Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1868 and Dshi_1869 are separated by 20 nucleotides Dshi_1869 and Dshi_1870 overlap by 1 nucleotides
Dshi_1868: Dshi_1868 - ferredoxin-dependent glutamate synthase (RefSeq), at 1,947,542 to 1,948,876
_1868
Dshi_1869: Dshi_1869 - glutamate synthase alpha subunit domain protein (RefSeq), at 1,948,897 to 1,949,574
_1869
Dshi_1870: Dshi_1870 - glutamine amidotransferase class-II (RefSeq), at 1,949,574 to 1,950,500
_1870
Position (kb)
1948
1949
1950 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1947.944 kb on + strand, within Dshi_1868 at 1947.957 kb on - strand, within Dshi_1868 at 1948.117 kb on - strand, within Dshi_1868 at 1948.137 kb on + strand, within Dshi_1868 at 1948.289 kb on - strand, within Dshi_1868 at 1948.430 kb on + strand, within Dshi_1868 at 1948.518 kb on - strand, within Dshi_1868 at 1948.553 kb on - strand, within Dshi_1868 at 1948.719 kb on + strand, within Dshi_1868 at 1948.784 kb on - strand at 1949.064 kb on - strand, within Dshi_1869 at 1949.139 kb on - strand, within Dshi_1869 at 1949.139 kb on - strand, within Dshi_1869 at 1949.141 kb on + strand at 1949.364 kb on + strand, within Dshi_1869 at 1949.388 kb on - strand, within Dshi_1869 at 1949.388 kb on - strand, within Dshi_1869 at 1949.481 kb on - strand, within Dshi_1869 at 1949.496 kb on + strand, within Dshi_1869 at 1949.556 kb on - strand at 1949.595 kb on + strand at 1949.603 kb on - strand at 1949.627 kb on - strand at 1949.672 kb on + strand, within Dshi_1870 at 1949.789 kb on - strand, within Dshi_1870 at 1949.830 kb on - strand, within Dshi_1870 at 1949.837 kb on - strand, within Dshi_1870 at 1949.958 kb on - strand, within Dshi_1870 at 1950.046 kb on + strand, within Dshi_1870 at 1950.208 kb on + strand, within Dshi_1870 at 1950.231 kb on + strand, within Dshi_1870 at 1950.239 kb on - strand, within Dshi_1870 at 1950.404 kb on - strand, within Dshi_1870
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 1,947,944 + Dshi_1868 0.30 -2.3 1,947,957 - Dshi_1868 0.31 -0.6 1,948,117 - Dshi_1868 0.43 +0.9 1,948,137 + Dshi_1868 0.45 -0.6 1,948,289 - Dshi_1868 0.56 -1.2 1,948,430 + Dshi_1868 0.67 +0.9 1,948,518 - Dshi_1868 0.73 +1.1 1,948,553 - Dshi_1868 0.76 +4.3 1,948,719 + Dshi_1868 0.88 -2.8 1,948,784 - +2.8 1,949,064 - Dshi_1869 0.25 +1.0 1,949,139 - Dshi_1869 0.36 +1.0 1,949,139 - Dshi_1869 0.36 +2.0 1,949,141 + +1.0 1,949,364 + Dshi_1869 0.69 +3.0 1,949,388 - Dshi_1869 0.72 +1.5 1,949,388 - Dshi_1869 0.72 -0.7 1,949,481 - Dshi_1869 0.86 +0.7 1,949,496 + Dshi_1869 0.88 -2.5 1,949,556 - -0.6 1,949,595 + -3.4 1,949,603 - -1.2 1,949,627 - -0.5 1,949,672 + Dshi_1870 0.11 -1.0 1,949,789 - Dshi_1870 0.23 +1.0 1,949,830 - Dshi_1870 0.28 -1.2 1,949,837 - Dshi_1870 0.28 +1.0 1,949,958 - Dshi_1870 0.41 -2.0 1,950,046 + Dshi_1870 0.51 +2.8 1,950,208 + Dshi_1870 0.68 -1.2 1,950,231 + Dshi_1870 0.71 -0.0 1,950,239 - Dshi_1870 0.72 -1.3 1,950,404 - Dshi_1870 0.90 +2.3
Or see this region's nucleotide sequence