Experiment: L-Glutamine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_362 and HP15_363 are separated by 102 nucleotides HP15_363 and HP15_364 overlap by 4 nucleotides HP15_364 and HP15_365 are separated by 14 nucleotides
HP15_362: HP15_362 - conserved hypothetical protein, secreted, at 362,787 to 365,189
_362
HP15_363: HP15_363 - biotin-protein ligase, at 365,292 to 366,257
_363
HP15_364: HP15_364 - type III pantothenate kinase, at 366,254 to 366,988
_364
HP15_365: HP15_365 - conserved hypothetical protein, at 367,003 to 367,518
_365
Position (kb) 365
366
367 Strain fitness (log2 ratio) -2
-1
0
1 at 364.396 kb on + strand, within HP15_362 at 364.404 kb on - strand, within HP15_362 at 364.408 kb on + strand, within HP15_362 at 364.417 kb on + strand, within HP15_362 at 364.417 kb on + strand, within HP15_362 at 364.425 kb on - strand, within HP15_362 at 364.434 kb on + strand, within HP15_362 at 364.435 kb on - strand, within HP15_362 at 364.440 kb on + strand, within HP15_362 at 364.548 kb on - strand, within HP15_362 at 364.616 kb on + strand, within HP15_362 at 364.651 kb on + strand, within HP15_362 at 364.794 kb on + strand, within HP15_362 at 364.854 kb on - strand, within HP15_362 at 365.035 kb on + strand at 365.339 kb on + strand at 365.339 kb on + strand at 365.477 kb on + strand, within HP15_363 at 365.477 kb on + strand, within HP15_363 at 365.485 kb on - strand, within HP15_363 at 366.208 kb on + strand at 366.246 kb on - strand at 367.003 kb on + strand at 367.129 kb on - strand, within HP15_365 at 367.182 kb on - strand, within HP15_365 at 367.222 kb on - strand, within HP15_365
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine as (N) remove 364,396 + HP15_362 0.67 +0.7 364,404 - HP15_362 0.67 -1.8 364,408 + HP15_362 0.67 +1.0 364,417 + HP15_362 0.68 -0.4 364,417 + HP15_362 0.68 +0.7 364,425 - HP15_362 0.68 -0.3 364,434 + HP15_362 0.69 +1.0 364,435 - HP15_362 0.69 +1.1 364,440 + HP15_362 0.69 +0.7 364,548 - HP15_362 0.73 -0.4 364,616 + HP15_362 0.76 -0.7 364,651 + HP15_362 0.78 +0.6 364,794 + HP15_362 0.84 +0.1 364,854 - HP15_362 0.86 +0.8 365,035 + -0.7 365,339 + -0.9 365,339 + +0.7 365,477 + HP15_363 0.19 -1.3 365,477 + HP15_363 0.19 -0.5 365,485 - HP15_363 0.20 -1.3 366,208 + -0.8 366,246 - -0.0 367,003 + -1.0 367,129 - HP15_365 0.24 -0.2 367,182 - HP15_365 0.35 +0.5 367,222 - HP15_365 0.42 -1.0
Or see this region's nucleotide sequence