Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0536 and Dshi_0537 are separated by 29 nucleotides Dshi_0537 and Dshi_0538 are separated by 185 nucleotides Dshi_0538 and Dshi_0539 are separated by 13 nucleotides
Dshi_0536: Dshi_0536 - biotin/lipoyl attachment domain-containing protein (RefSeq), at 520,939 to 522,135
_0536
Dshi_0537: Dshi_0537 - carbohydrate kinase FGGY (RefSeq), at 522,165 to 523,658
_0537
Dshi_0538: Dshi_0538 - protein of unknown function DUF1045 (RefSeq), at 523,844 to 524,515
_0538
Dshi_0539: Dshi_0539 - aldo/keto reductase (RefSeq), at 524,529 to 525,467
_0539
Position (kb)
522
523
524 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 521.171 kb on + strand, within Dshi_0536 at 521.171 kb on + strand, within Dshi_0536 at 521.179 kb on - strand, within Dshi_0536 at 521.234 kb on + strand, within Dshi_0536 at 521.339 kb on + strand, within Dshi_0536 at 521.415 kb on + strand, within Dshi_0536 at 521.423 kb on - strand, within Dshi_0536 at 521.440 kb on + strand, within Dshi_0536 at 521.500 kb on - strand, within Dshi_0536 at 521.534 kb on + strand, within Dshi_0536 at 521.587 kb on - strand, within Dshi_0536 at 521.614 kb on - strand, within Dshi_0536 at 521.678 kb on + strand, within Dshi_0536 at 521.797 kb on - strand, within Dshi_0536 at 521.934 kb on - strand, within Dshi_0536 at 521.953 kb on + strand, within Dshi_0536 at 521.953 kb on + strand, within Dshi_0536 at 521.994 kb on + strand, within Dshi_0536 at 522.066 kb on + strand at 522.106 kb on - strand at 522.140 kb on + strand at 522.153 kb on - strand at 522.155 kb on + strand at 522.155 kb on + strand at 522.155 kb on + strand at 522.155 kb on + strand at 522.190 kb on + strand at 522.204 kb on - strand at 522.303 kb on + strand at 522.303 kb on + strand at 522.334 kb on - strand, within Dshi_0537 at 522.425 kb on + strand, within Dshi_0537 at 522.468 kb on + strand, within Dshi_0537 at 522.498 kb on + strand, within Dshi_0537 at 522.541 kb on + strand, within Dshi_0537 at 522.549 kb on - strand, within Dshi_0537 at 522.549 kb on - strand, within Dshi_0537 at 522.551 kb on + strand, within Dshi_0537 at 522.563 kb on + strand, within Dshi_0537 at 522.663 kb on - strand, within Dshi_0537 at 522.663 kb on - strand, within Dshi_0537 at 522.665 kb on + strand, within Dshi_0537 at 522.694 kb on - strand, within Dshi_0537 at 522.707 kb on - strand, within Dshi_0537 at 522.756 kb on + strand, within Dshi_0537 at 522.769 kb on - strand, within Dshi_0537 at 522.774 kb on - strand, within Dshi_0537 at 522.774 kb on - strand, within Dshi_0537 at 522.869 kb on + strand, within Dshi_0537 at 522.869 kb on + strand, within Dshi_0537 at 522.916 kb on + strand, within Dshi_0537 at 522.936 kb on + strand, within Dshi_0537 at 522.942 kb on - strand, within Dshi_0537 at 522.944 kb on - strand, within Dshi_0537 at 522.944 kb on - strand, within Dshi_0537 at 523.027 kb on - strand, within Dshi_0537 at 523.106 kb on + strand, within Dshi_0537 at 523.142 kb on + strand, within Dshi_0537 at 523.176 kb on - strand, within Dshi_0537 at 523.215 kb on + strand, within Dshi_0537 at 523.215 