Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1507 and Dshi_1508 are separated by 99 nucleotides Dshi_1508 and Dshi_1509 are separated by 39 nucleotides Dshi_1509 and Dshi_1510 overlap by 4 nucleotides
Dshi_1507: Dshi_1507 - protein of unknown function DUF1153 (RefSeq), at 1,567,599 to 1,567,877
_1507
Dshi_1508: Dshi_1508 - two component transcriptional regulator, winged helix family (RefSeq), at 1,567,977 to 1,568,690
_1508
Dshi_1509: Dshi_1509 - DNA ligase, NAD-dependent (RefSeq), at 1,568,730 to 1,570,955
_1509
Dshi_1510: Dshi_1510 - DEAD/DEAH box helicase domain protein (RefSeq), at 1,570,952 to 1,573,042
_1510
Position (kb)
1568
1569
1570
1571 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1567.737 kb on - strand, within Dshi_1507 at 1567.740 kb on + strand, within Dshi_1507 at 1567.939 kb on + strand at 1567.947 kb on - strand at 1567.947 kb on - strand at 1567.969 kb on - strand at 1568.027 kb on - strand at 1568.031 kb on + strand at 1568.045 kb on - strand at 1568.062 kb on + strand, within Dshi_1508 at 1568.068 kb on - strand, within Dshi_1508 at 1568.068 kb on - strand, within Dshi_1508 at 1568.123 kb on + strand, within Dshi_1508 at 1568.136 kb on - strand, within Dshi_1508 at 1568.149 kb on - strand, within Dshi_1508 at 1568.168 kb on - strand, within Dshi_1508 at 1568.188 kb on - strand, within Dshi_1508 at 1568.275 kb on - strand, within Dshi_1508 at 1568.338 kb on - strand, within Dshi_1508 at 1568.415 kb on - strand, within Dshi_1508 at 1568.486 kb on - strand, within Dshi_1508 at 1568.698 kb on + strand at 1568.699 kb on - strand at 1568.701 kb on + strand at 1568.819 kb on + strand at 1568.829 kb on + strand at 1571.001 kb on - strand at 1571.079 kb on - strand at 1571.197 kb on - strand, within Dshi_1510 at 1571.373 kb on - strand, within Dshi_1510 at 1571.600 kb on - strand, within Dshi_1510 at 1571.620 kb on + strand, within Dshi_1510 at 1571.797 kb on + strand, within Dshi_1510
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 1,567,737 - Dshi_1507 0.49 -0.2 1,567,740 + Dshi_1507 0.51 -1.7 1,567,939 + -3.7 1,567,947 - +3.2 1,567,947 - +0.5 1,567,969 - +0.5 1,568,027 - +0.2 1,568,031 + +0.5 1,568,045 - +0.9 1,568,062 + Dshi_1508 0.12 -0.2 1,568,068 - Dshi_1508 0.13 +0.7 1,568,068 - Dshi_1508 0.13 -1.6 1,568,123 + Dshi_1508 0.20 +1.2 1,568,136 - Dshi_1508 0.22 +1.2 1,568,149 - Dshi_1508 0.24 +1.3 1,568,168 - Dshi_1508 0.27 +1.5 1,568,188 - Dshi_1508 0.30 +1.0 1,568,275 - Dshi_1508 0.42 +1.2 1,568,338 - Dshi_1508 0.51 +1.3 1,568,415 - Dshi_1508 0.61 +0.7 1,568,486 - Dshi_1508 0.71 -3.1 1,568,698 + -2.0 1,568,699 - -1.0 1,568,701 + +1.8 1,568,819 + -1.1 1,568,829 + +1.1 1,571,001 - -1.0 1,571,079 - -0.8 1,571,197 - Dshi_1510 0.12 +0.2 1,571,373 - Dshi_1510 0.20 +0.8 1,571,600 - Dshi_1510 0.31 -1.5 1,571,620 + Dshi_1510 0.32 -1.6 1,571,797 + Dshi_1510 0.40 +0.8
Or see this region's nucleotide sequence