Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2445 and Shew_2446 are separated by 126 nucleotides Shew_2446 and Shew_2447 are separated by 234 nucleotides Shew_2447 and nrdG are separated by 158 nucleotides nrdG and Shew_2449 are separated by 121 nucleotides
Shew_2445: Shew_2445 - hypothetical protein (RefSeq), at 2,875,132 to 2,875,608
_2445
Shew_2446: Shew_2446 - GTP cyclohydrolase II (RefSeq), at 2,875,735 to 2,876,352
_2446
Shew_2447: Shew_2447 - hypothetical protein (RefSeq), at 2,876,587 to 2,876,943
_2447
Shew_2448: nrdG - anaerobic ribonucleotide reductase-activating protein (RefSeq), at 2,877,102 to 2,877,575
nrdG
Shew_2449: Shew_2449 - anaerobic ribonucleoside triphosphate reductase (RefSeq), at 2,877,697 to 2,879,814
_2449
Position (kb)
2876
2877 Strain fitness (log2 ratio)
-1
0
1 at 2876.623 kb on - strand, within Shew_2447 at 2876.719 kb on + strand, within Shew_2447 at 2876.722 kb on - strand, within Shew_2447 at 2876.739 kb on - strand, within Shew_2447 at 2876.752 kb on - strand, within Shew_2447 at 2877.294 kb on - strand, within nrdG at 2877.690 kb on - strand at 2877.783 kb on + strand at 2877.810 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 2,876,623 - Shew_2447 0.10 +0.3 2,876,719 + Shew_2447 0.37 +0.3 2,876,722 - Shew_2447 0.38 +0.9 2,876,739 - Shew_2447 0.43 +0.1 2,876,752 - Shew_2447 0.46 -0.1 2,877,294 - nrdG Shew_2448 0.41 +0.1 2,877,690 - +0.2 2,877,783 + +0.6 2,877,810 + +0.6
Or see this region's nucleotide sequence