Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1264 and Dshi_1265 are separated by 19 nucleotides Dshi_1265 and Dshi_1266 overlap by 4 nucleotides Dshi_1266 and Dshi_1267 overlap by 4 nucleotides Dshi_1267 and Dshi_1268 are separated by 3 nucleotides
Dshi_1264: Dshi_1264 - Ion transport 2 domain protein (RefSeq), at 1,313,689 to 1,314,135
_1264
Dshi_1265: Dshi_1265 - Inositol 2-dehydrogenase (RefSeq), at 1,314,155 to 1,315,138
_1265
Dshi_1266: Dshi_1266 - ketose-bisphosphate aldolase (RefSeq), at 1,315,135 to 1,315,977
_1266
Dshi_1267: Dshi_1267 - hypothetical protein (RefSeq), at 1,315,974 to 1,316,834
_1267
Dshi_1268: Dshi_1268 - PfkB domain protein (RefSeq), at 1,316,838 to 1,317,839
_1268
Position (kb)
1315
1316 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1314.143 kb on + strand at 1314.148 kb on + strand at 1314.189 kb on + strand at 1314.217 kb on - strand at 1314.225 kb on - strand at 1314.285 kb on + strand, within Dshi_1265 at 1314.303 kb on + strand, within Dshi_1265 at 1314.483 kb on - strand, within Dshi_1265 at 1314.514 kb on - strand, within Dshi_1265 at 1314.769 kb on + strand, within Dshi_1265 at 1314.838 kb on - strand, within Dshi_1265 at 1314.935 kb on - strand, within Dshi_1265 at 1315.014 kb on + strand, within Dshi_1265 at 1315.119 kb on - strand at 1315.242 kb on + strand, within Dshi_1266 at 1315.250 kb on - strand, within Dshi_1266 at 1315.274 kb on + strand, within Dshi_1266 at 1315.293 kb on + strand, within Dshi_1266 at 1315.421 kb on + strand, within Dshi_1266 at 1315.491 kb on - strand, within Dshi_1266 at 1315.625 kb on - strand, within Dshi_1266 at 1315.703 kb on + strand, within Dshi_1266 at 1315.915 kb on + strand at 1315.920 kb on + strand at 1315.928 kb on - strand at 1316.027 kb on - strand at 1316.074 kb on - strand, within Dshi_1267 at 1316.131 kb on - strand, within Dshi_1267 at 1316.216 kb on + strand, within Dshi_1267 at 1316.266 kb on - strand, within Dshi_1267 at 1316.277 kb on - strand, within Dshi_1267 at 1316.316 kb on - strand, within Dshi_1267 at 1316.325 kb on + strand, within Dshi_1267 at 1316.352 kb on - strand, within Dshi_1267 at 1316.372 kb on + strand, within Dshi_1267 at 1316.434 kb on + strand, within Dshi_1267 at 1316.473 kb on - strand, within Dshi_1267 at 1316.560 kb on - strand, within Dshi_1267 at 1316.590 kb on - strand, within Dshi_1267 at 1316.675 kb on + strand, within Dshi_1267 at 1316.706 kb on + strand, within Dshi_1267 at 1316.714 kb on - strand, within Dshi_1267 at 1316.822 kb on - strand at 1316.836 kb on + strand at 1316.934 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 1,314,143 + +0.4 1,314,148 + +2.1 1,314,189 + -0.7 1,314,217 - -0.5 1,314,225 - -0.7 1,314,285 + Dshi_1265 0.13 -1.3 1,314,303 + Dshi_1265 0.15 +0.7 1,314,483 - Dshi_1265 0.33 +0.1 1,314,514 - Dshi_1265 0.36 +0.1 1,314,769 + Dshi_1265 0.62 -2.8 1,314,838 - Dshi_1265 0.69 -1.6 1,314,935 - Dshi_1265 0.79 -1.9 1,315,014 + Dshi_1265 0.87 -0.5 1,315,119 - -2.5 1,315,242 + Dshi_1266 0.13 +0.3 1,315,250 - Dshi_1266 0.14 -0.2 1,315,274 + Dshi_1266 0.16 +0.2 1,315,293 + Dshi_1266 0.19 -1.9 1,315,421 + Dshi_1266 0.34 -0.5 1,315,491 - Dshi_1266 0.42 -3.3 1,315,625 - Dshi_1266 0.58 -2.5 1,315,703 + Dshi_1266 0.67 +1.1 1,315,915 + -1.7 1,315,920 + +0.4 1,315,928 - +1.1 1,316,027 - +1.8 1,316,074 - Dshi_1267 0.12 -0.5 1,316,131 - Dshi_1267 0.18 -0.5 1,316,216 + Dshi_1267 0.28 +2.1 1,316,266 - Dshi_1267 0.34 +0.5 1,316,277 - Dshi_1267 0.35 +0.1 1,316,316 - Dshi_1267 0.40 -0.5 1,316,325 + Dshi_1267 0.41 +0.4 1,316,352 - Dshi_1267 0.44 +0.8 1,316,372 + Dshi_1267 0.46 +0.1 1,316,434 + Dshi_1267 0.53 +0.3 1,316,473 - Dshi_1267 0.58 -1.3 1,316,560 - Dshi_1267 0.68 -2.8 1,316,590 - Dshi_1267 0.72 -2.1 1,316,675 + Dshi_1267 0.81 -0.4 1,316,706 + Dshi_1267 0.85 -1.3 1,316,714 - Dshi_1267 0.86 -0.7 1,316,822 - -1.3 1,316,836 + -1.1 1,316,934 - -0.7
Or see this region's nucleotide sequence