2,242,264 - Dshi_2113 0.66 -0.9 2,242,264 - Dshi_2113 0.66 +1.0 2,242,300 + Dshi_2113 0.69 -0.8 2,242,342 + Dshi_2113 0.72 -1.9 2,242,342 - Dshi_2113 0.72 -0.5 2,242,475 - Dshi_2113 0.83 +3.5 2,242,475 - Dshi_2113 0.83 +1.0 2,242,495 - Dshi_2113 0.84 +1.0 2,242,725 - -2.6 2,242,776 + -1.6 2,242,820 - +1.0 2,243,028 + Dshi_2114 0.47 -2.1 2,243,028 + Dshi_2114 0.47 -2.0 2,243,083 + Dshi_2114 0.58 -2.0 2,243,137 - Dshi_2114 0.68 +0.6 2,243,139 - Dshi_2114 0.68 -1.4 2,243,139 - Dshi_2114 0.68 +3.5 2,243,185 + Dshi_2114 0.77 +0.4 2,243,193 - Dshi_2114 0.78 -0.0 2,243,214 - Dshi_2114 0.82 -1.5 2,243,309 + -0.9 2,243,330 - -2.7 2,243,431 + -1.0 2,243,487 - -0.9
Or see this region's nucleotide sequence