Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0689 and Dshi_0690 overlap by 4 nucleotides Dshi_0690 and Dshi_0691 overlap by 4 nucleotides Dshi_0691 and Dshi_0692 overlap by 4 nucleotides
Dshi_0689: Dshi_0689 - isocitrate lyase family protein (RefSeq), at 694,835 to 695,713
_0689
Dshi_0690: Dshi_0690 - isochorismatase hydrolase (RefSeq), at 695,710 to 696,312
_0690
Dshi_0691: Dshi_0691 - 5-oxoprolinase (ATP-hydrolyzing) (RefSeq), at 696,309 to 698,378
_0691
Dshi_0692: Dshi_0692 - 5-oxoprolinase (ATP-hydrolyzing) (RefSeq), at 698,375 to 700,072
_0692
Position (kb) 696
697
698
699 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 695.323 kb on + strand, within Dshi_0689 at 695.367 kb on + strand, within Dshi_0689 at 695.375 kb on - strand, within Dshi_0689 at 695.375 kb on - strand, within Dshi_0689 at 695.377 kb on + strand, within Dshi_0689 at 695.435 kb on - strand, within Dshi_0689 at 695.458 kb on - strand, within Dshi_0689 at 695.492 kb on - strand, within Dshi_0689 at 695.545 kb on - strand, within Dshi_0689 at 695.566 kb on + strand, within Dshi_0689 at 695.568 kb on + strand, within Dshi_0689 at 695.593 kb on + strand, within Dshi_0689 at 695.767 kb on + strand at 695.775 kb on - strand, within Dshi_0690 at 695.775 kb on - strand, within Dshi_0690 at 695.793 kb on + strand, within Dshi_0690 at 695.852 kb on - strand, within Dshi_0690 at 695.861 kb on + strand, within Dshi_0690 at 695.944 kb on + strand, within Dshi_0690 at 695.944 kb on + strand, within Dshi_0690 at 695.952 kb on - strand, within Dshi_0690 at 695.957 kb on - strand, within Dshi_0690 at 695.994 kb on + strand, within Dshi_0690 at 696.001 kb on - strand, within Dshi_0690 at 696.042 kb on - strand, within Dshi_0690 at 696.232 kb on + strand, within Dshi_0690 at 696.236 kb on + strand, within Dshi_0690 at 696.340 kb on + strand at 696.369 kb on - strand at 696.421 kb on - strand at 696.447 kb on - strand at 696.447 kb on - strand at 696.544 kb on + strand, within Dshi_0691 at 696.622 kb on + strand, within Dshi_0691 at 696.628 kb on + strand, within Dshi_0691 at 696.628 kb on + strand, within Dshi_0691 at 696.708 kb on + strand at 696.729 kb on + strand, within Dshi_0691 at 696.755 kb on + strand, within Dshi_0691 at 696.953 kb on - strand, within Dshi_0691 at 696.962 kb on + strand, within Dshi_0691 at 696.988 kb on + strand, within Dshi_0691 at 696.998 kb on - strand, within Dshi_0691 at 696.998 kb on - strand, within Dshi_0691 at 697.116 kb on - strand, within Dshi_0691 at 697.216 kb on + strand, within Dshi_0691 at 697.321 kb on - strand, within Dshi_0691 at 697.377 kb on - strand, within Dshi_0691 at 697.417 kb on + strand, within Dshi_0691 at 697.440 kb on + strand, within Dshi_0691 at 697.448 kb on - strand, within Dshi_0691 at 697.448 kb on - strand, within Dshi_0691 at 697.476 kb on + strand, within Dshi_0691 at 697.500 kb on + strand, within Dshi_0691 at 697.608 kb on + strand, within Dshi_0691 at 697.635 kb on - strand, within Dshi_0691 at 697.663 kb on + strand, within Dshi_0691 at 697.707 kb on + strand, within Dshi_0691 at 697.707 kb on + strand, within Dshi_0691 at 697.721 kb on - strand, within Dshi_0691 at 697.785 kb on - strand, within Dshi_0691 at 697.851 kb on - strand, within Dshi_0691 at 697.946 