Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1959 and Shewana3_1960 are separated by 180 nucleotides Shewana3_1960 and Shewana3_1961 are separated by 8 nucleotides Shewana3_1961 and Shewana3_1962 overlap by 4 nucleotides Shewana3_1962 and ansA are separated by 70 nucleotides
Shewana3_1959: Shewana3_1959 - two component transcriptional regulator (RefSeq), at 2,333,086 to 2,333,766
_1959
Shewana3_1960: Shewana3_1960 - peptidase M14, carboxypeptidase A (RefSeq), at 2,333,947 to 2,335,074
_1960
Shewana3_1961: Shewana3_1961 - hypothetical protein (RefSeq), at 2,335,083 to 2,335,400
_1961
Shewana3_1962: Shewana3_1962 - hypothetical protein (RefSeq), at 2,335,397 to 2,335,678
_1962
Shewana3_1963: ansA - cytoplasmic asparaginase I (RefSeq), at 2,335,749 to 2,336,762
ansA
Position (kb)
2333
2334
2335
2336 Strain fitness (log2 ratio)
-1
0
1 at 2333.000 kb on - strand at 2333.194 kb on - strand, within Shewana3_1959 at 2333.213 kb on + strand, within Shewana3_1959 at 2333.291 kb on + strand, within Shewana3_1959 at 2333.432 kb on + strand, within Shewana3_1959 at 2333.432 kb on + strand, within Shewana3_1959 at 2333.705 kb on + strand at 2333.901 kb on + strand at 2334.136 kb on + strand, within Shewana3_1960 at 2334.183 kb on + strand, within Shewana3_1960 at 2334.238 kb on + strand, within Shewana3_1960 at 2334.277 kb on - strand, within Shewana3_1960 at 2334.285 kb on - strand, within Shewana3_1960 at 2334.357 kb on + strand, within Shewana3_1960 at 2334.632 kb on + strand, within Shewana3_1960 at 2334.952 kb on - strand, within Shewana3_1960 at 2335.024 kb on + strand at 2335.558 kb on + strand, within Shewana3_1962 at 2335.845 kb on - strand at 2335.938 kb on - strand, within ansA at 2336.041 kb on - strand, within ansA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 2,333,000 - -0.2 2,333,194 - Shewana3_1959 0.16 +0.5 2,333,213 + Shewana3_1959 0.19 +0.8 2,333,291 + Shewana3_1959 0.30 -1.3 2,333,432 + Shewana3_1959 0.51 -0.7 2,333,432 + Shewana3_1959 0.51 -0.4 2,333,705 + +0.7 2,333,901 + -0.5 2,334,136 + Shewana3_1960 0.17 -0.0 2,334,183 + Shewana3_1960 0.21 +0.8 2,334,238 + Shewana3_1960 0.26 -0.5 2,334,277 - Shewana3_1960 0.29 -1.1 2,334,285 - Shewana3_1960 0.30 +0.1 2,334,357 + Shewana3_1960 0.36 +0.7 2,334,632 + Shewana3_1960 0.61 -0.6 2,334,952 - Shewana3_1960 0.89 -0.1 2,335,024 + -0.7 2,335,558 + Shewana3_1962 0.57 -0.3 2,335,845 - -0.5 2,335,938 - ansA Shewana3_1963 0.19 -1.1 2,336,041 - ansA Shewana3_1963 0.29 -0.0
Or see this region's nucleotide sequence