Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0192 and Dshi_0193 are separated by 77 nucleotides Dshi_0193 and Dshi_0194 are separated by 4 nucleotides Dshi_0194 and Dshi_0195 are separated by 37 nucleotides Dshi_0195 and Dshi_0196 are separated by 25 nucleotides
Dshi_0192: Dshi_0192 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (RefSeq), at 192,409 to 192,978
_0192
Dshi_0193: Dshi_0193 - DNA-directed RNA polymerase, omega subunit (RefSeq), at 193,056 to 193,412
_0193
Dshi_0194: Dshi_0194 - (p)ppGpp synthetase I, SpoT/RelA (RefSeq), at 193,417 to 195,561
_0194
Dshi_0195: Dshi_0195 - hypothetical protein (RefSeq), at 195,599 to 196,255
_0195
Dshi_0196: Dshi_0196 - pyridoxal phosphate biosynthetic protein PdxJ (RefSeq), at 196,281 to 197,045
_0196
Position (kb) 193
194
195
196 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 193.396 kb on - strand at 193.546 kb on + strand at 193.728 kb on + strand, within Dshi_0194 at 193.803 kb on + strand, within Dshi_0194 at 193.826 kb on - strand, within Dshi_0194 at 193.992 kb on - strand, within Dshi_0194 at 194.013 kb on - strand, within Dshi_0194 at 194.077 kb on + strand, within Dshi_0194 at 194.345 kb on + strand, within Dshi_0194 at 194.356 kb on + strand, within Dshi_0194 at 194.376 kb on - strand, within Dshi_0194 at 194.464 kb on - strand, within Dshi_0194 at 194.574 kb on + strand, within Dshi_0194 at 194.609 kb on + strand, within Dshi_0194 at 194.615 kb on + strand, within Dshi_0194 at 194.648 kb on - strand, within Dshi_0194 at 194.697 kb on + strand, within Dshi_0194 at 194.709 kb on + strand, within Dshi_0194 at 194.717 kb on - strand, within Dshi_0194 at 194.748 kb on + strand, within Dshi_0194 at 194.879 kb on - strand, within Dshi_0194 at 194.880 kb on - strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.104 kb on + strand, within Dshi_0194 at 195.200 kb on + strand, within Dshi_0194 at 195.208 kb on - strand, within Dshi_0194 at 195.219 kb on - strand, within Dshi_0194 at 195.290 kb on + strand, within Dshi_0194 at 195.316 kb on + strand, within Dshi_0194 at 195.454 kb on + strand at 195.460 kb on - strand at 195.475 kb on - strand at 195.475 kb on - strand at 195.549 kb on - strand at 195.554 kb on + strand at 195.562 kb on - strand at 195.666 kb on - strand, within Dshi_0195 at 195.666 kb on - strand, within Dshi_0195 at 195.678 kb on + strand, within Dshi_0195 at 195.887 kb on - strand, within Dshi_0195 at 195.887 kb on - strand, within Dshi_0195 at 195.904 kb on + strand, within Dshi_0195 at 195.912 kb on - strand, within Dshi_0195 at 195.957 kb on + strand, within Dshi_0195 at 195.963 kb on - strand, within Dshi_0195 at 195.972 kb on + strand, within Dshi_0195 at 195.990 kb on - strand, within Dshi_0195 at 195.995 kb on + strand, within Dshi_0195 at 196.072 kb on + strand, within Dshi_0195 at 196.080 kb on - strand, within Dshi_0195 at 196.080 kb on - strand, within Dshi_0195 at 196.080 kb on - strand, within Dshi_0195 at 196.142 kb on - strand, within Dshi_0195 at 196.171 kb on + strand, within Dshi_0195 at 196.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 193,396 - +0.3 193,546 + -0.8 193,728 + Dshi_0194 0.14 +0.8 193,803 + Dshi_0194 0.18 +0.3 193,826 - Dshi_0194 0.19 +0.1 193,992 - Dshi_0194 0.27 -2.9 194,013 - Dshi_0194 0.28 -3.8 194,077 + Dshi_0194 0.31 -0.4 194,345 + Dshi_0194 0.43 -2.9 194,356 + Dshi_0194 0.44 -2.5 194,376 - Dshi_0194 0.45 -0.8 194,464 - Dshi_0194 0.49 -1.1 194,574 + Dshi_0194 0.54 -1.4 194,609 + Dshi_0194 0.56 -1.4 194,615 + Dshi_0194 0.56 +0.2 194,648 - Dshi_0194 0.57 -1.3 194,697 + Dshi_0194 0.60 +2.6 194,709 + Dshi_0194 0.60 +1.2 194,717 - Dshi_0194 0.61 -2.5 194,748 + Dshi_0194 0.62 -0.0 194,879 - Dshi_0194 0.68 +0.6 194,880 - Dshi_0194 0.68 +0.0 195,010 + Dshi_0194 0.74 +1.2 195,010 + Dshi_0194 0.74 -2.1 195,010 + Dshi_0194 0.74 -0.4 195,010 + Dshi_0194 0.74 +0.6 195,010 + Dshi_0194 0.74 -2.9 195,104 + Dshi_0194 0.79 +3.2 195,200 + Dshi_0194 0.83 +3.8 195,208 - Dshi_0194 0.83 +2.0 195,219 - Dshi_0194 0.84 -0.9 195,290 + Dshi_0194 0.87 +1.2 195,316 + Dshi_0194 0.89 +0.2 195,454 + +0.6 195,460 - -0.3 195,475 - -0.4 195,475 - -0.6 195,549 - +2.2 195,554 + -0.8 195,562 - -1.1 195,666 - Dshi_0195 0.10 +1.2 195,666 - Dshi_0195 0.10 +1.2 195,678 + Dshi_0195 0.12 +0.0 195,887 - Dshi_0195 0.44 +1.2 195,887 - Dshi_0195 0.44 +2.2 195,904 + Dshi_0195 0.46 -4.4 195,912 - Dshi_0195 0.48 +0.1 195,957 + Dshi_0195 0.54 +2.5 195,963 - Dshi_0195 0.55 -3.4 195,972 + Dshi_0195 0.57 +1.2 195,990 - Dshi_0195 0.60 -2.1 195,995 + Dshi_0195 0.60 +0.3 196,072 + Dshi_0195 0.72 -1.1 196,080 - Dshi_0195 0.73 -0.1 196,080 - Dshi_0195 0.73 +1.2 196,080 - Dshi_0195 0.73 +1.2 196,142 - Dshi_0195 0.83 -1.1 196,171 + Dshi_0195 0.87 -3.2 196,295 - -0.8
Or see this region's nucleotide sequence