Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1105 and Dshi_1106 are separated by 63 nucleotides Dshi_1106 and Dshi_1107 overlap by 4 nucleotides Dshi_1107 and Dshi_1108 are separated by 95 nucleotides Dshi_1108 and Dshi_1109 are separated by 87 nucleotides
Dshi_1105: Dshi_1105 - Holliday junction DNA helicase RuvB (RefSeq), at 1,131,167 to 1,132,240
_1105
Dshi_1106: Dshi_1106 - hypothetical protein (RefSeq), at 1,132,304 to 1,132,930
_1106
Dshi_1107: Dshi_1107 - Pol-Pal system-associated acyl-CoA thioesterase (RefSeq), at 1,132,927 to 1,133,337
_1107
Dshi_1108: Dshi_1108 - Tol-Pal system TolQ (RefSeq), at 1,133,433 to 1,134,107
_1108
Dshi_1109: Dshi_1109 - Biopolymer transport TolR (RefSeq), at 1,134,195 to 1,134,599
_1109
Position (kb)
1132
1133
1134 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1131.937 kb on - strand, within Dshi_1105 at 1132.198 kb on + strand at 1132.289 kb on + strand at 1132.290 kb on + strand at 1132.297 kb on - strand at 1132.298 kb on - strand at 1132.298 kb on - strand at 1132.298 kb on - strand at 1132.353 kb on + strand at 1132.374 kb on + strand at 1132.431 kb on - strand, within Dshi_1106 at 1132.594 kb on + strand, within Dshi_1106 at 1132.770 kb on + strand, within Dshi_1106 at 1132.798 kb on + strand, within Dshi_1106 at 1133.018 kb on + strand, within Dshi_1107 at 1133.038 kb on - strand, within Dshi_1107 at 1133.091 kb on - strand, within Dshi_1107 at 1133.148 kb on + strand, within Dshi_1107 at 1133.219 kb on + strand, within Dshi_1107 at 1133.278 kb on - strand, within Dshi_1107 at 1133.316 kb on - strand at 1133.422 kb on - strand at 1133.432 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 1,131,937 - Dshi_1105 0.72 +1.0 1,132,198 + -0.5 1,132,289 + -1.8 1,132,290 + +1.4 1,132,297 - -1.0 1,132,298 - +1.3 1,132,298 - -3.0 1,132,298 - +3.3 1,132,353 + -0.9 1,132,374 + +1.0 1,132,431 - Dshi_1106 0.20 +1.1 1,132,594 + Dshi_1106 0.46 -1.2 1,132,770 + Dshi_1106 0.74 +3.0 1,132,798 + Dshi_1106 0.79 +0.2 1,133,018 + Dshi_1107 0.22 +0.4 1,133,038 - Dshi_1107 0.27 -0.3 1,133,091 - Dshi_1107 0.40 -0.0 1,133,148 + Dshi_1107 0.54 -0.1 1,133,219 + Dshi_1107 0.71 +1.0 1,133,278 - Dshi_1107 0.85 -2.2 1,133,316 - -2.4 1,133,422 - -1.8 1,133,432 - -0.6
Or see this region's nucleotide sequence