Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0256 and Shewana3_0257 are separated by 186 nucleotides Shewana3_0257 and Shewana3_0258 are separated by 213 nucleotides Shewana3_0258 and Shewana3_0259 are separated by 43 nucleotides
Shewana3_0256: Shewana3_0256 - hypothetical protein (RefSeq), at 287,807 to 288,217
_0256
Shewana3_0257: Shewana3_0257 - outer membrane protein, putative (RefSeq), at 288,404 to 288,817
_0257
Shewana3_0258: Shewana3_0258 - nitrogen metabolism transcriptional regulator, NtrC, Fis family (RefSeq), at 289,031 to 290,440
_0258
Shewana3_0259: Shewana3_0259 - signal transduction histidine kinase, nitrogen specific, NtrB (RefSeq), at 290,484 to 291,530
_0259
Position (kb) 289
290
291 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 288.055 kb on - strand, within Shewana3_0256 at 288.093 kb on + strand, within Shewana3_0256 at 288.101 kb on - strand, within Shewana3_0256 at 288.101 kb on - strand, within Shewana3_0256 at 288.102 kb on + strand, within Shewana3_0256 at 288.102 kb on + strand, within Shewana3_0256 at 288.110 kb on - strand, within Shewana3_0256 at 288.138 kb on + strand, within Shewana3_0256 at 288.157 kb on - strand, within Shewana3_0256 at 288.157 kb on - strand, within Shewana3_0256 at 288.384 kb on - strand at 288.389 kb on - strand at 288.460 kb on + strand, within Shewana3_0257 at 288.473 kb on + strand, within Shewana3_0257 at 288.518 kb on + strand, within Shewana3_0257 at 288.519 kb on + strand, within Shewana3_0257 at 288.526 kb on - strand, within Shewana3_0257 at 288.526 kb on - strand, within Shewana3_0257 at 288.528 kb on - strand, within Shewana3_0257 at 288.550 kb on - strand, within Shewana3_0257 at 288.562 kb on - strand, within Shewana3_0257 at 288.706 kb on + strand, within Shewana3_0257 at 288.818 kb on - strand at 288.835 kb on + strand at 289.090 kb on - strand at 289.124 kb on + strand at 289.124 kb on + strand at 289.132 kb on - strand at 289.132 kb on - strand at 289.137 kb on - strand at 289.211 kb on + strand, within Shewana3_0258 at 289.269 kb on - strand, within Shewana3_0258 at 289.286 kb on - strand, within Shewana3_0258 at 289.388 kb on - strand, within Shewana3_0258 at 289.388 kb on - strand, within Shewana3_0258 at 289.471 kb on + strand, within Shewana3_0258 at 290.074 kb on - strand, within Shewana3_0258 at 290.091 kb on - strand, within Shewana3_0258 at 290.131 kb on - strand, within Shewana3_0258 at 290.170 kb on + strand, within Shewana3_0258 at 290.188 kb on - strand, within Shewana3_0258 at 290.188 kb on - strand, within Shewana3_0258 at 290.188 kb on - strand, within Shewana3_0258 at 290.188 kb on - strand, within Shewana3_0258 at 290.199 kb on + strand, within Shewana3_0258 at 290.251 kb on + strand, within Shewana3_0258 at 290.251 kb on + strand, within Shewana3_0258 at 290.251 kb on + strand, within Shewana3_0258 at 290.251 kb on + strand, within Shewana3_0258 at 290.251 kb on + strand, within Shewana3_0258 at 290.259 kb on - strand, within Shewana3_0258 at 290.259 kb on - strand, within Shewana3_0258 at 290.297 kb on - strand, within Shewana3_0258 at 290.357 kb on + strand at 290.528 kb on + strand at 290.536 kb on - strand at 290.597 kb on - strand, within Shewana3_0259 at 290.626 kb on + strand, within Shewana3_0259 at 290.632 kb