Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt napG and napH overlap by 4 nucleotides napH and Shew_3209 overlap by 4 nucleotides Shew_3209 and Shew_3210 are separated by 529 nucleotides Shew_3210 and Shew_3211 are separated by 52 nucleotides
Shew_3207: napG - quinol dehydrogenase periplasmic component (RefSeq), at 3,826,150 to 3,826,881
napG
Shew_3208: napH - quinol dehydrogenase membrane component (RefSeq), at 3,826,878 to 3,827,777
napH
Shew_3209: Shew_3209 - nitrate reductase cytochrome c-type subunit (NapB) (RefSeq), at 3,827,774 to 3,828,196
_3209
Shew_3210: Shew_3210 - hypothetical protein (RefSeq), at 3,828,726 to 3,829,013
_3210
Shew_3211: Shew_3211 - glutathione S-transferase domain-containing protein (RefSeq), at 3,829,066 to 3,829,662
_3211
Position (kb)
3827
3828
3829 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3826.776 kb on + strand, within napG at 3826.847 kb on + strand at 3827.151 kb on + strand, within napH at 3827.398 kb on - strand, within napH at 3827.503 kb on - strand, within napH at 3827.505 kb on + strand, within napH at 3827.538 kb on - strand, within napH at 3827.615 kb on + strand, within napH at 3827.911 kb on - strand, within Shew_3209 at 3827.969 kb on + strand, within Shew_3209 at 3828.642 kb on + strand at 3828.833 kb on + strand, within Shew_3210 at 3828.911 kb on - strand, within Shew_3210 at 3829.017 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 3,826,776 + napG Shew_3207 0.86 -3.0 3,826,847 + +0.5 3,827,151 + napH Shew_3208 0.30 -1.3 3,827,398 - napH Shew_3208 0.58 -2.5 3,827,503 - napH Shew_3208 0.69 +1.8 3,827,505 + napH Shew_3208 0.70 +0.6 3,827,538 - napH Shew_3208 0.73 -1.1 3,827,615 + napH Shew_3208 0.82 -0.8 3,827,911 - Shew_3209 0.32 +2.6 3,827,969 + Shew_3209 0.46 +0.6 3,828,642 + +0.1 3,828,833 + Shew_3210 0.37 +1.1 3,828,911 - Shew_3210 0.64 +0.7 3,829,017 + +0.9
Or see this region's nucleotide sequence