Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3363 and Shew_3364 are separated by 6 nucleotides Shew_3364 and Shew_3365 are separated by 40 nucleotides Shew_3365 and Shew_3366 are separated by 70 nucleotides
Shew_3363: Shew_3363 - 3-dehydroquinate dehydratase, type II (RefSeq), at 4,010,975 to 4,011,427
_3363
Shew_3364: Shew_3364 - acetyl-CoA carboxylase, biotin carboxyl carrier protein (RefSeq), at 4,011,434 to 4,011,865
_3364
Shew_3365: Shew_3365 - acetyl-CoA carboxylase, biotin carboxylase (RefSeq), at 4,011,906 to 4,013,258
_3365
Shew_3366: Shew_3366 - metal dependent phosphohydrolase (RefSeq), at 4,013,329 to 4,016,526
_3366
Position (kb)
4011
4012
4013
4014 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4011.162 kb on + strand, within Shew_3363 at 4011.252 kb on - strand, within Shew_3363 at 4011.380 kb on + strand, within Shew_3363 at 4011.393 kb on - strand at 4011.403 kb on + strand at 4011.708 kb on - strand, within Shew_3364 at 4011.745 kb on - strand, within Shew_3364 at 4012.438 kb on - strand, within Shew_3365 at 4012.438 kb on - strand, within Shew_3365 at 4012.522 kb on - strand, within Shew_3365 at 4012.842 kb on - strand, within Shew_3365 at 4013.103 kb on - strand, within Shew_3365 at 4013.122 kb on - strand, within Shew_3365 at 4013.171 kb on + strand at 4013.174 kb on - strand at 4013.179 kb on - strand at 4013.179 kb on - strand at 4013.405 kb on - strand at 4013.405 kb on - strand at 4013.473 kb on - strand at 4013.799 kb on - strand, within Shew_3366 at 4013.852 kb on - strand, within Shew_3366 at 4014.116 kb on + strand, within Shew_3366 at 4014.119 kb on - strand, within Shew_3366 at 4014.126 kb on - strand, within Shew_3366 at 4014.154 kb on - strand, within Shew_3366 at 4014.200 kb on - strand, within Shew_3366
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 4,011,162 + Shew_3363 0.41 +2.9 4,011,252 - Shew_3363 0.61 +2.2 4,011,380 + Shew_3363 0.89 +2.4 4,011,393 - +2.1 4,011,403 + +0.6 4,011,708 - Shew_3364 0.63 -0.4 4,011,745 - Shew_3364 0.72 +0.6 4,012,438 - Shew_3365 0.39 +0.2 4,012,438 - Shew_3365 0.39 +0.1 4,012,522 - Shew_3365 0.46 -0.4 4,012,842 - Shew_3365 0.69 +1.2 4,013,103 - Shew_3365 0.88 -0.2 4,013,122 - Shew_3365 0.90 +1.5 4,013,171 + +0.2 4,013,174 - -2.5 4,013,179 - -0.1 4,013,179 - -0.2 4,013,405 - -0.0 4,013,405 - +0.5 4,013,473 - +1.1 4,013,799 - Shew_3366 0.15 -0.9 4,013,852 - Shew_3366 0.16 +0.2 4,014,116 + Shew_3366 0.25 -0.4 4,014,119 - Shew_3366 0.25 +0.8 4,014,126 - Shew_3366 0.25 +0.2 4,014,154 - Shew_3366 0.26 +1.1 4,014,200 - Shew_3366 0.27 +0.3
Or see this region's nucleotide sequence