Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0428 and Dshi_0429 are separated by 112 nucleotides Dshi_0429 and Dshi_0430 are separated by 22 nucleotides Dshi_0430 and Dshi_0431 are separated by 203 nucleotides
Dshi_0428: Dshi_0428 - Poly-beta-hydroxybutyrate polymerase domain protein (RefSeq), at 419,269 to 421,152
_0428
Dshi_0429: Dshi_0429 - uncharacterized conserved coiled coil protein (RefSeq), at 421,265 to 421,552
_0429
Dshi_0430: Dshi_0430 - hypothetical protein (RefSeq), at 421,575 to 422,009
_0430
Dshi_0431: Dshi_0431 - C4-dicarboxylate anaerobic carrier (RefSeq), at 422,213 to 423,661
_0431
Position (kb)
421
422 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 420.282 kb on + strand, within Dshi_0428 at 420.291 kb on + strand, within Dshi_0428 at 420.300 kb on + strand, within Dshi_0428 at 420.325 kb on + strand, within Dshi_0428 at 420.343 kb on - strand, within Dshi_0428 at 420.361 kb on - strand, within Dshi_0428 at 420.364 kb on - strand, within Dshi_0428 at 420.380 kb on + strand, within Dshi_0428 at 420.395 kb on + strand, within Dshi_0428 at 420.416 kb on + strand, within Dshi_0428 at 420.416 kb on + strand, within Dshi_0428 at 420.416 kb on - strand, within Dshi_0428 at 420.500 kb on - strand, within Dshi_0428 at 420.510 kb on + strand, within Dshi_0428 at 420.515 kb on - strand, within Dshi_0428 at 420.596 kb on + strand, within Dshi_0428 at 420.606 kb on - strand, within Dshi_0428 at 420.606 kb on - strand, within Dshi_0428 at 420.674 kb on - strand, within Dshi_0428 at 420.685 kb on - strand, within Dshi_0428 at 420.686 kb on + strand, within Dshi_0428 at 420.712 kb on - strand, within Dshi_0428 at 420.727 kb on + strand, within Dshi_0428 at 420.734 kb on + strand, within Dshi_0428 at 420.884 kb on + strand, within Dshi_0428 at 420.889 kb on + strand, within Dshi_0428 at 420.938 kb on - strand, within Dshi_0428 at 420.945 kb on + strand, within Dshi_0428 at 420.945 kb on + strand, within Dshi_0428 at 420.953 kb on - strand, within Dshi_0428 at 420.969 kb on + strand at 421.017 kb on - strand at 421.019 kb on + strand at 421.072 kb on - strand at 421.103 kb on - strand at 421.133 kb on + strand at 421.141 kb on - strand at 421.154 kb on - strand at 421.188 kb on + strand at 421.188 kb on + strand at 421.188 kb on + strand at 421.232 kb on + strand at 421.245 kb on - strand at 421.251 kb on - strand at 421.251 kb on - strand at 421.259 kb on - strand at 421.263 kb on + strand at 421.271 kb on - strand at 421.320 kb on + strand, within Dshi_0429 at 421.351 kb on - strand, within Dshi_0429 at 421.355 kb on + strand, within Dshi_0429 at 421.415 kb on - strand, within Dshi_0429 at 421.536 kb on + strand at 421.555 kb on + strand at 421.573 kb on + strand at 421.573 kb on + strand at 421.581 kb on - strand at 421.582 kb on + strand at 421.582 kb on + strand at 421.590 kb on - strand at 421.590 kb on - strand at 421.599 kb on - strand at 421.600 kb on + strand at 421.610 kb on - strand at 421.752 kb on - strand, within Dshi_0430 at 421.796 kb on - strand, within