Strain Fitness in Shewanella oneidensis MR-1 around SO2218

Experiment: Cobalt chloride 0.04 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntddlA and asnS are separated by 244 nucleotidesasnS and SO2219 are separated by 195 nucleotidesSO2219 and SO2220 are separated by 17 nucleotides SO2217: ddlA - D-alanine--D-alanine ligase (NCBI ptt file), at 2,331,687 to 2,332,697 ddlA SO2218: asnS - asparaginyl-tRNA synthetase (NCBI ptt file), at 2,332,942 to 2,334,342 asnS SO2219: SO2219 - hypothetical protein (NCBI ptt file), at 2,334,538 to 2,334,879 SO2219 SO2220: SO2220 - MutT/nudix family protein (NCBI ptt file), at 2,334,897 to 2,335,484 SO2220 Position (kb) 2332 2333 2334 2335Strain fitness (log2 ratio) -2 -1 0 1at 2332.883 kb on - strandat 2332.883 kb on - strandat 2334.453 kb on - strandat 2334.472 kb on - strandat 2334.493 kb on + strandat 2334.638 kb on - strand, within SO2219at 2334.644 kb on - strand, within SO2219at 2334.685 kb on - strand, within SO2219at 2334.789 kb on - strand, within SO2219at 2334.913 kb on + strandat 2334.918 kb on + strandat 2335.000 kb on + strand, within SO2220at 2335.013 kb on - strand, within SO2220at 2335.257 kb on - strand, within SO2220

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.04 mM
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2,332,883 - -0.2
2,332,883 - +0.1
2,334,453 - +1.3
2,334,472 - -0.3
2,334,493 + +0.0
2,334,638 - SO2219 0.29 +0.1
2,334,644 - SO2219 0.31 +0.2
2,334,685 - SO2219 0.43 -0.5
2,334,789 - SO2219 0.73 -0.2
2,334,913 + +0.8
2,334,918 + -0.1
2,335,000 + SO2220 0.18 -2.3
2,335,013 - SO2220 0.20 -0.6
2,335,257 - SO2220 0.61 -0.9

Or see this region's nucleotide sequence