Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2109 and Dshi_2110 are separated by 141 nucleotides Dshi_2110 and Dshi_2111 are separated by 29 nucleotides Dshi_2111 and Dshi_5008 overlap by 35 nucleotides
Dshi_2109: Dshi_2109 - alanine racemase domain protein (RefSeq), at 2,236,892 to 2,238,043
_2109
Dshi_2110: Dshi_2110 - Indigoidine synthase A family protein (RefSeq), at 2,238,185 to 2,239,102
_2110
Dshi_2111: Dshi_2111 - formyltetrahydrofolate deformylase (RefSeq), at 2,239,132 to 2,240,037
_2111
Dshi_5008: Dshi_5008 - L-serine dehydratase (NCBI), at 2,240,003 to 2,241,415
_5008
Position (kb)
2238
2239
2240 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2237.328 kb on - strand, within Dshi_2109 at 2237.368 kb on - strand, within Dshi_2109 at 2237.394 kb on - strand, within Dshi_2109 at 2237.399 kb on - strand, within Dshi_2109 at 2237.416 kb on - strand, within Dshi_2109 at 2237.477 kb on + strand, within Dshi_2109 at 2237.522 kb on + strand, within Dshi_2109 at 2237.579 kb on - strand, within Dshi_2109 at 2237.581 kb on + strand, within Dshi_2109 at 2237.682 kb on + strand, within Dshi_2109 at 2237.741 kb on + strand, within Dshi_2109 at 2237.769 kb on - strand, within Dshi_2109 at 2237.774 kb on - strand, within Dshi_2109 at 2237.796 kb on - strand, within Dshi_2109 at 2237.834 kb on + strand, within Dshi_2109 at 2237.951 kb on - strand at 2238.008 kb on - strand at 2238.019 kb on + strand at 2238.043 kb on + strand at 2238.043 kb on + strand at 2238.146 kb on - strand at 2238.168 kb on - strand at 2238.168 kb on - strand at 2238.209 kb on - strand at 2238.290 kb on - strand, within Dshi_2110 at 2238.316 kb on + strand, within Dshi_2110 at 2238.324 kb on - strand, within Dshi_2110 at 2238.441 kb on - strand, within Dshi_2110 at 2238.441 kb on - strand, within Dshi_2110 at 2238.472 kb on - strand, within Dshi_2110 at 2238.551 kb on - strand, within Dshi_2110 at 2238.551 kb on - strand, within Dshi_2110 at 2238.639 kb on + strand, within Dshi_2110 at 2238.643 kb on + strand, within Dshi_2110 at 2238.644 kb on - strand, within Dshi_2110 at 2238.693 kb on - strand, within Dshi_2110 at 2238.718 kb on - strand, within Dshi_2110 at 2238.899 kb on + strand, within Dshi_2110 at 2239.062 kb on - strand at 2239.088 kb on + strand at 2239.096 kb on - strand at 2239.201 kb on + strand at 2239.201 kb on + strand at 2239.209 kb on - strand at 2239.209 kb on - strand at 2239.253 kb on + strand, within Dshi_2111 at 2239.352 kb on - strand, within Dshi_2111 at 2239.365 kb on - strand, within Dshi_2111 at 2239.394 kb on - strand, within Dshi_2111 at 2239.462 kb on + strand, within Dshi_2111 at 2239.528 kb on + strand, within Dshi_2111 at 2239.535 kb on + strand, within Dshi_2111 at 2239.536 kb on - strand, within Dshi_2111 at 2239.563 kb on - strand, within Dshi_2111 at 2239.658 kb on + strand, within Dshi_2111 at 2239.738 kb on + strand, within Dshi_2111 at 2239.738 kb on + strand, within Dshi_2111 at 2239.746 kb on - strand, within Dshi_2111 at 2239.746 kb on - strand, within Dshi_2111 at 2239.864 kb on + strand, within Dshi_2111 at 2239.888 kb on - strand, within Dshi_2111 at 2239.936 kb on + strand, within Dshi_2111 at 2239.957 kb on - strand at 2240.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 2,237,328 - Dshi_2109 0.38 -1.3 2,237,368 - Dshi_2109 0.41 +1.0 2,237,394 - Dshi_2109 0.44 +0.4 2,237,399 - Dshi_2109 0.44 -0.6 2,237,416 - Dshi_2109 0.45 -2.7 2,237,477 + Dshi_2109 0.51 +1.6 2,237,522 + Dshi_2109 0.55 -2.5 2,237,579 - Dshi_2109 0.60 +1.6 2,237,581 + Dshi_2109 0.60 +0.2 2,237,682 + Dshi_2109 0.69 -3.0 2,237,741 + Dshi_2109 0.74 +3.0 2,237,769 - Dshi_2109 0.76 +3.0 2,237,774 - Dshi_2109 0.77 +2.2 2,237,796 - Dshi_2109 0.78 +1.0 2,237,834 + Dshi_2109 0.82 +1.0 2,237,951 - +1.0 2,238,008 - -0.0 2,238,019 + +2.0 2,238,043 + -0.7 2,238,043 + -1.4 2,238,146 - +0.7 2,238,168 - -0.0 2,238,168 - +1.0 2,238,209 - -0.6 2,238,290 - Dshi_2110 0.11 -0.6 2,238,316 + Dshi_2110 0.14 +0.7 2,238,324 - Dshi_2110 0.15 -1.8 2,238,441 - Dshi_2110 0.28 -1.4 2,238,441 - Dshi_2110 0.28 -2.2 2,238,472 - Dshi_2110 0.31 -1.9 2,238,551 - Dshi_2110 0.40 -1.9 2,238,551 - Dshi_2110 0.40 -0.6 2,238,639 + Dshi_2110 0.49 +2.0 2,238,643 + Dshi_2110 0.50 +1.6 2,238,644 - Dshi_2110 0.50 +0.7 2,238,693 - Dshi_2110 0.55 -0.2 2,238,718 - Dshi_2110 0.58 -1.1 2,238,899 + Dshi_2110 0.78 -1.0 2,239,062 - +0.2 2,239,088 + -1.1 2,239,096 - +1.0 2,239,201 + +2.0 2,239,201 + -2.0 2,239,209 - -0.0 2,239,209 - -3.6 2,239,253 + Dshi_2111 0.13 +1.0 2,239,352 - Dshi_2111 0.24 +0.4 2,239,365 - Dshi_2111 0.26 -2.2 2,239,394 - Dshi_2111 0.29 -0.6 2,239,462 + Dshi_2111 0.36 -0.5 2,239,528 + Dshi_2111 0.44 -0.4 2,239,535 + Dshi_2111 0.44 +0.1 2,239,536 - Dshi_2111 0.45 -0.6 2,239,563 - Dshi_2111 0.48 +1.0 2,239,658 + Dshi_2111 0.58 -2.1 2,239,738 + Dshi_2111 0.67 -2.5 2,239,738 + Dshi_2111 0.67 +1.2 2,239,746 - Dshi_2111 0.68 +2.6 2,239,746 - Dshi_2111 0.68 -1.8 2,239,864 + Dshi_2111 0.81 +1.0 2,239,888 - Dshi_2111 0.83 -0.3 2,239,936 + Dshi_2111 0.89 -1.4 2,239,957 - -0.2 2,240,065 + +1.0
Or see this region's nucleotide sequence