Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1961 and Shew_1962 are separated by 107 nucleotides Shew_1962 and Shew_1963 are separated by 34 nucleotides Shew_1963 and Shew_1964 are separated by 230 nucleotides Shew_1964 and pssA are separated by 11 nucleotides
Shew_1961: Shew_1961 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 2,265,581 to 2,267,332
_1961
Shew_1962: Shew_1962 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase (RefSeq), at 2,267,440 to 2,269,071
_1962
Shew_1963: Shew_1963 - hypothetical protein (RefSeq), at 2,269,106 to 2,269,282
_1963
Shew_1964: Shew_1964 - hypothetical protein (RefSeq), at 2,269,513 to 2,269,845
_1964
Shew_1965: pssA - phosphatidylserine synthase (RefSeq), at 2,269,857 to 2,271,170
pssA
Position (kb)
2267
2268
2269
2270 Strain fitness (log2 ratio)
-2
-1
0
1 at 2266.496 kb on - strand, within Shew_1961 at 2267.254 kb on + strand at 2267.262 kb on - strand at 2267.897 kb on - strand, within Shew_1962 at 2267.978 kb on + strand, within Shew_1962 at 2268.746 kb on - strand, within Shew_1962 at 2269.283 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 2,266,496 - Shew_1961 0.52 -0.6 2,267,254 + +0.2 2,267,262 - -2.5 2,267,897 - Shew_1962 0.28 -1.8 2,267,978 + Shew_1962 0.33 -2.0 2,268,746 - Shew_1962 0.80 +0.1 2,269,283 + +0.8
Or see this region's nucleotide sequence