kb on + strand, within Dshi_0691 at 697.977 kb on + strand, within Dshi_0691 at 698.072 kb on + strand, within Dshi_0691 at 698.074 kb on + strand, within Dshi_0691 at 698.080 kb on - strand, within Dshi_0691 at 698.144 kb on - strand, within Dshi_0691 at 698.237 kb on + strand at 698.422 kb on + strand at 698.489 kb on + strand at 698.757 kb on - strand, within Dshi_0692 at 698.825 kb on + strand at 698.928 kb on + strand, within Dshi_0692 at 698.936 kb on - strand, within Dshi_0692 at 698.936 kb on - strand, within Dshi_0692 at 698.949 kb on + strand, within Dshi_0692 at 698.975 kb on + strand, within Dshi_0692 at 699.101 kb on + strand, within Dshi_0692 at 699.107 kb on - strand, within Dshi_0692 at 699.117 kb on - strand, within Dshi_0692 at 699.195 kb on + strand, within Dshi_0692 at 699.213 kb on - strand, within Dshi_0692
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 695,323 + Dshi_0689 0.56 +2.2 695,367 + Dshi_0689 0.61 -1.4 695,375 - Dshi_0689 0.61 -0.1 695,375 - Dshi_0689 0.61 -3.3 695,377 + Dshi_0689 0.62 +0.0 695,435 - Dshi_0689 0.68 +2.8 695,458 - Dshi_0689 0.71 +1.2 695,492 - Dshi_0689 0.75 -1.1 695,545 - Dshi_0689 0.81 -2.4 695,566 + Dshi_0689 0.83 +1.2 695,568 + Dshi_0689 0.83 -0.4 695,593 + Dshi_0689 0.86 -0.4 695,767 + +1.8 695,775 - Dshi_0690 0.11 +2.8 695,775 - Dshi_0690 0.11 -3.0 695,793 + Dshi_0690 0.14 -1.4 695,852 - Dshi_0690 0.24 +1.2 695,861 + Dshi_0690 0.25 +1.2 695,944 + Dshi_0690 0.39 +0.2 695,944 + Dshi_0690 0.39 +1.2 695,952 - Dshi_0690 0.40 -0.8 695,957 - Dshi_0690 0.41 +0.1 695,994 + Dshi_0690 0.47 -0.8 696,001 - Dshi_0690 0.48 -2.5 696,042 - Dshi_0690 0.55 -0.8 696,232 + Dshi_0690 0.87 -1.1 696,236 + Dshi_0690 0.87 +0.1 696,340 + +1.2 696,369 - -1.1 696,421 - +2.2 696,447 - +1.2 696,447 - -1.3 696,544 + Dshi_0691 0.11 +1.9 696,622 + Dshi_0691 0.15 -0.4 696,628 + Dshi_0691 0.15 -1.6 696,628 + Dshi_0691 0.15 -0.4 696,708 + +1.2 696,729 + Dshi_0691 0.20 -0.4 696,755 + Dshi_0691 0.22 +1.2 696,953 - Dshi_0691 0.31 -1.4 696,962 + Dshi_0691 0.32 +1.2 696,988 + Dshi_0691 0.33 +3.8 696,998 - Dshi_0691 0.33 -0.8 696,998 - Dshi_0691 0.33 -1.1 697,116 - Dshi_0691 0.39 +1.2 697,216 + Dshi_0691 0.44 -2.5 697,321 - Dshi_0691 0.49 +2.2 697,377 - Dshi_0691 0.52 -0.1 697,417 + Dshi_0691 0.54 +0.4 697,440 + Dshi_0691 0.55 +1.2 697,448 - Dshi_0691 0.55 +1.2 697,448 - Dshi_0691 0.55 +2.6 697,476 + Dshi_0691 0.56 +1.2 697,500 + Dshi_0691 0.58 -0.4 697,608 + Dshi_0691 0.63 +1.9 697,635 - Dshi_0691 0.64 -0.8 697,663 + Dshi_0691 0.65 +3.8 697,707 + Dshi_0691 0.68 +2.2 697,707 + Dshi_0691 0.68 -0.2 697,721 - Dshi_0691 0.68 -1.6 697,785 - Dshi_0691 0.71 -2.1 697,851 - Dshi_0691 0.74 -1.6 697,946 + Dshi_0691 0.79 -0.4 697,977 + Dshi_0691 0.81 +3.2 698,072 + Dshi_0691 0.85 +2.2 698,074 + Dshi_0691 0.85 -3.2 698,080 - Dshi_0691 0.86 -0.4 698,144 - Dshi_0691 0.89 +0.5 698,237 + +0.2 698,422 + +1.5 698,489 + +0.4 698,757 - Dshi_0692 0.22 +1.8 698,825 + +1.2 698,928 + Dshi_0692 0.33 -1.0 698,936 - Dshi_0692 0.33 -2.3 698,936 - Dshi_0692 0.33 +0.2 698,949 + Dshi_0692 0.34 -2.1 698,975 + Dshi_0692 0.35 -2.6 699,101 + Dshi_0692 0.43 +1.2 699,107 - Dshi_0692 0.43 +0.2 699,117 - Dshi_0692 0.44 +3.2 699,195 + Dshi_0692 0.48 -0.4 699,213 - Dshi_0692 0.49 +0.3
Or see this region's nucleotide sequence