Experiment: MoYLS4 with nitrate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0200 and DvMF_0201 are separated by 189 nucleotides DvMF_0201 and DvMF_0202 are separated by 88 nucleotides DvMF_0202 and DvMF_0203 are separated by 237 nucleotides
DvMF_0200: DvMF_0200 - Radical SAM domain protein (RefSeq), at 217,942 to 219,102
_0200
DvMF_0201: DvMF_0201 - hypothetical protein (RefSeq), at 219,292 to 219,768
_0201
DvMF_0202: DvMF_0202 - phosphoribosylaminoimidazole synthetase (RefSeq), at 219,857 to 220,909
_0202
DvMF_0203: DvMF_0203 - Radical SAM domain protein (RefSeq), at 221,147 to 222,682
_0203
Position (kb) 219
220
221 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 218.860 kb on - strand, within DvMF_0200 at 218.995 kb on - strand at 219.184 kb on + strand at 219.325 kb on + strand at 219.333 kb on - strand at 219.886 kb on - strand at 219.958 kb on + strand at 220.270 kb on + strand, within DvMF_0202 at 220.816 kb on - strand at 220.979 kb on + strand at 220.992 kb on - strand at 220.992 kb on - strand at 220.992 kb on - strand at 221.002 kb on - strand at 221.110 kb on + strand at 221.118 kb on - strand at 221.230 kb on - strand at 221.322 kb on - strand, within DvMF_0203 at 221.796 kb on - strand, within DvMF_0203
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with nitrate 50 mM remove 218,860 - DvMF_0200 0.79 +0.2 218,995 - -1.3 219,184 + +0.1 219,325 + -1.2 219,333 - -0.3 219,886 - -3.0 219,958 + -2.0 220,270 + DvMF_0202 0.39 -2.0 220,816 - -1.9 220,979 + +0.4 220,992 - -0.0 220,992 - -0.9 220,992 - +0.3 221,002 - -0.4 221,110 + -0.4 221,118 - -0.5 221,230 - -0.7 221,322 - DvMF_0203 0.11 -0.2 221,796 - DvMF_0203 0.42 -0.2
Or see this region's nucleotide sequence