Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1750 and Shewana3_1751 are separated by 149 nucleotides Shewana3_1751 and clpS are separated by 193 nucleotides clpS and Shewana3_1753 are separated by 42 nucleotides
Shewana3_1750: Shewana3_1750 - isocitrate dehydrogenase, NADP-dependent (RefSeq), at 2,063,884 to 2,066,109
_1750
Shewana3_1751: Shewana3_1751 - cold-shock DNA-binding protein family protein (RefSeq), at 2,066,259 to 2,066,465
_1751
Shewana3_1752: clpS - ATP-dependent Clp protease adaptor protein ClpS (RefSeq), at 2,066,659 to 2,066,967
clpS
Shewana3_1753: Shewana3_1753 - ATP-dependent Clp protease, ATP-binding subunit clpA (RefSeq), at 2,067,010 to 2,069,277
_1753
Position (kb)
2066
2067 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2065.772 kb on - strand, within Shewana3_1750 at 2065.778 kb on - strand, within Shewana3_1750 at 2065.801 kb on + strand, within Shewana3_1750 at 2065.892 kb on - strand at 2065.897 kb on - strand at 2065.972 kb on - strand at 2065.992 kb on + strand at 2066.102 kb on - strand at 2066.179 kb on - strand at 2066.368 kb on + strand, within Shewana3_1751 at 2066.376 kb on - strand, within Shewana3_1751 at 2066.522 kb on - strand at 2066.548 kb on - strand at 2066.946 kb on + strand at 2066.981 kb on + strand at 2067.468 kb on - strand, within Shewana3_1753 at 2067.615 kb on - strand, within Shewana3_1753
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 2,065,772 - Shewana3_1750 0.85 -1.2 2,065,778 - Shewana3_1750 0.85 -1.9 2,065,801 + Shewana3_1750 0.86 -1.9 2,065,892 - -0.6 2,065,897 - -2.9 2,065,972 - -1.9 2,065,992 + +0.1 2,066,102 - -1.5 2,066,179 - +0.7 2,066,368 + Shewana3_1751 0.53 -0.7 2,066,376 - Shewana3_1751 0.57 -1.3 2,066,522 - -2.5 2,066,548 - -1.6 2,066,946 + -0.4 2,066,981 + -2.2 2,067,468 - Shewana3_1753 0.20 +0.2 2,067,615 - Shewana3_1753 0.27 -0.2
Or see this region's nucleotide sequence