Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1999 and Shew_R0065 are separated by 395 nucleotides Shew_R0065 and Shew_R0066 are separated by 38 nucleotides Shew_R0066 and Shew_R0067 are separated by 35 nucleotides Shew_R0067 and Shew_2000 are separated by 233 nucleotides Shew_2000 and Shew_2001 are separated by 236 nucleotides
Shew_1999: Shew_1999 - aromatic amino acid aminotransferase (RefSeq), at 2,310,338 to 2,311,531
_1999
Shew_R0065: Shew_R0065 - tRNA-Leu (RefSeq), at 2,311,927 to 2,312,013
_R0065
Shew_R0066: Shew_R0066 - tRNA-Cys (RefSeq), at 2,312,052 to 2,312,125
_R0066
Shew_R0067: Shew_R0067 - tRNA-Gly (RefSeq), at 2,312,161 to 2,312,236
_R0067
Shew_2000: Shew_2000 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,312,470 to 2,313,906
_2000
Shew_2001: Shew_2001 - hypothetical protein (RefSeq), at 2,314,143 to 2,314,949
_2001
Position (kb)
2312
2313
2314 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 2311.513 kb on - strand at 2311.574 kb on + strand at 2311.574 kb on + strand at 2312.691 kb on + strand, within Shew_2000 at 2313.180 kb on - strand, within Shew_2000 at 2313.922 kb on - strand at 2313.972 kb on + strand at 2313.972 kb on + strand at 2313.980 kb on - strand at 2314.130 kb on + strand at 2314.466 kb on + strand, within Shew_2001 at 2314.479 kb on + strand, within Shew_2001 at 2314.650 kb on - strand, within Shew_2001
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 2,311,513 - -0.8 2,311,574 + +2.2 2,311,574 + +1.2 2,312,691 + Shew_2000 0.15 -1.5 2,313,180 - Shew_2000 0.49 -0.7 2,313,922 - +6.5 2,313,972 + +0.8 2,313,972 + -2.4 2,313,980 - +2.5 2,314,130 + +3.2 2,314,466 + Shew_2001 0.40 +0.3 2,314,479 + Shew_2001 0.42 -1.0 2,314,650 - Shew_2001 0.63 -0.9
Or see this region's nucleotide sequence