kb on + strand, within Dshi_0537 at 523.223 kb on - strand, within Dshi_0537 at 523.223 kb on - strand, within Dshi_0537 at 523.223 kb on - strand, within Dshi_0537 at 523.274 kb on + strand, within Dshi_0537 at 523.303 kb on - strand, within Dshi_0537 at 523.393 kb on + strand, within Dshi_0537 at 523.393 kb on + strand, within Dshi_0537 at 523.544 kb on + strand at 523.544 kb on + strand at 523.552 kb on - strand at 523.552 kb on - strand at 523.574 kb on + strand at 523.584 kb on + strand at 523.597 kb on - strand at 523.609 kb on - strand at 523.640 kb on - strand at 523.752 kb on - strand at 523.753 kb on - strand at 523.754 kb on + strand at 523.762 kb on - strand at 523.766 kb on - strand at 523.813 kb on + strand at 523.813 kb on - strand at 524.540 kb on - strand at 524.572 kb on + strand at 524.577 kb on + strand at 524.595 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 521,171 + Dshi_0536 0.19 -1.6 521,171 + Dshi_0536 0.19 +0.4 521,179 - Dshi_0536 0.20 -0.7 521,234 + Dshi_0536 0.25 +1.4 521,339 + Dshi_0536 0.33 +0.4 521,415 + Dshi_0536 0.40 +1.4 521,423 - Dshi_0536 0.40 +1.7 521,440 + Dshi_0536 0.42 +0.1 521,500 - Dshi_0536 0.47 +0.1 521,534 + Dshi_0536 0.50 -2.0 521,587 - Dshi_0536 0.54 +1.5 521,614 - Dshi_0536 0.56 +0.9 521,678 + Dshi_0536 0.62 +0.2 521,797 - Dshi_0536 0.72 +2.4 521,934 - Dshi_0536 0.83 -0.2 521,953 + Dshi_0536 0.85 +1.2 521,953 + Dshi_0536 0.85 +0.5 521,994 + Dshi_0536 0.88 +0.5 522,066 + -0.2 522,106 - -0.5 522,140 + +4.0 522,153 - +3.8 522,155 + -1.3 522,155 + -1.2 522,155 + -0.6 522,155 + -0.2 522,190 + -1.3 522,204 - +1.8 522,303 + +0.2 522,303 + -0.4 522,334 - Dshi_0537 0.11 +3.3 522,425 + Dshi_0537 0.17 +0.7 522,468 + Dshi_0537 0.20 +0.2 522,498 + Dshi_0537 0.22 -0.3 522,541 + Dshi_0537 0.25 +0.8 522,549 - Dshi_0537 0.26 -1.7 522,549 - Dshi_0537 0.26 -1.1 522,551 + Dshi_0537 0.26 +0.8 522,563 + Dshi_0537 0.27 +1.1 522,663 - Dshi_0537 0.33 +0.0 522,663 - Dshi_0537 0.33 +1.2 522,665 + Dshi_0537 0.33 +1.3 522,694 - Dshi_0537 0.35 +2.3 522,707 - Dshi_0537 0.36 -3.2 522,756 + Dshi_0537 0.40 +1.6 522,769 - Dshi_0537 0.40 +0.7 522,774 - Dshi_0537 0.41 +1.2 522,774 - Dshi_0537 0.41 +1.8 522,869 + Dshi_0537 0.47 -0.3 522,869 + Dshi_0537 0.47 +3.0 522,916 + Dshi_0537 0.50 +2.4 522,936 + Dshi_0537 0.52 +1.7 522,942 - Dshi_0537 0.52 -0.3 522,944 - Dshi_0537 0.52 +1.0 522,944 - Dshi_0537 0.52 +1.4 523,027 - Dshi_0537 0.58 +1.2 523,106 + Dshi_0537 0.63 -1.6 523,142 + Dshi_0537 0.65 +1.1 523,176 - Dshi_0537 0.68 +2.0 523,215 + Dshi_0537 0.70 +1.0 523,215 + Dshi_0537 0.70 -1.2 523,223 - Dshi_0537 0.71 +2.0 523,223 - Dshi_0537 0.71 +0.7 523,223 - Dshi_0537 0.71 +3.3 523,274 + Dshi_0537 0.74 -0.3 523,303 - Dshi_0537 0.76 -0.1 523,393 + Dshi_0537 0.82 +0.7 523,393 + Dshi_0537 0.82 +0.9 523,544 + +1.1 523,544 + +2.2 523,552 - -0.1 523,552 - -0.1 523,574 + -0.0 523,584 + +0.4 523,597 - -0.6 523,609 - +1.4 523,640 - +2.0 523,752 - -1.5 523,753 - -0.1 523,754 + +0.4 523,762 - -0.1 523,766 - -1.2 523,813 + +1.1 523,813 - +1.0 524,540 - +0.4 524,572 + +0.5 524,577 + +1.5 524,595 - -3.0
Or see this region's nucleotide sequence