Strain Fitness in Shewanella loihica PV-4 around Shew_2000

Experiment: Fumarate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_1999 and Shew_R0065 are separated by 395 nucleotidesShew_R0065 and Shew_R0066 are separated by 38 nucleotidesShew_R0066 and Shew_R0067 are separated by 35 nucleotidesShew_R0067 and Shew_2000 are separated by 233 nucleotidesShew_2000 and Shew_2001 are separated by 236 nucleotides Shew_1999: Shew_1999 - aromatic amino acid aminotransferase (RefSeq), at 2,310,338 to 2,311,531 _1999 Shew_R0065: Shew_R0065 - tRNA-Leu (RefSeq), at 2,311,927 to 2,312,013 _R0065 Shew_R0066: Shew_R0066 - tRNA-Cys (RefSeq), at 2,312,052 to 2,312,125 _R0066 Shew_R0067: Shew_R0067 - tRNA-Gly (RefSeq), at 2,312,161 to 2,312,236 _R0067 Shew_2000: Shew_2000 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,312,470 to 2,313,906 _2000 Shew_2001: Shew_2001 - hypothetical protein (RefSeq), at 2,314,143 to 2,314,949 _2001 Position (kb) 2312 2313 2314Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6at 2311.513 kb on - strandat 2311.574 kb on + strandat 2311.574 kb on + strandat 2312.691 kb on + strand, within Shew_2000at 2313.180 kb on - strand, within Shew_2000at 2313.922 kb on - strandat 2313.972 kb on + strandat 2313.972 kb on + strandat 2313.980 kb on - strandat 2314.130 kb on + strandat 2314.466 kb on + strand, within Shew_2001at 2314.479 kb on + strand, within Shew_2001at 2314.650 kb on - strand, within Shew_2001

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Per-strain Table

Position Strand Gene LocusTag Fraction Fumarate (C)
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2,311,513 - -0.8
2,311,574 + +2.2
2,311,574 + +1.2
2,312,691 + Shew_2000 0.15 -1.5
2,313,180 - Shew_2000 0.49 -0.7
2,313,922 - +6.5
2,313,972 + +0.8
2,313,972 + -2.4
2,313,980 - +2.5
2,314,130 + +3.2
2,314,466 + Shew_2001 0.40 +0.3
2,314,479 + Shew_2001 0.42 -1.0
2,314,650 - Shew_2001 0.63 -0.9

Or see this region's nucleotide sequence