Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt caiC and Shew_2674 are separated by 8 nucleotides Shew_2674 and Shew_2675 are separated by 160 nucleotides
Shew_2673: caiC - putative crotonobetaine/carnitine-CoA ligase (RefSeq), at 3,163,503 to 3,165,059
caiC
Shew_2674: Shew_2674 - crotonobetainyl-CoA:carnitine CoA-transferase (RefSeq), at 3,165,068 to 3,166,294
_2674
Shew_2675: Shew_2675 - crotonobetainyl-CoA dehydrogenase (RefSeq), at 3,166,455 to 3,167,591
_2675
Position (kb)
3165
3166
3167 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3164.199 kb on + strand, within caiC at 3164.413 kb on + strand, within caiC at 3164.731 kb on + strand, within caiC at 3164.747 kb on + strand, within caiC at 3164.755 kb on - strand, within caiC at 3164.856 kb on - strand, within caiC at 3165.410 kb on + strand, within Shew_2674 at 3165.418 kb on - strand, within Shew_2674 at 3165.534 kb on + strand, within Shew_2674 at 3165.534 kb on + strand, within Shew_2674 at 3165.578 kb on - strand, within Shew_2674 at 3165.662 kb on + strand, within Shew_2674 at 3165.687 kb on - strand, within Shew_2674 at 3165.862 kb on - strand, within Shew_2674 at 3165.940 kb on + strand, within Shew_2674 at 3165.948 kb on - strand, within Shew_2674 at 3165.948 kb on - strand, within Shew_2674 at 3165.948 kb on - strand, within Shew_2674 at 3165.953 kb on - strand, within Shew_2674 at 3165.994 kb on - strand, within Shew_2674 at 3166.196 kb on + strand at 3166.199 kb on - strand at 3166.743 kb on - strand, within Shew_2675 at 3166.766 kb on + strand, within Shew_2675 at 3166.857 kb on + strand, within Shew_2675 at 3166.957 kb on - strand, within Shew_2675 at 3167.198 kb on + strand, within Shew_2675
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 3,164,199 + caiC Shew_2673 0.45 -0.0 3,164,413 + caiC Shew_2673 0.58 -0.5 3,164,731 + caiC Shew_2673 0.79 -0.9 3,164,747 + caiC Shew_2673 0.80 +0.4 3,164,755 - caiC Shew_2673 0.80 +3.3 3,164,856 - caiC Shew_2673 0.87 -0.0 3,165,410 + Shew_2674 0.28 +0.3 3,165,418 - Shew_2674 0.29 -1.9 3,165,534 + Shew_2674 0.38 -0.3 3,165,534 + Shew_2674 0.38 -0.0 3,165,578 - Shew_2674 0.42 -0.6 3,165,662 + Shew_2674 0.48 -0.1 3,165,687 - Shew_2674 0.50 +0.4 3,165,862 - Shew_2674 0.65 +0.2 3,165,940 + Shew_2674 0.71 +0.3 3,165,948 - Shew_2674 0.72 +0.4 3,165,948 - Shew_2674 0.72 -0.2 3,165,948 - Shew_2674 0.72 -1.2 3,165,953 - Shew_2674 0.72 -0.1 3,165,994 - Shew_2674 0.75 -2.1 3,166,196 + -0.3 3,166,199 - +0.5 3,166,743 - Shew_2675 0.25 -0.5 3,166,766 + Shew_2675 0.27 -0.9 3,166,857 + Shew_2675 0.35 -0.0 3,166,957 - Shew_2675 0.44 +1.4 3,167,198 + Shew_2675 0.65 +0.1
Or see this region's nucleotide sequence