Experiment: L-Glutamine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3057 and HP15_3058 are separated by 6 nucleotides HP15_3058 and HP15_3059 are separated by 9 nucleotides HP15_3059 and HP15_3060 are separated by 238 nucleotides
HP15_3057: HP15_3057 - leucine/isoleucine/valine transporter permease subunit, at 3,207,965 to 3,209,236
_3057
HP15_3058: HP15_3058 - leucine/isoleucine/valine transporter ATP-binding subunit, at 3,209,243 to 3,209,998
_3058
HP15_3059: HP15_3059 - high-affinity branched-chain amino acid ABC transporter, ATP-binding protein, at 3,210,008 to 3,210,709
_3059
HP15_3060: HP15_3060 - conserved hypothetical protein, secreted, at 3,210,948 to 3,211,412
_3060
Position (kb)
3209
3210 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3208.279 kb on + strand, within HP15_3057 at 3208.287 kb on - strand, within HP15_3057 at 3208.355 kb on + strand, within HP15_3057 at 3208.358 kb on - strand, within HP15_3057 at 3208.358 kb on - strand, within HP15_3057 at 3208.367 kb on + strand, within HP15_3057 at 3208.393 kb on - strand, within HP15_3057 at 3208.416 kb on - strand, within HP15_3057 at 3208.431 kb on + strand, within HP15_3057 at 3208.439 kb on - strand, within HP15_3057 at 3208.458 kb on - strand, within HP15_3057 at 3208.478 kb on - strand, within HP15_3057 at 3208.488 kb on - strand, within HP15_3057 at 3208.520 kb on - strand, within HP15_3057 at 3208.598 kb on + strand, within HP15_3057 at 3208.656 kb on + strand, within HP15_3057 at 3208.656 kb on + strand, within HP15_3057 at 3208.840 kb on - strand, within HP15_3057 at 3208.843 kb on + strand, within HP15_3057 at 3208.843 kb on + strand, within HP15_3057 at 3208.889 kb on + strand, within HP15_3057 at 3208.961 kb on + strand, within HP15_3057 at 3208.976 kb on - strand, within HP15_3057 at 3208.976 kb on - strand, within HP15_3057 at 3208.976 kb on - strand, within HP15_3057 at 3208.976 kb on - strand, within HP15_3057 at 3209.016 kb on - strand, within HP15_3057 at 3209.148 kb on - strand at 3209.178 kb on - strand at 3209.178 kb on - strand at 3209.484 kb on + strand, within HP15_3058 at 3209.631 kb on - strand, within HP15_3058 at 3209.649 kb on + strand, within HP15_3058 at 3209.649 kb on + strand, within HP15_3058 at 3209.719 kb on - strand, within HP15_3058 at 3209.868 kb on + strand, within HP15_3058 at 3209.868 kb on + strand, within HP15_3058 at 3209.992 kb on + strand at 3210.000 kb on - strand at 3210.007 kb on - strand at 3210.025 kb on + strand at 3210.053 kb on + strand at 3210.136 kb on + strand, within HP15_3059 at 3210.218 kb on + strand, within HP15_3059 at 3210.266 kb on - strand, within HP15_3059 at 3210.380 kb on - strand, within HP15_3059 at 3210.459 kb on + strand, within HP15_3059 at 3210.820 kb on - strand at 3210.824 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine as (N) remove 3,208,279 + HP15_3057 0.25 +2.2 3,208,287 - HP15_3057 0.25 +2.8 3,208,355 + HP15_3057 0.31 +2.4 3,208,358 - HP15_3057 0.31 +2.6 3,208,358 - HP15_3057 0.31 +0.3 3,208,367 + HP15_3057 0.32 +0.6 3,208,393 - HP15_3057 0.34 +0.5 3,208,416 - HP15_3057 0.35 +1.1 3,208,431 + HP15_3057 0.37 +1.1 3,208,439 - HP15_3057 0.37 +0.8 3,208,458 - HP15_3057 0.39 +1.0 3,208,478 - HP15_3057 0.40 +0.7 3,208,488 - HP15_3057 0.41 +1.1 3,208,520 - HP15_3057 0.44 +2.5 3,208,598 + HP15_3057 0.50 +3.6 3,208,656 + HP15_3057 0.54 +2.0 3,208,656 + HP15_3057 0.54 +0.1 3,208,840 - HP15_3057 0.69 +2.5 3,208,843 + HP15_3057 0.69 +1.2 3,208,843 + HP15_3057 0.69 +2.7 3,208,889 + HP15_3057 0.73 +0.8 3,208,961 + HP15_3057 0.78 -0.8 3,208,976 - HP15_3057 0.79 +1.1 3,208,976 - HP15_3057 0.79 +1.1 3,208,976 - HP15_3057 0.79 +0.5 3,208,976 - HP15_3057 0.79 +0.8 3,209,016 - HP15_3057 0.83 +0.6 3,209,148 - +0.8 3,209,178 - +1.6 3,209,178 - +0.4 3,209,484 + HP15_3058 0.32 +2.6 3,209,631 - HP15_3058 0.51 +0.2 3,209,649 + HP15_3058 0.54 +0.7 3,209,649 + HP15_3058 0.54 +0.7 3,209,719 - HP15_3058 0.63 +2.5 3,209,868 + HP15_3058 0.83 +2.5 3,209,868 + HP15_3058 0.83 -0.4 3,209,992 + +0.5 3,210,000 - +0.3 3,210,007 - +0.3 3,210,025 + +0.5 3,210,053 + +2.4 3,210,136 + HP15_3059 0.18 +0.3 3,210,218 + HP15_3059 0.30 +0.2 3,210,266 - HP15_3059 0.37 +2.2 3,210,380 - HP15_3059 0.53 +1.4 3,210,459 + HP15_3059 0.64 +0.6 3,210,820 - -2.3 3,210,824 + +0.3
Or see this region's nucleotide sequence