Strain Fitness in Shewanella loihica PV-4 around Shew_0521

Experiment: Cephalothin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_0520 and metL are separated by 19 nucleotidesmetL and metF are separated by 263 nucleotides Shew_0520: Shew_0520 - O-succinylhomoserine (thiol)-lyase (RefSeq), at 589,176 to 590,342 _0520 Shew_0521: metL - bifunctional aspartate kinase II/homoserine dehydrogenase II (RefSeq), at 590,362 to 592,755 metL Shew_0522: metF - 5,10-methylenetetrahydrofolate reductase (RefSeq), at 593,019 to 593,909 metF Position (kb) 590 591 592 593Strain fitness (log2 ratio) -2 -1 0 1at 589.444 kb on - strand, within Shew_0520at 589.609 kb on - strand, within Shew_0520at 589.711 kb on - strand, within Shew_0520at 589.794 kb on - strand, within Shew_0520at 589.858 kb on + strand, within Shew_0520at 589.866 kb on - strand, within Shew_0520at 591.446 kb on - strand, within metLat 591.553 kb on + strand, within metLat 592.082 kb on - strand, within metLat 592.169 kb on + strand, within metLat 592.169 kb on + strand, within metLat 592.177 kb on - strand, within metLat 592.204 kb on - strand, within metLat 592.540 kb on - strandat 592.548 kb on + strandat 593.299 kb on - strand, within metFat 593.392 kb on + strand, within metFat 593.392 kb on + strand, within metFat 593.392 kb on + strand, within metFat 593.392 kb on + strand, within metFat 593.392 kb on + strand, within metFat 593.392 kb on + strand, within metFat 593.392 kb on + strand, within metFat 593.400 kb on - strand, within metFat 593.400 kb on - strand, within metFat 593.400 kb on - strand, within metFat 593.400 kb on - strand, within metFat 593.446 kb on - strand, within metFat 593.746 kb on + strand, within metF

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml
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589,444 - Shew_0520 0.23 +0.0
589,609 - Shew_0520 0.37 +0.5
589,711 - Shew_0520 0.46 -0.0
589,794 - Shew_0520 0.53 -0.3
589,858 + Shew_0520 0.58 -0.2
589,866 - Shew_0520 0.59 -0.8
591,446 - metL Shew_0521 0.45 -0.2
591,553 + metL Shew_0521 0.50 -2.2
592,082 - metL Shew_0521 0.72 -1.1
592,169 + metL Shew_0521 0.75 -0.0
592,169 + metL Shew_0521 0.75 -0.2
592,177 - metL Shew_0521 0.76 -2.0
592,204 - metL Shew_0521 0.77 +1.0
592,540 - -2.0
592,548 + -2.4
593,299 - metF Shew_0522 0.31 -0.0
593,392 + metF Shew_0522 0.42 +0.0
593,392 + metF Shew_0522 0.42 -0.2
593,392 + metF Shew_0522 0.42 -0.2
593,392 + metF Shew_0522 0.42 +0.1
593,392 + metF Shew_0522 0.42 -0.1
593,392 + metF Shew_0522 0.42 +0.1
593,392 + metF Shew_0522 0.42 -0.2
593,400 - metF Shew_0522 0.43 +0.2
593,400 - metF Shew_0522 0.43 +0.3
593,400 - metF Shew_0522 0.43 -0.3
593,400 - metF Shew_0522 0.43 +1.4
593,446 - metF Shew_0522 0.48 +0.1
593,746 + metF Shew_0522 0.82 +0.7

Or see this region's nucleotide sequence