Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1577 and Dshi_1578 overlap by 4 nucleotides Dshi_1578 and Dshi_1579 overlap by 4 nucleotides Dshi_1579 and Dshi_1580 overlap by 22 nucleotides Dshi_1580 and Dshi_1581 are separated by 23 nucleotides
Dshi_1577: Dshi_1577 - 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (RefSeq), at 1,644,540 to 1,645,742
_1577
Dshi_1578: Dshi_1578 - phosphatidylglycerophosphatase A (RefSeq), at 1,645,739 to 1,646,227
_1578
Dshi_1579: Dshi_1579 - CinA domain protein (RefSeq), at 1,646,224 to 1,646,706
_1579
Dshi_1580: Dshi_1580 - cyclase/dehydrase (RefSeq), at 1,646,685 to 1,647,200
_1580
Dshi_1581: Dshi_1581 - hypoxanthine phosphoribosyltransferase (RefSeq), at 1,647,224 to 1,647,760
_1581
Position (kb)
1646
1647 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1645.736 kb on + strand at 1645.738 kb on + strand at 1646.264 kb on + strand at 1646.416 kb on - strand, within Dshi_1579 at 1646.422 kb on - strand, within Dshi_1579 at 1646.683 kb on + strand at 1646.693 kb on + strand at 1646.900 kb on - strand, within Dshi_1580 at 1647.063 kb on - strand, within Dshi_1580 at 1647.076 kb on + strand, within Dshi_1580 at 1647.084 kb on - strand, within Dshi_1580 at 1647.093 kb on + strand, within Dshi_1580 at 1647.094 kb on - strand, within Dshi_1580 at 1647.103 kb on + strand, within Dshi_1580 at 1647.114 kb on - strand, within Dshi_1580 at 1647.276 kb on - strand at 1647.341 kb on - strand, within Dshi_1581 at 1647.384 kb on + strand, within Dshi_1581 at 1647.427 kb on + strand, within Dshi_1581 at 1647.548 kb on + strand, within Dshi_1581 at 1647.561 kb on - strand, within Dshi_1581 at 1647.579 kb on + strand, within Dshi_1581 at 1647.621 kb on + strand, within Dshi_1581
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 1,645,736 + -0.8 1,645,738 + -0.4 1,646,264 + +3.8 1,646,416 - Dshi_1579 0.40 +0.8 1,646,422 - Dshi_1579 0.41 -1.0 1,646,683 + +1.4 1,646,693 + -0.7 1,646,900 - Dshi_1580 0.42 +0.5 1,647,063 - Dshi_1580 0.73 +1.8 1,647,076 + Dshi_1580 0.76 +1.7 1,647,084 - Dshi_1580 0.77 -0.9 1,647,093 + Dshi_1580 0.79 +0.4 1,647,094 - Dshi_1580 0.79 -1.7 1,647,103 + Dshi_1580 0.81 -2.0 1,647,114 - Dshi_1580 0.83 -1.2 1,647,276 - -3.0 1,647,341 - Dshi_1581 0.22 +0.4 1,647,384 + Dshi_1581 0.30 -1.2 1,647,427 + Dshi_1581 0.38 -0.2 1,647,548 + Dshi_1581 0.60 +0.1 1,647,561 - Dshi_1581 0.63 +3.3 1,647,579 + Dshi_1581 0.66 +1.5 1,647,621 + Dshi_1581 0.74 -0.9
Or see this region's nucleotide sequence