Experiment: Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gppA and SO0409 are separated by 99 nucleotides SO0409 and mutT are separated by 138 nucleotides mutT and SO0411 are separated by 87 nucleotides SO0411 and SO0412 are separated by 60 nucleotides
SO0408: gppA - guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (NCBI ptt file), at 425,050 to 425,970
gppA
SO0409: SO0409 - hypothetical protein (NCBI ptt file), at 426,070 to 426,606
SO0409
SO0410: mutT - mutator mutT protein (NCBI ptt file), at 426,745 to 427,137
mutT
SO0411: SO0411 - conserved hypothetical protein (NCBI ptt file), at 427,225 to 427,434
SO0411
SO0412: SO0412 - conserved hypothetical protein (NCBI ptt file), at 427,495 to 428,229
SO0412
Position (kb)
426
427
428 Strain fitness (log2 ratio)
-1
0
1
2 at 425.966 kb on + strand at 426.044 kb on - strand at 426.118 kb on - strand at 426.156 kb on + strand, within SO0409 at 426.162 kb on + strand, within SO0409 at 426.164 kb on - strand, within SO0409 at 426.181 kb on + strand, within SO0409 at 426.356 kb on + strand, within SO0409 at 426.369 kb on + strand, within SO0409 at 426.369 kb on + strand, within SO0409 at 426.369 kb on + strand, within SO0409 at 426.369 kb on + strand, within SO0409 at 426.369 kb on + strand, within SO0409 at 426.377 kb on - strand, within SO0409 at 426.377 kb on - strand, within SO0409 at 426.447 kb on - strand, within SO0409 at 426.593 kb on + strand at 426.719 kb on + strand at 426.849 kb on + strand, within mutT at 426.982 kb on + strand, within mutT at 427.050 kb on + strand, within mutT at 427.060 kb on - strand, within mutT at 427.248 kb on - strand, within SO0411 at 427.263 kb on + strand, within SO0411 at 427.271 kb on - strand, within SO0411 at 427.278 kb on + strand, within SO0411 at 427.431 kb on + strand at 427.436 kb on + strand at 427.484 kb on + strand at 427.513 kb on + strand at 427.535 kb on + strand at 427.535 kb on + strand at 427.625 kb on + strand, within SO0412 at 427.625 kb on + strand, within SO0412 at 427.647 kb on + strand, within SO0412 at 427.647 kb on + strand, within SO0412 at 427.745 kb on - strand, within SO0412 at 427.747 kb on - strand, within SO0412 at 427.962 kb on - strand, within SO0412 at 427.963 kb on - strand, within SO0412 at 428.053 kb on - strand, within SO0412 at 428.067 kb on + strand, within SO0412 at 428.094 kb on - strand, within SO0412
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.04 mM remove 425,966 + +0.9 426,044 - -0.5 426,118 - -0.4 426,156 + SO0409 0.16 -0.6 426,162 + SO0409 0.17 -0.2 426,164 - SO0409 0.18 +2.3 426,181 + SO0409 0.21 -0.5 426,356 + SO0409 0.53 +0.3 426,369 + SO0409 0.56 +0.7 426,369 + SO0409 0.56 -0.3 426,369 + SO0409 0.56 +0.3 426,369 + SO0409 0.56 +0.4 426,369 + SO0409 0.56 +0.2 426,377 - SO0409 0.57 +0.0 426,377 - SO0409 0.57 +0.6 426,447 - SO0409 0.70 +0.3 426,593 + +0.3 426,719 + -0.2 426,849 + mutT SO0410 0.26 -1.1 426,982 + mutT SO0410 0.60 +0.7 427,050 + mutT SO0410 0.78 +0.6 427,060 - mutT SO0410 0.80 -1.2 427,248 - SO0411 0.11 -0.0 427,263 + SO0411 0.18 -1.1 427,271 - SO0411 0.22 +0.1 427,278 + SO0411 0.25 +0.1 427,431 + +0.2 427,436 + +0.2 427,484 + +0.1 427,513 + -1.5 427,535 + +0.1 427,535 + -0.8 427,625 + SO0412 0.18 -0.6 427,625 + SO0412 0.18 -0.0 427,647 + SO0412 0.21 +0.4 427,647 + SO0412 0.21 +0.2 427,745 - SO0412 0.34 +0.1 427,747 - SO0412 0.34 -0.2 427,962 - SO0412 0.64 +0.4 427,963 - SO0412 0.64 +0.2 428,053 - SO0412 0.76 -0.8 428,067 + SO0412 0.78 +0.4 428,094 - SO0412 0.81 -0.0
Or see this region's nucleotide sequence