Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2037 and Shew_R0069 are separated by 230 nucleotides Shew_R0069 and Shew_R0070 are separated by 20 nucleotides Shew_R0070 and Shew_2038 are separated by 61 nucleotides Shew_2038 and Shew_2039 are separated by 293 nucleotides
Shew_2037: Shew_2037 - MATE efflux family protein (RefSeq), at 2,348,979 to 2,350,364
_2037
Shew_R0069: Shew_R0069 - tRNA-Val (RefSeq), at 2,350,595 to 2,350,671
_R0069
Shew_R0070: Shew_R0070 - tRNA-Val (RefSeq), at 2,350,692 to 2,350,768
_R0070
Shew_2038: Shew_2038 - hypothetical protein (RefSeq), at 2,350,830 to 2,351,273
_2038
Shew_2039: Shew_2039 - GCN5-related N-acetyltransferase (RefSeq), at 2,351,567 to 2,354,269
_2039
Position (kb)
2350
2351
2352 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2349.848 kb on + strand, within Shew_2037 at 2349.940 kb on + strand, within Shew_2037 at 2350.141 kb on + strand, within Shew_2037 at 2350.267 kb on + strand at 2350.270 kb on - strand at 2351.032 kb on - strand, within Shew_2038 at 2352.080 kb on + strand, within Shew_2039 at 2352.088 kb on - strand, within Shew_2039
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 2,349,848 + Shew_2037 0.63 -0.7 2,349,940 + Shew_2037 0.69 +0.3 2,350,141 + Shew_2037 0.84 -0.8 2,350,267 + -0.5 2,350,270 - -0.6 2,351,032 - Shew_2038 0.45 -3.0 2,352,080 + Shew_2039 0.19 +0.1 2,352,088 - Shew_2039 0.19 +1.4
Or see this region's nucleotide sequence