Experiment: L-Glutamine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_p187g164 and HP15_p187g165 are separated by 165 nucleotides
HP15_p187g164: HP15_p187g164 - hypothetical protein, at 168,612 to 168,860
_p187g164
HP15_p187g165: HP15_p187g165 - hypothetical protein, at 169,026 to 169,130
_p187g165
Position (kb)
168
169 Strain fitness (log2 ratio)
-1
0
1
2 at 167.665 kb on - strand at 167.710 kb on - strand at 167.711 kb on - strand at 167.859 kb on + strand at 167.915 kb on + strand at 167.942 kb on + strand at 167.995 kb on + strand at 168.014 kb on - strand at 168.027 kb on - strand at 168.062 kb on - strand at 168.094 kb on + strand at 168.155 kb on + strand at 168.155 kb on + strand at 168.247 kb on + strand at 168.290 kb on + strand at 168.436 kb on + strand at 168.436 kb on + strand at 168.436 kb on + strand at 168.444 kb on - strand at 168.497 kb on - strand at 168.525 kb on + strand at 168.568 kb on + strand at 168.609 kb on - strand at 168.609 kb on - strand at 168.636 kb on - strand at 168.663 kb on + strand, within HP15_p187g164 at 168.732 kb on - strand, within HP15_p187g164 at 168.735 kb on + strand, within HP15_p187g164 at 168.743 kb on - strand, within HP15_p187g164 at 168.784 kb on + strand, within HP15_p187g164 at 168.789 kb on - strand, within HP15_p187g164 at 168.809 kb on - strand, within HP15_p187g164 at 168.809 kb on - strand, within HP15_p187g164 at 168.894 kb on + strand at 168.972 kb on + strand at 168.972 kb on + strand at 168.972 kb on - strand at 169.193 kb on + strand at 169.202 kb on + strand at 169.207 kb on + strand at 169.253 kb on - strand at 169.330 kb on + strand at 169.354 kb on - strand at 169.389 kb on + strand at 169.668 kb on - strand at 169.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine as (N) remove 167,665 - -0.8 167,710 - -0.5 167,711 - -0.9 167,859 + +0.4 167,915 + +0.3 167,942 + +0.0 167,995 + -0.5 168,014 - +1.1 168,027 - +0.2 168,062 - -0.1 168,094 + +1.7 168,155 + +0.5 168,155 + -0.4 168,247 + +0.2 168,290 + -0.1 168,436 + +0.5 168,436 + -0.0 168,436 + +2.6 168,444 - -0.0 168,497 - +0.8 168,525 + -0.2 168,568 + -0.3 168,609 - +0.6 168,609 - -0.1 168,636 - +0.1 168,663 + HP15_p187g164 0.20 +2.0 168,732 - HP15_p187g164 0.48 +1.2 168,735 + HP15_p187g164 0.49 -0.4 168,743 - HP15_p187g164 0.53 +1.3 168,784 + HP15_p187g164 0.69 +0.7 168,789 - HP15_p187g164 0.71 -0.1 168,809 - HP15_p187g164 0.79 +1.0 168,809 - HP15_p187g164 0.79 -0.4 168,894 + +0.5 168,972 + +1.1 168,972 + +1.1 168,972 - +0.3 169,193 + +0.0 169,202 + +1.0 169,207 + +1.1 169,253 - +0.1 169,330 + -0.5 169,354 - -1.1 169,389 + +0.9 169,668 - +0.0 169,708 + +2.0
Or see this region's nucleotide sequence