Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1159 and Shew_1160 are separated by 184 nucleotides Shew_1160 and Shew_1161 are separated by 158 nucleotides Shew_1161 and Shew_1162 are separated by 312 nucleotides
Shew_1159: Shew_1159 - thioesterase superfamily protein (RefSeq), at 1,333,367 to 1,333,735
_1159
Shew_1160: Shew_1160 - hypothetical protein (RefSeq), at 1,333,920 to 1,334,126
_1160
Shew_1161: Shew_1161 - NADH dehydrogenase (RefSeq), at 1,334,285 to 1,335,586
_1161
Shew_1162: Shew_1162 - hypothetical protein (RefSeq), at 1,335,899 to 1,336,273
_1162
Position (kb)
1334
1335
1336 Strain fitness (log2 ratio)
-1
0
1
2 at 1333.582 kb on + strand, within Shew_1159 at 1333.585 kb on - strand, within Shew_1159 at 1333.622 kb on - strand, within Shew_1159 at 1333.727 kb on - strand at 1333.793 kb on - strand at 1333.796 kb on + strand at 1333.806 kb on + strand at 1333.814 kb on - strand at 1334.283 kb on + strand at 1334.653 kb on - strand, within Shew_1161 at 1334.826 kb on + strand, within Shew_1161 at 1335.445 kb on - strand, within Shew_1161 at 1335.952 kb on - strand, within Shew_1162 at 1335.970 kb on - strand, within Shew_1162 at 1336.391 kb on - strand at 1336.419 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,333,582 + Shew_1159 0.58 +0.6 1,333,585 - Shew_1159 0.59 +0.3 1,333,622 - Shew_1159 0.69 -0.0 1,333,727 - +0.2 1,333,793 - -0.6 1,333,796 + +1.9 1,333,806 + -0.3 1,333,814 - +0.1 1,334,283 + +0.4 1,334,653 - Shew_1161 0.28 +0.1 1,334,826 + Shew_1161 0.42 -0.3 1,335,445 - Shew_1161 0.89 +0.3 1,335,952 - Shew_1162 0.14 +0.0 1,335,970 - Shew_1162 0.19 +0.9 1,336,391 - +0.2 1,336,419 + +0.0
Or see this region's nucleotide sequence