Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0911 and Dshi_0912 overlap by 4 nucleotides Dshi_0912 and Dshi_0913 are separated by 3 nucleotides Dshi_0913 and Dshi_0914 overlap by 17 nucleotides Dshi_0914 and Dshi_0915 overlap by 4 nucleotides
Dshi_0911: Dshi_0911 - Thiamine-phosphate diphosphorylase (RefSeq), at 934,241 to 934,840
_0911
Dshi_0912: Dshi_0912 - thiazole biosynthesis family protein (RefSeq), at 934,837 to 935,631
_0912
Dshi_0913: Dshi_0913 - thiamine biosynthesis protein ThiS (RefSeq), at 935,635 to 935,829
_0913
Dshi_0914: Dshi_0914 - glycine oxidase ThiO (RefSeq), at 935,813 to 936,790
_0914
Dshi_0915: Dshi_0915 - phosphomethylpyrimidine kinase (RefSeq), at 936,787 to 937,584
_0915
Position (kb) 935
936 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 934.676 kb on + strand, within Dshi_0911 at 934.761 kb on + strand, within Dshi_0911 at 935.087 kb on - strand, within Dshi_0912 at 935.127 kb on + strand, within Dshi_0912 at 935.169 kb on - strand, within Dshi_0912 at 935.274 kb on + strand, within Dshi_0912 at 935.376 kb on - strand, within Dshi_0912 at 935.457 kb on - strand, within Dshi_0912 at 935.621 kb on + strand at 935.621 kb on + strand at 935.694 kb on - strand, within Dshi_0913 at 935.884 kb on + strand at 936.063 kb on - strand, within Dshi_0914 at 936.195 kb on - strand, within Dshi_0914 at 936.394 kb on - strand, within Dshi_0914 at 936.573 kb on + strand, within Dshi_0914 at 936.607 kb on - strand, within Dshi_0914 at 936.668 kb on + strand, within Dshi_0914
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 934,676 + Dshi_0911 0.72 -0.5 934,761 + Dshi_0911 0.87 +0.5 935,087 - Dshi_0912 0.31 +0.2 935,127 + Dshi_0912 0.36 +2.2 935,169 - Dshi_0912 0.42 +0.7 935,274 + Dshi_0912 0.55 -1.3 935,376 - Dshi_0912 0.68 -1.1 935,457 - Dshi_0912 0.78 +1.3 935,621 + -1.3 935,621 + +0.3 935,694 - Dshi_0913 0.30 +3.3 935,884 + +1.0 936,063 - Dshi_0914 0.26 +0.0 936,195 - Dshi_0914 0.39 -2.1 936,394 - Dshi_0914 0.59 +2.6 936,573 + Dshi_0914 0.78 -2.3 936,607 - Dshi_0914 0.81 -1.0 936,668 + Dshi_0914 0.87 -1.6
Or see this region's nucleotide sequence