Experiment: acetate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_303 and HP15_304 are separated by 86 nucleotides HP15_304 and HP15_305 are separated by 172 nucleotides HP15_305 and HP15_306 are separated by 78 nucleotides HP15_306 and HP15_307 are separated by 61 nucleotides
HP15_303: HP15_303 - guanylate kinase, at 293,857 to 294,489
_303
HP15_304: HP15_304 - DNA-directed RNA polymerase subunit omega, at 294,576 to 294,788
_304
HP15_305: HP15_305 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 294,961 to 297,102
_305
HP15_306: HP15_306 - YjgF-like protein, at 297,181 to 297,567
_306
HP15_307: HP15_307 - NAD-dependent epimerase/dehydratase, at 297,629 to 298,495
_307
Position (kb)
294
295
296
297
298 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 294.764 kb on + strand, within HP15_304 at 296.603 kb on + strand, within HP15_305 at 296.603 kb on + strand, within HP15_305 at 296.603 kb on + strand, within HP15_305 at 296.797 kb on - strand, within HP15_305 at 296.877 kb on + strand, within HP15_305 at 296.877 kb on + strand, within HP15_305 at 297.010 kb on + strand at 297.044 kb on - strand at 297.265 kb on - strand, within HP15_306 at 297.357 kb on + strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.430 kb on - strand, within HP15_306 at 297.488 kb on - strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.627 kb on + strand at 297.698 kb on + strand at 297.805 kb on + strand, within HP15_307 at 297.813 kb on - strand, within HP15_307 at 297.845 kb on + strand, within HP15_307 at 297.850 kb on + strand, within HP15_307 at 297.906 kb on - strand, within HP15_307 at 297.908 kb on + strand, within HP15_307 at 297.934 kb on + strand, within HP15_307 at 297.964 kb on + strand, within HP15_307 at 298.080 kb on + strand, within HP15_307
Per-strain Table
Position Strand Gene LocusTag Fraction acetate remove 294,764 + HP15_304 0.88 -1.2 296,603 + HP15_305 0.77 -0.3 296,603 + HP15_305 0.77 -0.5 296,603 + HP15_305 0.77 -0.3 296,797 - HP15_305 0.86 -1.0 296,877 + HP15_305 0.89 -2.9 296,877 + HP15_305 0.89 -2.7 297,010 + -0.6 297,044 - -1.3 297,265 - HP15_306 0.22 +0.4 297,357 + HP15_306 0.45 +0.0 297,365 - HP15_306 0.48 -1.5 297,365 - HP15_306 0.48 -0.7 297,365 - HP15_306 0.48 +0.8 297,365 - HP15_306 0.48 -1.4 297,365 - HP15_306 0.48 +0.8 297,365 - HP15_306 0.48 -0.1 297,365 - HP15_306 0.48 +0.0 297,430 - HP15_306 0.64 -0.6 297,488 - HP15_306 0.79 +0.4 297,519 + HP15_306 0.87 -1.2 297,519 + HP15_306 0.87 +0.0 297,519 + HP15_306 0.87 -1.2 297,519 + HP15_306 0.87 -2.0 297,519 + HP15_306 0.87 -1.1 297,527 - HP15_306 0.89 -0.3 297,527 - HP15_306 0.89 +0.3 297,527 - HP15_306 0.89 -0.9 297,527 - HP15_306 0.89 +0.5 297,527 - HP15_306 0.89 -1.7 297,627 + +1.0 297,698 + -3.0 297,805 + HP15_307 0.20 -0.6 297,813 - HP15_307 0.21 -1.2 297,845 + HP15_307 0.25 -0.4 297,850 + HP15_307 0.25 -0.8 297,906 - HP15_307 0.32 +0.8 297,908 + HP15_307 0.32 +0.3 297,934 + HP15_307 0.35 -0.1 297,964 + HP15_307 0.39 -0.9 298,080 + HP15_307 0.52 -0.5
Or see this region's nucleotide sequence