Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3128 and Shew_3129 are separated by 159 nucleotides Shew_3129 and Shew_3130 are separated by 50 nucleotides Shew_3130 and Shew_3131 are separated by 81 nucleotides
Shew_3128: Shew_3128 - sugar fermentation stimulation protein (RefSeq), at 3,720,572 to 3,721,276
_3128
Shew_3129: Shew_3129 - TraR/DksA family transcriptional regulator (RefSeq), at 3,721,436 to 3,721,879
_3129
Shew_3130: Shew_3130 - glutamate--tRNA ligase (RefSeq), at 3,721,930 to 3,722,889
_3130
Shew_3131: Shew_3131 - poly(A) polymerase (RefSeq), at 3,722,971 to 3,724,413
_3131
Position (kb)
3721
3722
3723 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3720.952 kb on + strand, within Shew_3128 at 3720.960 kb on - strand, within Shew_3128 at 3720.976 kb on + strand, within Shew_3128 at 3721.077 kb on + strand, within Shew_3128 at 3721.218 kb on + strand at 3722.022 kb on + strand at 3722.030 kb on - strand, within Shew_3130 at 3722.156 kb on + strand, within Shew_3130 at 3722.380 kb on + strand, within Shew_3130 at 3722.386 kb on - strand, within Shew_3130 at 3722.737 kb on + strand, within Shew_3130 at 3722.822 kb on - strand at 3722.901 kb on + strand at 3722.991 kb on + strand at 3723.576 kb on + strand, within Shew_3131
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 3,720,952 + Shew_3128 0.54 +0.7 3,720,960 - Shew_3128 0.55 -1.0 3,720,976 + Shew_3128 0.57 +1.4 3,721,077 + Shew_3128 0.72 +0.3 3,721,218 + +0.8 3,722,022 + -0.4 3,722,030 - Shew_3130 0.10 -1.9 3,722,156 + Shew_3130 0.24 +0.2 3,722,380 + Shew_3130 0.47 +0.1 3,722,386 - Shew_3130 0.47 +2.6 3,722,737 + Shew_3130 0.84 -0.2 3,722,822 - +1.3 3,722,901 + -1.9 3,722,991 + +2.3 3,723,576 + Shew_3131 0.42 +0.8
Or see this region's nucleotide sequence