Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0218 and Dshi_0219 are separated by 3 nucleotides Dshi_0219 and Dshi_0220 are separated by 84 nucleotides Dshi_0220 and Dshi_0221 are separated by 93 nucleotides
Dshi_0218: Dshi_0218 - ATP--cobalamin adenosyltransferase (RefSeq), at 216,003 to 216,575
_0218
Dshi_0219: Dshi_0219 - hypothetical protein (RefSeq), at 216,579 to 216,794
_0219
Dshi_0220: Dshi_0220 - short-chain dehydrogenase/reductase SDR (RefSeq), at 216,879 to 217,712
_0220
Dshi_0221: Dshi_0221 - DNA topoisomerase IV, A subunit (RefSeq), at 217,806 to 220,139
_0221
Position (kb) 216
217
218 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 215.952 kb on - strand at 216.009 kb on - strand at 216.079 kb on - strand, within Dshi_0218 at 216.156 kb on - strand, within Dshi_0218 at 216.359 kb on - strand, within Dshi_0218 at 216.359 kb on - strand, within Dshi_0218 at 216.364 kb on - strand, within Dshi_0218 at 216.564 kb on + strand at 216.564 kb on + strand at 216.579 kb on - strand at 216.579 kb on - strand at 216.782 kb on - strand at 216.830 kb on + strand at 216.833 kb on - strand at 216.834 kb on + strand at 216.838 kb on - strand at 216.927 kb on - strand at 216.985 kb on - strand, within Dshi_0220 at 216.990 kb on - strand, within Dshi_0220 at 217.008 kb on - strand, within Dshi_0220 at 217.018 kb on + strand, within Dshi_0220 at 217.018 kb on + strand, within Dshi_0220 at 217.037 kb on + strand, within Dshi_0220 at 217.045 kb on - strand, within Dshi_0220 at 217.178 kb on + strand, within Dshi_0220 at 217.240 kb on - strand, within Dshi_0220 at 217.290 kb on - strand, within Dshi_0220 at 217.300 kb on - strand, within Dshi_0220 at 217.302 kb on + strand, within Dshi_0220 at 217.310 kb on - strand, within Dshi_0220 at 217.310 kb on - strand, within Dshi_0220 at 217.423 kb on + strand, within Dshi_0220 at 217.468 kb on + strand, within Dshi_0220 at 217.480 kb on + strand, within Dshi_0220 at 217.525 kb on + strand, within Dshi_0220 at 217.529 kb on + strand, within Dshi_0220 at 217.587 kb on - strand, within Dshi_0220 at 217.626 kb on - strand, within Dshi_0220 at 217.677 kb on + strand at 217.687 kb on + strand at 217.777 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 215,952 - -1.5 216,009 - +0.0 216,079 - Dshi_0218 0.13 +1.5 216,156 - Dshi_0218 0.27 -1.1 216,359 - Dshi_0218 0.62 -1.6 216,359 - Dshi_0218 0.62 +0.3 216,364 - Dshi_0218 0.63 -1.4 216,564 + -1.4 216,564 + -1.6 216,579 - +0.1 216,579 - -2.2 216,782 - -0.3 216,830 + +1.8 216,833 - -0.4 216,834 + +1.4 216,838 - +0.2 216,927 - -0.3 216,985 - Dshi_0220 0.13 -0.9 216,990 - Dshi_0220 0.13 +1.9 217,008 - Dshi_0220 0.15 +0.6 217,018 + Dshi_0220 0.17 +1.3 217,018 + Dshi_0220 0.17 +1.2 217,037 + Dshi_0220 0.19 +0.1 217,045 - Dshi_0220 0.20 -3.0 217,178 + Dshi_0220 0.36 -1.4 217,240 - Dshi_0220 0.43 +1.2 217,290 - Dshi_0220 0.49 +1.2 217,300 - Dshi_0220 0.50 -1.0 217,302 + Dshi_0220 0.51 -3.1 217,310 - Dshi_0220 0.52 -1.9 217,310 - Dshi_0220 0.52 +1.6 217,423 + Dshi_0220 0.65 -1.3 217,468 + Dshi_0220 0.71 -0.5 217,480 + Dshi_0220 0.72 +0.1 217,525 + Dshi_0220 0.77 -1.5 217,529 + Dshi_0220 0.78 +1.8 217,587 - Dshi_0220 0.85 +1.1 217,626 - Dshi_0220 0.90 +1.2 217,677 + -0.7 217,687 + +0.1 217,777 - -1.4
Or see this region's nucleotide sequence