Experiment: Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nqrC-2 and nqrD-2 overlap by 1 nucleotides nqrD-2 and nqrE-2 are separated by 5 nucleotides nqrE-2 and nqrF-2 are separated by 46 nucleotides
SO1105: nqrC-2 - NADH:ubiquinone oxidoreductase, Na translocating, gamma subunit (NCBI ptt file), at 1,148,762 to 1,149,550
nqrC-2
SO1106: nqrD-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 1,149,550 to 1,150,182
nqrD-2
SO1107: nqrE-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrE (NCBI ptt file), at 1,150,188 to 1,150,796
nqrE-2
SO1108: nqrF-2 - NADH:ubiquinone oxidoreductase, Na translocating, beta subunit (NCBI ptt file), at 1,150,843 to 1,152,099
nqrF-2
Position (kb)
1150
1151 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1149.336 kb on - strand, within nqrC-2 at 1149.408 kb on - strand, within nqrC-2 at 1149.774 kb on - strand, within nqrD-2 at 1150.157 kb on - strand at 1150.323 kb on + strand, within nqrE-2 at 1150.427 kb on - strand, within nqrE-2 at 1150.539 kb on + strand, within nqrE-2 at 1150.556 kb on - strand, within nqrE-2 at 1150.638 kb on + strand, within nqrE-2
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.04 mM remove 1,149,336 - nqrC-2 SO1105 0.73 -3.3 1,149,408 - nqrC-2 SO1105 0.82 -1.8 1,149,774 - nqrD-2 SO1106 0.35 -2.5 1,150,157 - -2.5 1,150,323 + nqrE-2 SO1107 0.22 -4.6 1,150,427 - nqrE-2 SO1107 0.39 -2.1 1,150,539 + nqrE-2 SO1107 0.58 -4.4 1,150,556 - nqrE-2 SO1107 0.60 -3.3 1,150,638 + nqrE-2 SO1107 0.74 -2.2
Or see this region's nucleotide sequence