Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2347 and Shew_2348 are separated by 239 nucleotides Shew_2348 and Shew_2349 overlap by 10 nucleotides Shew_2349 and Shew_2350 overlap by 4 nucleotides Shew_2350 and Shew_2351 are separated by 120 nucleotides
Shew_2347: Shew_2347 - phosphonate ABC transporter, periplasmic phosphonate-binding protein (RefSeq), at 2,741,403 to 2,742,257
_2347
Shew_2348: Shew_2348 - two component transcriptional regulator (RefSeq), at 2,742,497 to 2,743,171
_2348
Shew_2349: Shew_2349 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 2,743,162 to 2,744,511
_2349
Shew_2350: Shew_2350 - 2OG-Fe(II) oxygenase (RefSeq), at 2,744,508 to 2,745,203
_2350
Shew_2351: Shew_2351 - metal dependent phosphohydrolase (RefSeq), at 2,745,324 to 2,746,589
_2351
Position (kb)
2743
2744
2745 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2742.252 kb on + strand at 2742.394 kb on - strand at 2742.549 kb on - strand at 2743.389 kb on + strand, within Shew_2349 at 2743.397 kb on - strand, within Shew_2349 at 2743.487 kb on - strand, within Shew_2349 at 2743.866 kb on - strand, within Shew_2349 at 2744.124 kb on + strand, within Shew_2349 at 2745.041 kb on + strand, within Shew_2350 at 2745.132 kb on - strand, within Shew_2350 at 2745.407 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 2,742,252 + -3.5 2,742,394 - +0.8 2,742,549 - +1.4 2,743,389 + Shew_2349 0.17 +2.5 2,743,397 - Shew_2349 0.17 +3.9 2,743,487 - Shew_2349 0.24 +2.4 2,743,866 - Shew_2349 0.52 +1.9 2,744,124 + Shew_2349 0.71 +1.0 2,745,041 + Shew_2350 0.77 -0.2 2,745,132 - Shew_2350 0.90 -1.1 2,745,407 - -0.1
Or see this region's nucleotide sequence