Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0346 and Dshi_0347 are separated by 1 nucleotides Dshi_0347 and Dshi_0348 are separated by 28 nucleotides Dshi_0348 and Dshi_0349 are separated by 41 nucleotides Dshi_0349 and Dshi_0350 are separated by 120 nucleotides
Dshi_0346: Dshi_0346 - protein of unknown function DUF55 (RefSeq), at 332,071 to 332,535
_0346
Dshi_0347: Dshi_0347 - cob(II)yrinic acid a,c-diamide reductase (RefSeq), at 332,537 to 333,166
_0347
Dshi_0348: Dshi_0348 - ribosomal protein S16 (RefSeq), at 333,195 to 333,587
_0348
Dshi_0349: Dshi_0349 - chorismate mutase (RefSeq), at 333,629 to 333,925
_0349
Dshi_0350: Dshi_0350 - hypothetical protein (RefSeq), at 334,046 to 334,651
_0350
Position (kb) 333
334 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 332.314 kb on + strand, within Dshi_0346 at 332.407 kb on - strand, within Dshi_0346 at 332.419 kb on - strand, within Dshi_0346 at 332.621 kb on - strand, within Dshi_0347 at 332.636 kb on - strand, within Dshi_0347 at 332.724 kb on + strand, within Dshi_0347 at 332.724 kb on + strand, within Dshi_0347 at 332.769 kb on + strand, within Dshi_0347 at 332.804 kb on + strand, within Dshi_0347 at 332.881 kb on - strand, within Dshi_0347 at 333.077 kb on + strand, within Dshi_0347 at 333.282 kb on + strand, within Dshi_0348
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 332,314 + Dshi_0346 0.52 +4.3 332,407 - Dshi_0346 0.72 -2.8 332,419 - Dshi_0346 0.75 -2.0 332,621 - Dshi_0347 0.13 -1.3 332,636 - Dshi_0347 0.16 -1.3 332,724 + Dshi_0347 0.30 +0.2 332,724 + Dshi_0347 0.30 +1.2 332,769 + Dshi_0347 0.37 +1.2 332,804 + Dshi_0347 0.42 +1.2 332,881 - Dshi_0347 0.55 +0.2 333,077 + Dshi_0347 0.86 -0.9 333,282 + Dshi_0348 0.22 +1.2
Or see this region's nucleotide sequence