Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3478 and rpmG are separated by 323 nucleotides rpmG and Shew_3480 are separated by 6 nucleotides Shew_3480 and Shew_3481 are separated by 123 nucleotides Shew_3481 and Shew_3482 are separated by 111 nucleotides
Shew_3478: Shew_3478 - N-acetylglutamate synthase (RefSeq), at 4,164,531 to 4,165,853
_3478
Shew_3479: rpmG - 50S ribosomal protein L33 (RefSeq), at 4,166,177 to 4,166,350
rpmG
Shew_3480: Shew_3480 - ribosomal protein L28 (RefSeq), at 4,166,357 to 4,166,593
_3480
Shew_3481: Shew_3481 - DNA repair protein RadC (RefSeq), at 4,166,717 to 4,167,418
_3481
Shew_3482: Shew_3482 - phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase (RefSeq), at 4,167,530 to 4,168,726
_3482
Position (kb)
4166
4167 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4165.452 kb on - strand, within Shew_3478 at 4165.480 kb on + strand, within Shew_3478 at 4165.650 kb on - strand, within Shew_3478 at 4165.814 kb on - strand at 4166.022 kb on + strand at 4166.056 kb on + strand at 4166.056 kb on + strand at 4166.073 kb on - strand at 4166.983 kb on + strand, within Shew_3481 at 4166.993 kb on + strand, within Shew_3481 at 4166.995 kb on + strand, within Shew_3481 at 4167.017 kb on + strand, within Shew_3481 at 4167.215 kb on - strand, within Shew_3481 at 4167.331 kb on - strand, within Shew_3481 at 4167.408 kb on - strand at 4167.432 kb on - strand at 4167.488 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 4,165,452 - Shew_3478 0.70 -2.7 4,165,480 + Shew_3478 0.72 -2.0 4,165,650 - Shew_3478 0.85 -5.2 4,165,814 - -4.6 4,166,022 + -0.6 4,166,056 + +0.0 4,166,056 + -0.8 4,166,073 - -0.6 4,166,983 + Shew_3481 0.38 +0.6 4,166,993 + Shew_3481 0.39 -0.6 4,166,995 + Shew_3481 0.40 +0.2 4,167,017 + Shew_3481 0.43 -4.1 4,167,215 - Shew_3481 0.71 -0.6 4,167,331 - Shew_3481 0.87 +0.4 4,167,408 - -3.5 4,167,432 - +1.5 4,167,488 + +0.1
Or see this region's nucleotide sequence