Dshi_0430 at 421.801 kb on - strand, within Dshi_0430 at 421.923 kb on - strand, within Dshi_0430 at 421.956 kb on + strand, within Dshi_0430 at 421.969 kb on - strand at 422.007 kb on - strand at 422.007 kb on - strand at 422.068 kb on + strand at 422.081 kb on - strand at 422.081 kb on - strand at 422.094 kb on + strand at 422.121 kb on + strand at 422.129 kb on - strand at 422.160 kb on - strand at 422.253 kb on + strand at 422.266 kb on + strand at 422.315 kb on + strand at 422.315 kb on + strand at 422.323 kb on - strand at 422.325 kb on + strand at 422.325 kb on + strand at 422.328 kb on - strand at 422.366 kb on + strand, within Dshi_0431 at 422.434 kb on - strand, within Dshi_0431 at 422.464 kb on + strand, within Dshi_0431 at 422.478 kb on + strand, within Dshi_0431 at 422.479 kb on - strand, within Dshi_0431 at 422.517 kb on - strand, within Dshi_0431
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 420,282 + Dshi_0428 0.54 +0.1 420,291 + Dshi_0428 0.54 +0.2 420,300 + Dshi_0428 0.55 -0.7 420,325 + Dshi_0428 0.56 +1.9 420,343 - Dshi_0428 0.57 -0.4 420,361 - Dshi_0428 0.58 -1.0 420,364 - Dshi_0428 0.58 +3.2 420,380 + Dshi_0428 0.59 +0.1 420,395 + Dshi_0428 0.60 -0.9 420,416 + Dshi_0428 0.61 +0.9 420,416 + Dshi_0428 0.61 -1.9 420,416 - Dshi_0428 0.61 +0.3 420,500 - Dshi_0428 0.65 +0.9 420,510 + Dshi_0428 0.66 -0.3 420,515 - Dshi_0428 0.66 -1.1 420,596 + Dshi_0428 0.70 -2.7 420,606 - Dshi_0428 0.71 -2.3 420,606 - Dshi_0428 0.71 +0.5 420,674 - Dshi_0428 0.75 -1.1 420,685 - Dshi_0428 0.75 -2.1 420,686 + Dshi_0428 0.75 -2.3 420,712 - Dshi_0428 0.77 +0.6 420,727 + Dshi_0428 0.77 +2.9 420,734 + Dshi_0428 0.78 -0.4 420,884 + Dshi_0428 0.86 -1.1 420,889 + Dshi_0428 0.86 +0.9 420,938 - Dshi_0428 0.89 -0.9 420,945 + Dshi_0428 0.89 -2.3 420,945 + Dshi_0428 0.89 -0.3 420,953 - Dshi_0428 0.89 -1.7 420,969 + -0.3 421,017 - +0.9 421,019 + -3.5 421,072 - -3.6 421,103 - +1.8 421,133 + -2.7 421,141 - -0.7 421,154 - -1.1 421,188 + -2.1 421,188 + -0.8 421,188 + -0.9 421,232 + +1.3 421,245 - -0.3 421,251 - -2.7 421,251 - -2.0 421,259 - -1.0 421,263 + -2.7 421,271 - +0.9 421,320 + Dshi_0429 0.19 -0.9 421,351 - Dshi_0429 0.30 -0.7 421,355 + Dshi_0429 0.31 +1.6 421,415 - Dshi_0429 0.52 -2.1 421,536 + +0.5 421,555 + -1.9 421,573 + -2.1 421,573 + +0.5 421,581 - -0.9 421,582 + +1.7 421,582 + +2.0 421,590 - -1.3 421,590 - +0.0 421,599 - -0.4 421,600 + -0.2 421,610 - -1.7 421,752 - Dshi_0430 0.41 -0.3 421,796 - Dshi_0430 0.51 -3.0 421,801 - Dshi_0430 0.52 -2.9 421,923 - Dshi_0430 0.80 -0.1 421,956 + Dshi_0430 0.88 -0.7 421,969 - -1.0 422,007 - -2.8 422,007 - -2.7 422,068 + -2.2 422,081 - -1.4 422,081 - -0.1 422,094 + -1.2 422,121 + -1.4 422,129 - -4.3 422,160 - +0.1 422,253 + +1.5 422,266 + +0.9 422,315 + +0.9 422,315 + -3.7 422,323 - -2.3 422,325 + -1.3 422,325 + -0.7 422,328 - +0.7 422,366 + Dshi_0431 0.11 -1.9 422,434 - Dshi_0431 0.15 +0.9 422,464 + Dshi_0431 0.17 -1.9 422,478 + Dshi_0431 0.18 -3.1 422,479 - Dshi_0431 0.18 -0.1 422,517 - Dshi_0431 0.21 +0.5
Or see this region's nucleotide sequence