Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2189 and Shew_2190 are separated by 2 nucleotides Shew_2190 and Shew_2191 are separated by 82 nucleotides Shew_2191 and Shew_2192 are separated by 145 nucleotides Shew_2192 and Shew_2193 are separated by 65 nucleotides
Shew_2189: Shew_2189 - alpha/beta hydrolase fold (RefSeq), at 2,548,610 to 2,549,545
_2189
Shew_2190: Shew_2190 - hypothetical protein (RefSeq), at 2,549,548 to 2,550,396
_2190
Shew_2191: Shew_2191 - hypothetical protein (RefSeq), at 2,550,479 to 2,551,180
_2191
Shew_2192: Shew_2192 - hypothetical protein (RefSeq), at 2,551,326 to 2,551,697
_2192
Shew_2193: Shew_2193 - peptidase M22, glycoprotease (RefSeq), at 2,551,763 to 2,552,506
_2193
Position (kb)
2550
2551
2552 Strain fitness (log2 ratio)
-1
0
1 at 2549.516 kb on - strand at 2549.798 kb on - strand, within Shew_2190 at 2549.800 kb on + strand, within Shew_2190 at 2549.800 kb on + strand, within Shew_2190 at 2549.924 kb on - strand, within Shew_2190 at 2549.951 kb on - strand, within Shew_2190 at 2550.651 kb on + strand, within Shew_2191 at 2551.652 kb on + strand, within Shew_2192
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,549,516 - +0.4 2,549,798 - Shew_2190 0.29 -1.5 2,549,800 + Shew_2190 0.30 -0.6 2,549,800 + Shew_2190 0.30 +0.0 2,549,924 - Shew_2190 0.44 +0.2 2,549,951 - Shew_2190 0.47 +0.7 2,550,651 + Shew_2191 0.25 -0.8 2,551,652 + Shew_2192 0.88 -0.3
Or see this region's nucleotide sequence