Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1212 and Dshi_1213 are separated by 41 nucleotides Dshi_1213 and Dshi_1214 overlap by 4 nucleotides Dshi_1214 and Dshi_1215 overlap by 1 nucleotides Dshi_1215 and Dshi_1216 are separated by 98 nucleotides
Dshi_1212: Dshi_1212 - carbon-monoxide dehydrogenase, large subunit (RefSeq), at 1,250,367 to 1,252,787
_1212
Dshi_1213: Dshi_1213 - ATPase associated with various cellular activities AAA_5 (RefSeq), at 1,252,829 to 1,253,737
_1213
Dshi_1214: Dshi_1214 - VWA containing CoxE family protein (RefSeq), at 1,253,734 to 1,254,912
_1214
Dshi_1215: Dshi_1215 - protein of unknown function DUF182 (RefSeq), at 1,254,912 to 1,255,724
_1215
Dshi_1216: Dshi_1216 - 2-dehydropantoate 2-reductase (RefSeq), at 1,255,823 to 1,256,800
_1216
Position (kb)
1253
1254
1255 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1252.808 kb on + strand at 1252.815 kb on - strand at 1252.816 kb on - strand at 1252.863 kb on - strand at 1252.924 kb on + strand, within Dshi_1213 at 1253.021 kb on + strand, within Dshi_1213 at 1253.063 kb on - strand, within Dshi_1213 at 1253.322 kb on + strand, within Dshi_1213 at 1253.340 kb on - strand, within Dshi_1213 at 1253.621 kb on - strand, within Dshi_1213 at 1253.706 kb on - strand at 1253.818 kb on + strand at 1253.819 kb on - strand at 1253.883 kb on - strand, within Dshi_1214 at 1253.913 kb on - strand, within Dshi_1214 at 1253.934 kb on + strand, within Dshi_1214 at 1254.005 kb on - strand, within Dshi_1214 at 1254.005 kb on - strand, within Dshi_1214 at 1254.005 kb on - strand, within Dshi_1214 at 1254.039 kb on - strand, within Dshi_1214 at 1254.476 kb on - strand, within Dshi_1214 at 1254.527 kb on + strand, within Dshi_1214 at 1254.527 kb on + strand, within Dshi_1214 at 1254.528 kb on - strand, within Dshi_1214 at 1254.535 kb on - strand, within Dshi_1214 at 1254.535 kb on - strand, within Dshi_1214 at 1254.619 kb on + strand, within Dshi_1214 at 1254.867 kb on - strand at 1254.882 kb on - strand at 1255.042 kb on + strand, within Dshi_1215 at 1255.094 kb on - strand, within Dshi_1215 at 1255.094 kb on - strand, within Dshi_1215 at 1255.158 kb on + strand, within Dshi_1215 at 1255.158 kb on + strand, within Dshi_1215 at 1255.200 kb on + strand, within Dshi_1215 at 1255.443 kb on + strand, within Dshi_1215 at 1255.610 kb on + strand, within Dshi_1215
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 1,252,808 + -2.7 1,252,815 - -1.9 1,252,816 - -2.7 1,252,863 - +1.4 1,252,924 + Dshi_1213 0.10 +1.1 1,253,021 + Dshi_1213 0.21 -0.1 1,253,063 - Dshi_1213 0.26 +0.7 1,253,322 + Dshi_1213 0.54 -0.4 1,253,340 - Dshi_1213 0.56 +0.5 1,253,621 - Dshi_1213 0.87 +1.1 1,253,706 - -2.5 1,253,818 + -1.3 1,253,819 - +1.3 1,253,883 - Dshi_1214 0.13 -1.7 1,253,913 - Dshi_1214 0.15 +2.6 1,253,934 + Dshi_1214 0.17 -0.2 1,254,005 - Dshi_1214 0.23 +0.1 1,254,005 - Dshi_1214 0.23 +0.4 1,254,005 - Dshi_1214 0.23 +2.6 1,254,039 - Dshi_1214 0.26 +3.2 1,254,476 - Dshi_1214 0.63 -1.9 1,254,527 + Dshi_1214 0.67 +0.1 1,254,527 + Dshi_1214 0.67 -2.5 1,254,528 - Dshi_1214 0.67 +3.4 1,254,535 - Dshi_1214 0.68 +3.6 1,254,535 - Dshi_1214 0.68 -2.6 1,254,619 + Dshi_1214 0.75 +0.1 1,254,867 - -2.8 1,254,882 - +1.1 1,255,042 + Dshi_1215 0.16 +0.3 1,255,094 - Dshi_1215 0.22 +1.1 1,255,094 - Dshi_1215 0.22 -1.5 1,255,158 + Dshi_1215 0.30 -1.3 1,255,158 + Dshi_1215 0.30 -0.9 1,255,200 + Dshi_1215 0.35 +1.5 1,255,443 + Dshi_1215 0.65 +1.1 1,255,610 + Dshi_1215 0.86 +1.1
Or see this region's nucleotide sequence