Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisH and Shew_2202 overlap by 4 nucleotides Shew_2202 and Shew_2203 overlap by 19 nucleotides Shew_2203 and Shew_2204 are separated by 28 nucleotides
Shew_2201: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,562,028 to 2,562,702
hisH
Shew_2202: Shew_2202 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,562,699 to 2,563,436
_2202
Shew_2203: Shew_2203 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,563,418 to 2,564,191
_2203
Shew_2204: Shew_2204 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq), at 2,564,220 to 2,564,840
_2204
Position (kb)
2563
2564
2565 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2562.722 kb on + strand at 2562.800 kb on + strand, within Shew_2202 at 2563.591 kb on - strand, within Shew_2203 at 2563.902 kb on + strand, within Shew_2203 at 2563.973 kb on - strand, within Shew_2203 at 2564.354 kb on - strand, within Shew_2204
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,562,722 + -3.5 2,562,800 + Shew_2202 0.14 -1.4 2,563,591 - Shew_2203 0.22 -4.7 2,563,902 + Shew_2203 0.63 -5.2 2,563,973 - Shew_2203 0.72 -1.8 2,564,354 - Shew_2204 0.22 -2.6
Or see this region's nucleotide sequence