on + strand, within Shewana3_0259 at 290.640 kb on - strand, within Shewana3_0259 at 290.640 kb on - strand, within Shewana3_0259 at 290.640 kb on - strand, within Shewana3_0259 at 290.675 kb on - strand, within Shewana3_0259 at 290.694 kb on + strand, within Shewana3_0259 at 290.699 kb on + strand, within Shewana3_0259 at 290.707 kb on - strand, within Shewana3_0259 at 290.707 kb on - strand, within Shewana3_0259 at 290.707 kb on - strand, within Shewana3_0259 at 290.778 kb on + strand, within Shewana3_0259 at 290.785 kb on - strand, within Shewana3_0259 at 290.947 kb on - strand, within Shewana3_0259 at 291.083 kb on + strand, within Shewana3_0259 at 291.091 kb on - strand, within Shewana3_0259 at 291.236 kb on - strand, within Shewana3_0259 at 291.236 kb on - strand, within Shewana3_0259 at 291.324 kb on + strand, within Shewana3_0259
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 288,055 - Shewana3_0256 0.60 +0.7 288,093 + Shewana3_0256 0.70 -1.3 288,101 - Shewana3_0256 0.72 -0.3 288,101 - Shewana3_0256 0.72 +0.5 288,102 + Shewana3_0256 0.72 +0.4 288,102 + Shewana3_0256 0.72 -0.4 288,110 - Shewana3_0256 0.74 +0.6 288,138 + Shewana3_0256 0.81 -0.4 288,157 - Shewana3_0256 0.85 -0.8 288,157 - Shewana3_0256 0.85 +0.5 288,384 - -1.0 288,389 - -0.9 288,460 + Shewana3_0257 0.14 -0.2 288,473 + Shewana3_0257 0.17 -0.7 288,518 + Shewana3_0257 0.28 +0.1 288,519 + Shewana3_0257 0.28 -0.7 288,526 - Shewana3_0257 0.29 -0.9 288,526 - Shewana3_0257 0.29 +1.8 288,528 - Shewana3_0257 0.30 +0.3 288,550 - Shewana3_0257 0.35 +0.1 288,562 - Shewana3_0257 0.38 +0.7 288,706 + Shewana3_0257 0.73 -1.1 288,818 - +0.4 288,835 + -0.1 289,090 - -6.1 289,124 + -4.4 289,124 + -3.5 289,132 - -6.5 289,132 - -3.6 289,137 - -4.6 289,211 + Shewana3_0258 0.13 -5.7 289,269 - Shewana3_0258 0.17 -5.0 289,286 - Shewana3_0258 0.18 -3.2 289,388 - Shewana3_0258 0.25 -2.8 289,388 - Shewana3_0258 0.25 -4.5 289,471 + Shewana3_0258 0.31 -5.3 290,074 - Shewana3_0258 0.74 -3.8 290,091 - Shewana3_0258 0.75 -4.3 290,131 - Shewana3_0258 0.78 -3.3 290,170 + Shewana3_0258 0.81 -5.6 290,188 - Shewana3_0258 0.82 -5.7 290,188 - Shewana3_0258 0.82 -5.3 290,188 - Shewana3_0258 0.82 -3.9 290,188 - Shewana3_0258 0.82 -4.5 290,199 + Shewana3_0258 0.83 -3.3 290,251 + Shewana3_0258 0.87 -5.0 290,251 + Shewana3_0258 0.87 -5.1 290,251 + Shewana3_0258 0.87 -3.8 290,251 + Shewana3_0258 0.87 -5.1 290,251 + Shewana3_0258 0.87 -6.9 290,259 - Shewana3_0258 0.87 -1.8 290,259 - Shewana3_0258 0.87 -2.6 290,297 - Shewana3_0258 0.90 -6.1 290,357 + -4.7 290,528 + -5.1 290,536 - -3.1 290,597 - Shewana3_0259 0.11 -2.6 290,626 + Shewana3_0259 0.14 -4.4 290,632 + Shewana3_0259 0.14 -5.1 290,640 - Shewana3_0259 0.15 -5.4 290,640 - Shewana3_0259 0.15 -5.8 290,640 - Shewana3_0259 0.15 -5.5 290,675 - Shewana3_0259 0.18 -4.7 290,694 + Shewana3_0259 0.20 -3.9 290,699 + Shewana3_0259 0.21 -4.0 290,707 - Shewana3_0259 0.21 -4.2 290,707 - Shewana3_0259 0.21 -5.0 290,707 - Shewana3_0259 0.21 -0.8 290,778 + Shewana3_0259 0.28 -4.9 290,785 - Shewana3_0259 0.29 -4.6 290,947 - Shewana3_0259 0.44 -3.6 291,083 + Shewana3_0259 0.57 -5.0 291,091 - Shewana3_0259 0.58 -6.3 291,236 - Shewana3_0259 0.72 -4.5 291,236 - Shewana3_0259 0.72 -3.0 291,324 + Shewana3_0259 0.80 -4.6
Or see this region's nucleotide sequence